Tags

Type your tag names separated by a space and hit enter

The effects of chrysin and naringin on cyclophosphamide-induced erythrocyte damage in rats: biochemical evaluation of some enzyme activities in vivo and in vitro.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Oct 26 [Online ahead of print]NS

Abstract

In recent years, there have been efforts to develop therapeutic agents that target metabolic enzyme systems in addition to existing treatment in possible cancer treatments. Cyclophosphamide (CYP) is an anticancer drug commonly used in various cancer treatments. Chrysin (CH) and naringin (NR) are natural flavonoids that possess many medicinal and pharmacological properties. In the present study, we aimed to investigate the effect of CH and NR against CYP-induced toxicity on some metabolic enzyme activities. For this purpose, 56 male rats were randomly divided into 8 groups in our in vivo study. The rats were pretreated with CH (25 and 50 mg/kg bw) and NR (50 and 100 mg/kg bw) for 7 days before administering a single dose of CYP (200 mg/kg bw) on the seventh day. According to the in vivo results of our study, it was observed that CH and NR regulated abnormal changes in CYP-induced enzyme activities. In addition, our in vitro study, G6PD enzyme was purified from rat erythrocyte using affinity chromatography. The effects of CH, NR, and CYP were investigated on the purified enzyme. It was determined that CH increased the enzyme activity, CYP ineffective on the enzyme activity, whereas NR inhibited the enzyme activity noncompetitively. Graphical abstract.

Authors+Show Affiliations

Department of Solhan School of Health Services, Solhan Health Services Vocational School, Bingol University, 12000, Bingol, Turkey. ytemel@bingol.edu.tr.Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, 12000, Bingol, Turkey.Department of Chemistry, College of Education, University of Garmian, Kalar, Iraq.Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, 25240, Erzurum, Turkey.Department of Chemistry, College of Education, University of Garmian, Kalar, Iraq.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33104847

Citation

Temel, Yusuf, et al. "The Effects of Chrysin and Naringin On Cyclophosphamide-induced Erythrocyte Damage in Rats: Biochemical Evaluation of some Enzyme Activities in Vivo and in Vitro." Naunyn-Schmiedeberg's Archives of Pharmacology, 2020.
Temel Y, Çağlayan C, Ahmed BM, et al. The effects of chrysin and naringin on cyclophosphamide-induced erythrocyte damage in rats: biochemical evaluation of some enzyme activities in vivo and in vitro. Naunyn Schmiedebergs Arch Pharmacol. 2020.
Temel, Y., Çağlayan, C., Ahmed, B. M., Kandemir, F. M., & Çiftci, M. (2020). The effects of chrysin and naringin on cyclophosphamide-induced erythrocyte damage in rats: biochemical evaluation of some enzyme activities in vivo and in vitro. Naunyn-Schmiedeberg's Archives of Pharmacology. https://doi.org/10.1007/s00210-020-01987-y
Temel Y, et al. The Effects of Chrysin and Naringin On Cyclophosphamide-induced Erythrocyte Damage in Rats: Biochemical Evaluation of some Enzyme Activities in Vivo and in Vitro. Naunyn Schmiedebergs Arch Pharmacol. 2020 Oct 26; PubMed PMID: 33104847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of chrysin and naringin on cyclophosphamide-induced erythrocyte damage in rats: biochemical evaluation of some enzyme activities in vivo and in vitro. AU - Temel,Yusuf, AU - Çağlayan,Cüneyt, AU - Ahmed,Barzan Mirza, AU - Kandemir,Fatih Mehmet, AU - Çiftci,Mehmet, Y1 - 2020/10/26/ PY - 2020/06/24/received PY - 2020/10/05/accepted PY - 2020/10/26/entrez PY - 2020/10/27/pubmed PY - 2020/10/27/medline KW - 6PGD KW - Chrysin KW - Cyclophosphamide KW - G6PD KW - GR KW - Naringin KW - TrxR JF - Naunyn-Schmiedeberg's archives of pharmacology JO - Naunyn Schmiedebergs Arch Pharmacol N2 - In recent years, there have been efforts to develop therapeutic agents that target metabolic enzyme systems in addition to existing treatment in possible cancer treatments. Cyclophosphamide (CYP) is an anticancer drug commonly used in various cancer treatments. Chrysin (CH) and naringin (NR) are natural flavonoids that possess many medicinal and pharmacological properties. In the present study, we aimed to investigate the effect of CH and NR against CYP-induced toxicity on some metabolic enzyme activities. For this purpose, 56 male rats were randomly divided into 8 groups in our in vivo study. The rats were pretreated with CH (25 and 50 mg/kg bw) and NR (50 and 100 mg/kg bw) for 7 days before administering a single dose of CYP (200 mg/kg bw) on the seventh day. According to the in vivo results of our study, it was observed that CH and NR regulated abnormal changes in CYP-induced enzyme activities. In addition, our in vitro study, G6PD enzyme was purified from rat erythrocyte using affinity chromatography. The effects of CH, NR, and CYP were investigated on the purified enzyme. It was determined that CH increased the enzyme activity, CYP ineffective on the enzyme activity, whereas NR inhibited the enzyme activity noncompetitively. Graphical abstract. SN - 1432-1912 UR - https://www.unboundmedicine.com/medline/citation/33104847/The_effects_of_chrysin_and_naringin_on_cyclophosphamide-induced_erythrocyte_damage_in_rats:_biochemical_evaluation_of_some_enzyme_activities_in_vivo_and_in_vitro L2 - https://dx.doi.org/10.1007/s00210-020-01987-y DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.