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A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2.
Am J Trop Med Hyg. 2020 Dec; 103(6):2350-2352.AJ

Abstract

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia.Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia.Institute for Clinical Research (ICR), National Institutes of Health (NIH), Ministry of Health Malaysia, Putrajaya, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33098286

Citation

Lau, Yee Ling, et al. "A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2." The American Journal of Tropical Medicine and Hygiene, vol. 103, no. 6, 2020, pp. 2350-2352.
Lau YL, Ismail IB, Izati Binti Mustapa N, et al. A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2. Am J Trop Med Hyg. 2020;103(6):2350-2352.
Lau, Y. L., Ismail, I. B., Izati Binti Mustapa, N., Lai, M. Y., Tuan Soh, T. S., Hassan, A. H., Peariasamy, K. M., Lee, Y. L., & Goh, P. P. (2020). A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2. The American Journal of Tropical Medicine and Hygiene, 103(6), 2350-2352. https://doi.org/10.4269/ajtmh.20-1079
Lau YL, et al. A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2. Am J Trop Med Hyg. 2020;103(6):2350-2352. PubMed PMID: 33098286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2. AU - Lau,Yee Ling, AU - Ismail,Ilyiana Binti, AU - Izati Binti Mustapa,Nur, AU - Lai,Meng Yee, AU - Tuan Soh,Tuan Suhaila, AU - Hassan,Afifah Haji, AU - Peariasamy,Kalaiarasu M, AU - Lee,Yee Leng, AU - Goh,Pik Pin, Y1 - 2020/10/23/ PY - 2020/10/25/pubmed PY - 2020/12/17/medline PY - 2020/10/24/entrez SP - 2350 EP - 2352 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 103 IS - 6 N2 - A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/33098286/A_Sensitive_Reverse_Transcription_Loop_Mediated_Isothermal_Amplification_Assay_for_Direct_Visual_Detection_of_SARS_CoV_2_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.20-1079 DB - PRIME DP - Unbound Medicine ER -