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The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion.
Vox Sang. 2020 Oct 26 [Online ahead of print]VS

Abstract

BACKGROUND AND OBJECTIVES

Tissue inhibitor of metalloproteinases 2 (TIMP-2) is a protein suspected to be crucial in numerous physiological and pathological processes such as morphogenesis, tissue remodelling and metastasis suppression. In animal models, the administration of TIMP-2 to aged mice improved their cognitive functions. Therefore, one can hypothesize that differences in TIMP-2 levels between blood donors and recipients might influence cognitive functions also in humans. However, the stability of TIMP-2 during processing and storage of blood components for transfusion has not been intensively investigated so far. This study determined TIMP-2 concentrations in fresh-frozen plasma (FFP), erythrocyte concentrate (EC) and pathogen-inactivated platelet concentrate (PI-PC) depending on the donor's demographic factors age and gender.

MATERIALS AND METHODS

Tissue inhibitor of metalloproteinases 2 was measured in FFP (n = 30), EC (n = 12) and PI-PC (n = 12) using a Q-Plex single-plex immunoassay for chemiluminescence-based detection. Absolute quantification of TIMP-2 was performed with Q-view software. Fresh umbilical cord plasma was used as a positive control.

RESULTS

Tissue inhibitor of metalloproteinases 2 was detected in FFP (30/30 samples), EC (11/12 samples) and PI-PC (12/12 samples). The median TIMP-2 concentration in EC (17·2 ng/ml; range: 0-26·5 ng/ml) was significantly lower compared with FFP (63·4 ng/ml; range: 44·4-87·3 ng/ml) or PI-PC (69·9 ng/ml; range: 39·9-83·6 ng/ml). Across all blood components, TIMP-2 levels are comparable in male and female donors and independent of age.

CONCLUSION

Tissue inhibitor of metalloproteinases 2 is detectable and stable in FFP, PI-PC and, in low concentration, EC. It can be hypothesized that TIMP-2 will be transmitted to recipients during transfusion.

Authors+Show Affiliations

Department of Urology, Medical University of Innsbruck, Innsbruck, Austria.Central Institute for Blood Transfusion & Immunological Department, University Hospital of Innsbruck, Innsbruck, Austria.Department of Anesthesiology and Critical Care Medicine, University Hospital of Innsbruck, Innsbruck, Austria.Department of Anesthesiology and Critical Care Medicine, University Hospital of Innsbruck, Innsbruck, Austria.Central Institute for Blood Transfusion & Immunological Department, University Hospital of Innsbruck, Innsbruck, Austria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33107073

Citation

Hoefer, Julia, et al. "The 'rejuvenating Factor' TIMP-2 Is Detectable in Human Blood Components for Transfusion." Vox Sanguinis, 2020.
Hoefer J, Dal-Pont C, Jochberger S, et al. The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion. Vox Sang. 2020.
Hoefer, J., Dal-Pont, C., Jochberger, S., Fantin, R., & Schennach, H. (2020). The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion. Vox Sanguinis. https://doi.org/10.1111/vox.13023
Hoefer J, et al. The 'rejuvenating Factor' TIMP-2 Is Detectable in Human Blood Components for Transfusion. Vox Sang. 2020 Oct 26; PubMed PMID: 33107073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion. AU - Hoefer,Julia, AU - Dal-Pont,Christian, AU - Jochberger,Stefan, AU - Fantin,Raffaella, AU - Schennach,Harald, Y1 - 2020/10/26/ PY - 2020/06/03/received PY - 2020/09/16/revised PY - 2020/10/08/accepted PY - 2020/10/27/entrez PY - 2020/10/28/pubmed PY - 2020/10/28/medline KW - blood components KW - blood transfusion KW - cognitive function KW - morphogenesis KW - tissue inhibitors of metalloproteinases JF - Vox sanguinis JO - Vox Sang N2 - BACKGROUND AND OBJECTIVES: Tissue inhibitor of metalloproteinases 2 (TIMP-2) is a protein suspected to be crucial in numerous physiological and pathological processes such as morphogenesis, tissue remodelling and metastasis suppression. In animal models, the administration of TIMP-2 to aged mice improved their cognitive functions. Therefore, one can hypothesize that differences in TIMP-2 levels between blood donors and recipients might influence cognitive functions also in humans. However, the stability of TIMP-2 during processing and storage of blood components for transfusion has not been intensively investigated so far. This study determined TIMP-2 concentrations in fresh-frozen plasma (FFP), erythrocyte concentrate (EC) and pathogen-inactivated platelet concentrate (PI-PC) depending on the donor's demographic factors age and gender. MATERIALS AND METHODS: Tissue inhibitor of metalloproteinases 2 was measured in FFP (n = 30), EC (n = 12) and PI-PC (n = 12) using a Q-Plex single-plex immunoassay for chemiluminescence-based detection. Absolute quantification of TIMP-2 was performed with Q-view software. Fresh umbilical cord plasma was used as a positive control. RESULTS: Tissue inhibitor of metalloproteinases 2 was detected in FFP (30/30 samples), EC (11/12 samples) and PI-PC (12/12 samples). The median TIMP-2 concentration in EC (17·2 ng/ml; range: 0-26·5 ng/ml) was significantly lower compared with FFP (63·4 ng/ml; range: 44·4-87·3 ng/ml) or PI-PC (69·9 ng/ml; range: 39·9-83·6 ng/ml). Across all blood components, TIMP-2 levels are comparable in male and female donors and independent of age. CONCLUSION: Tissue inhibitor of metalloproteinases 2 is detectable and stable in FFP, PI-PC and, in low concentration, EC. It can be hypothesized that TIMP-2 will be transmitted to recipients during transfusion. SN - 1423-0410 UR - https://www.unboundmedicine.com/medline/citation/33107073/The_'rejuvenating_factor'_TIMP-2_is_detectable_in_human_blood_components_for_transfusion L2 - https://doi.org/10.1111/vox.13023 DB - PRIME DP - Unbound Medicine ER -
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