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Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease.
Sci Rep. 2020 10 27; 10(1):18313.SR

Abstract

Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis.

Authors+Show Affiliations

Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. National Clinical Research Center for Oral Diseases, Shanghai, China. Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. National Clinical Research Center for Oral Diseases, Shanghai, China. Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.National Clinical Research Center for Oral Diseases, Shanghai, China. Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China. Department of Periodontology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.Shanghai Key Laboratory of New Drug Design, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China.Shanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.Shanghai Key Laboratory of New Drug Design, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China. slli403@163.com.Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. sweetzw@hotmail.com. National Clinical Research Center for Oral Diseases, Shanghai, China. sweetzw@hotmail.com. Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China. sweetzw@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33110205

Citation

He, Zhiyan, et al. "Quercetin Inhibits Virulence Properties of Porphyromas Gingivalis in Periodontal Disease." Scientific Reports, vol. 10, no. 1, 2020, p. 18313.
He Z, Zhang X, Song Z, et al. Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease. Sci Rep. 2020;10(1):18313.
He, Z., Zhang, X., Song, Z., Li, L., Chang, H., Li, S., & Zhou, W. (2020). Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease. Scientific Reports, 10(1), 18313. https://doi.org/10.1038/s41598-020-74977-y
He Z, et al. Quercetin Inhibits Virulence Properties of Porphyromas Gingivalis in Periodontal Disease. Sci Rep. 2020 10 27;10(1):18313. PubMed PMID: 33110205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease. AU - He,Zhiyan, AU - Zhang,Xu, AU - Song,Zhongchen, AU - Li,Lu, AU - Chang,Haishuang, AU - Li,Shiliang, AU - Zhou,Wei, Y1 - 2020/10/27/ PY - 2020/07/06/received PY - 2020/10/05/accepted PY - 2020/10/28/entrez PY - 2020/10/29/pubmed PY - 2020/10/29/medline SP - 18313 EP - 18313 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/33110205/Quercetin_inhibits_virulence_properties_of_Porphyromas_gingivalis_in_periodontal_disease L2 - https://doi.org/10.1038/s41598-020-74977-y DB - PRIME DP - Unbound Medicine ER -
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