Tags

Type your tag names separated by a space and hit enter

Anti-Oxidant, Anti-Hemolytic Effects of Crataegus aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line.
Asian Pac J Cancer Prev. 2020 Oct 01; 21(10):2993-3003.AP

Abstract

OBJECTIVE

For Arabian traditional medicine, Crataegus aronia syn. Azarolus (L) Bosc. ex DC (Rosaceae) is widely used to treat diabetes, sexual weakness, cardiovascular diseases and cancer. The anti-cancerous and anti-hemolysis effects of the hydroalcoholic extract of this plant have never been investigated before. The present study aims to evaluate the biological activities of the hydroalcoholic extract of Crataegus aronia leaves in combination with cisplatin, one of the most widely employed chemotherapeutics, on A549 human lung cancer cell line.

METHODS

The anti-oxidant and anti-proliferative activities of leaves, fruits, seeds of C. aronia were investigated by DPPH method and MTT assay; respectively. Cell migration activity was investigated by wound healing and by cell aggregation assays. The effect of C. aronia in inducing cell cycle arrest along with activating cell apoptosis was evaluated by flow cytometry and Western blot assays, respectively.

RESULTS

Our results showed that C. aronia leaves (C. aronia L.) had the highest anti-oxidant and anti-proliferative activities. The leaves extract was potent against hemolysis of the human erythrocytes and showed elevated decrease in migration by reducing wound healing migration and by increasing cell aggregation. Finally, C. aronia L. treatment exhibited apoptotic activity on A549 cells by the down-regulation of PARP-1, caspase-3 and Bcl-2 proteins and by increasing the percentage of A549 cells in sub G0 cell cycle. Moreover, the co-treatment of C. aronia L. and cisplatin remarkably sensitised A549 cells to cisplatin.

CONCLUSION

The results suggested that C. aronia L. could be used as a potential treatment against human lung cancer exhibiting minimal side effects on human health.<br />.

Authors+Show Affiliations

Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Faculty of Sciences, Lebanese University, Lebanon.Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon, Lebanon. Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research, University of Florida, Gainesville, FL, USA.Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Faculty of Sciences, Lebanese University, Lebanon. Department of Biology, Faculty of Sciences-Section I, Lebanese University, Groupe Anti-Cancer Therapeutic Approaches (ATAC), Laboratory Rammal Rammal, Lebanon.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33112559

Citation

Omairi, Islam, et al. "Anti-Oxidant, Anti-Hemolytic Effects of Crataegus Aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 21, no. 10, 2020, pp. 2993-3003.
Omairi I, Kobeissy F, Nasreddine S. Anti-Oxidant, Anti-Hemolytic Effects of Crataegus aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line. Asian Pac J Cancer Prev. 2020;21(10):2993-3003.
Omairi, I., Kobeissy, F., & Nasreddine, S. (2020). Anti-Oxidant, Anti-Hemolytic Effects of Crataegus aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line. Asian Pacific Journal of Cancer Prevention : APJCP, 21(10), 2993-3003. https://doi.org/10.31557/APJCP.2020.21.10.2993
Omairi I, Kobeissy F, Nasreddine S. Anti-Oxidant, Anti-Hemolytic Effects of Crataegus Aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line. Asian Pac J Cancer Prev. 2020 Oct 1;21(10):2993-3003. PubMed PMID: 33112559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-Oxidant, Anti-Hemolytic Effects of Crataegus aronia Leaves and Its Anti- Proliferative Effect Enhance Cisplatin Cytotoxicity in A549 Human Lung Cancer Cell Line. AU - Omairi,Islam, AU - Kobeissy,Firas, AU - Nasreddine,Salam, Y1 - 2020/10/01/ PY - 2020/04/30/received PY - 2020/10/28/entrez PY - 2020/10/29/pubmed PY - 2020/10/29/medline KW - Apoptosis KW - Cell Arrest Cycle KW - Cells aggregation KW - Keywords: Cells Migration SP - 2993 EP - 3003 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 21 IS - 10 N2 - OBJECTIVE: For Arabian traditional medicine, Crataegus aronia syn. Azarolus (L) Bosc. ex DC (Rosaceae) is widely used to treat diabetes, sexual weakness, cardiovascular diseases and cancer. The anti-cancerous and anti-hemolysis effects of the hydroalcoholic extract of this plant have never been investigated before. The present study aims to evaluate the biological activities of the hydroalcoholic extract of Crataegus aronia leaves in combination with cisplatin, one of the most widely employed chemotherapeutics, on A549 human lung cancer cell line. METHODS: The anti-oxidant and anti-proliferative activities of leaves, fruits, seeds of C. aronia were investigated by DPPH method and MTT assay; respectively. Cell migration activity was investigated by wound healing and by cell aggregation assays. The effect of C. aronia in inducing cell cycle arrest along with activating cell apoptosis was evaluated by flow cytometry and Western blot assays, respectively. RESULTS: Our results showed that C. aronia leaves (C. aronia L.) had the highest anti-oxidant and anti-proliferative activities. The leaves extract was potent against hemolysis of the human erythrocytes and showed elevated decrease in migration by reducing wound healing migration and by increasing cell aggregation. Finally, C. aronia L. treatment exhibited apoptotic activity on A549 cells by the down-regulation of PARP-1, caspase-3 and Bcl-2 proteins and by increasing the percentage of A549 cells in sub G0 cell cycle. Moreover, the co-treatment of C. aronia L. and cisplatin remarkably sensitised A549 cells to cisplatin. CONCLUSION: The results suggested that C. aronia L. could be used as a potential treatment against human lung cancer exhibiting minimal side effects on human health.<br />. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/33112559/Anti-Oxidant,_Anti-Hemolytic_Effects_of_Crataegus_aronia_Leaves_and_Its_Anti-_Proliferative_Effect_Enhance_Cisplatin_Cytotoxicity_in_A549_Human_Lung_Cancer_Cell_Line L2 - http://journal.waocp.org/?sid=Entrez:PubMed&amp;id=pmid:33112559&amp;key=2020.21.10.2993 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.