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SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network.
Mem Inst Oswaldo Cruz. 2020; 115:e200342.MI

Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses.

OBJECTIVES

The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil.

METHODS

After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties.

FINDINGS

The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad.

MAIN CONCLUSION

We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.

Authors+Show Affiliations

Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil. Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Instituto Butantã, Centro de Inovação e Desenvolvimento, Laboratório de Virologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil. Hospital Israelita Albert Einstein, São Paulo, SP, Brasil. Instituto Butantã, Centro de Inovação e Desenvolvimento, Laboratório de Virologia, São Paulo, SP, Brasil.Hospital Israelita Albert Einstein, São Paulo, SP, Brasil. Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Medicina Laboratorial (LIM/03), São Paulo, SP, Brasil. Universidade de São Paulo, Faculdade de Medicina, Departamento de Gastroenterologia, Laboratório de Gastroenterologia Clínica e Experimental (LIM/07), São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brasil. Plataforma Científica Pasteur-USP, São Paulo, SP, Brasil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33111751

Citation

Araujo, Danielle Bastos, et al. "SARS-CoV-2 Isolation From the First Reported Patients in Brazil and Establishment of a Coordinated Task Network." Memorias Do Instituto Oswaldo Cruz, vol. 115, 2020, pp. e200342.
Araujo DB, Machado RRG, Amgarten DE, et al. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Mem Inst Oswaldo Cruz. 2020;115:e200342.
Araujo, D. B., Machado, R. R. G., Amgarten, D. E., Malta, F. M., de Araujo, G. G., Monteiro, C. O., Candido, E. D., Soares, C. P., de Menezes, F. G., Pires, A. C. C., Santana, R. A. F., Viana, A. O., Dorlass, E., Thomazelli, L., Ferreira, L. C. S., Botosso, V. F., Carvalho, C. R. G., Oliveira, D. B. L., Pinho, J. R. R., & Durigon, E. L. (2020). SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Memorias Do Instituto Oswaldo Cruz, 115, e200342. https://doi.org/10.1590/0074-02760200342
Araujo DB, et al. SARS-CoV-2 Isolation From the First Reported Patients in Brazil and Establishment of a Coordinated Task Network. Mem Inst Oswaldo Cruz. 2020;115:e200342. PubMed PMID: 33111751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. AU - Araujo,Danielle Bastos, AU - Machado,Rafael Rahal Guaragna, AU - Amgarten,Deyvid Emanuel, AU - Malta,Fernanda de Mello, AU - de Araujo,Gabriel Guarany, AU - Monteiro,Cairo Oliveira, AU - Candido,Erika Donizetti, AU - Soares,Camila Pereira, AU - de Menezes,Fernando Gatti, AU - Pires,Ana Carolina Cornachioni, AU - Santana,Rúbia Anita Ferraz, AU - Viana,Amanda de Oliveira, AU - Dorlass,Erick, AU - Thomazelli,Luciano, AU - Ferreira,Luis Carlos de Sousa, AU - Botosso,Viviane Fongaro, AU - Carvalho,Cristiane Rodrigues Guzzo, AU - Oliveira,Danielle Bruna Leal, AU - Pinho,João Renato Rebello, AU - Durigon,Edison Luiz, Y1 - 2020/10/23/ PY - 2020/06/30/received PY - 2020/09/24/accepted PY - 2020/10/28/entrez PY - 2020/10/29/pubmed PY - 2020/10/30/medline SP - e200342 EP - e200342 JF - Memorias do Instituto Oswaldo Cruz JO - Mem Inst Oswaldo Cruz VL - 115 N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES: The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS: After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS: The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION: We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research. SN - 1678-8060 UR - https://www.unboundmedicine.com/medline/citation/33111751/SARS-CoV-2_isolation_from_the_first_reported_patients_in_Brazil_and_establishment_of_a_coordinated_task_network L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100344&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -