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Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2.
Aging (Albany NY). 2020 10 26; 12(20):19880-19897.A

Abstract

Morbidity and mortality of coronavirus disease 2019 (COVID-19) is age-dependent. It remains unclear whether vertical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs during pregnancy and how such infection will affect fetal development. Here, we performed single-cell transcriptomic analysis of placenta and other tissues from fetuses in comparison with those from adults using public-available datasets. Our analysis revealed that a very small proportion of trophoblast cells expressed the Angiotensin I Converting Enzyme 2 (ACE2) gene, suggesting a low possibility of vertical transmission of SARS-CoV-2 from mother to fetus during pregnancy. We found that the fetal adrenal gland, heart, kidney and stomach were susceptible to SARS-CoV-2 infection, because these organs contained cell clusters that expressed high levels of the ACE2 gene. In particular, a higher proportion of ACE2-expressing cell clusters in the adrenal gland and kidney also expressed the Transmembrane Serine Protease 2 (TMPRSS2) gene compared with other organs. Surprisingly, ACE2-expressing type II alveolar (AT2) equivalent cells were absent in fetal lungs. This is in sharp contrast to adult lungs. As ACE2 expression is regulated by various conditions, including oxygen concentration, inflammation and smoking, caution is warranted to avoid triggering potential ACE2 expression in fetal and placental tissue.

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Authors+Show Affiliations

Lü M
Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangzhou, China. Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, Institute of Translational Medicine, School of Basic Medicine, Henan University, Kaifeng, China.
Qiu L
Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Jia G
Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangzhou, China.
Guo R
Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Leng Q
Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangzhou, China.

MeSH

Angiotensin-Converting Enzyme 2BetacoronavirusCOVID-19Coronavirus InfectionsFemaleFetal DiseasesFetusHumansInfectious Disease Transmission, VerticalPandemicsPeptidyl-Dipeptidase APlacentaPneumonia, ViralPregnancyPregnancy Complications, InfectiousSARS-CoV-2Serine EndopeptidasesSingle-Cell Analysis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33104520

Citation

Lü, Mengdie, et al. "Single-cell Expression Profiles of ACE2 and TMPRSS2 Reveals Potential Vertical Transmission and Fetus Infection of SARS-CoV-2." Aging, vol. 12, no. 20, 2020, pp. 19880-19897.
Lü M, Qiu L, Jia G, et al. Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2. Aging (Albany NY). 2020;12(20):19880-19897.
Lü, M., Qiu, L., Jia, G., Guo, R., & Leng, Q. (2020). Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2. Aging, 12(20), 19880-19897. https://doi.org/10.18632/aging.104015
Lü M, et al. Single-cell Expression Profiles of ACE2 and TMPRSS2 Reveals Potential Vertical Transmission and Fetus Infection of SARS-CoV-2. Aging (Albany NY). 2020 10 26;12(20):19880-19897. PubMed PMID: 33104520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2. AU - Lü,Mengdie, AU - Qiu,Li, AU - Jia,Guangshuai, AU - Guo,Rongqun, AU - Leng,Qibin, Y1 - 2020/10/26/ PY - 2020/04/16/received PY - 2020/08/22/accepted PY - 2020/10/27/pubmed PY - 2020/11/24/medline PY - 2020/10/26/entrez KW - COVID-19 KW - SARS-CoV-2 KW - fetus KW - pathogenesis KW - placenta SP - 19880 EP - 19897 JF - Aging JO - Aging (Albany NY) VL - 12 IS - 20 N2 - Morbidity and mortality of coronavirus disease 2019 (COVID-19) is age-dependent. It remains unclear whether vertical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs during pregnancy and how such infection will affect fetal development. Here, we performed single-cell transcriptomic analysis of placenta and other tissues from fetuses in comparison with those from adults using public-available datasets. Our analysis revealed that a very small proportion of trophoblast cells expressed the Angiotensin I Converting Enzyme 2 (ACE2) gene, suggesting a low possibility of vertical transmission of SARS-CoV-2 from mother to fetus during pregnancy. We found that the fetal adrenal gland, heart, kidney and stomach were susceptible to SARS-CoV-2 infection, because these organs contained cell clusters that expressed high levels of the ACE2 gene. In particular, a higher proportion of ACE2-expressing cell clusters in the adrenal gland and kidney also expressed the Transmembrane Serine Protease 2 (TMPRSS2) gene compared with other organs. Surprisingly, ACE2-expressing type II alveolar (AT2) equivalent cells were absent in fetal lungs. This is in sharp contrast to adult lungs. As ACE2 expression is regulated by various conditions, including oxygen concentration, inflammation and smoking, caution is warranted to avoid triggering potential ACE2 expression in fetal and placental tissue. SN - 1945-4589 UR - https://www.unboundmedicine.com/medline/citation/33104520/Single_cell_expression_profiles_of_ACE2_and_TMPRSS2_reveals_potential_vertical_transmission_and_fetus_infection_of_SARS_CoV_2_ DB - PRIME DP - Unbound Medicine ER -