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Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins from Three Aquaria in Japan.
Mycopathologia. 2020 Dec; 185(6):1013-1020.M

Abstract

The skin disease paracoccidioidomycosis ceti occurs in several dolphin species globally. Infection by the unculturable fungi Paracoccidioides brasilensis or other Paracoccidioides spp. results in chronic cutaneous and granulomatous lesions. In this study we used immunohistochemistry to investigate the seroprevalence of antibodies to Paracoccidioides spp. in captive dolphins from three aquaria in Japan. We had previously reported that there were serological cross-reactions for Paracoccidioides spp. with related species in the order Onygenales. We hypothesized that the degree of serological cross-reactions for Paracoccidioides spp. might be lower in areas, such as Japan, where the fungal diseases coccidiodomycosis and paracoccidiodomycosis are not endemic. Sera from 41 apparently healthy dolphins, including 20 Atlantic bottlenose dolphins (BD: Tursiops truncatus), 6 Indo-Pacific bottlenose dolphins (IPBD: Tursiops aduncus), 2 F1 generation of a cross between BD and IPBD (F1), 3 Pacific white-sided dolphins (PWD: Lagenorhynchus obliquidens), 2 pantropical spotted dolphins (PSD: Stenella attenuata), 6 false killer whales (FKW: Pseudorca crassidens), and 2 rough-toothed dolphins (RTD: Steno bredanensis) were investigated. Sera from three dolphins with paracoccidioidomycosis ceti were used as a positive control. The yeast-form cells of Paracoccidioides spp. in the cutaneous tissue sample derived from the first Japanese paracoccidioidomycosis ceti case were used as the antigen for the immunohistochemistry. Of the 41 dolphins tested, 61.0% had antibodies against Paracoccidioides spp. This indicates that dolphins of several species in Japanese aquaria have likely been exposed to the pathogen Paracoccidioides spp.

Authors+Show Affiliations

Okinawa Churashima Foundation, Kunigami-Gun, Aza Ishikawa 888, Motobu-Cho, Okinawa, 905-0206, Japan.Ryukyu Animal Medical Center, Yone 5-24, Tomigusuku, Okinawa, 901-0224, Japan. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.Suma Aqualife Park, Wakamiya-Cho 1-3-5, Suma-Ku, Kobe, Hyogo, 654-0049, Japan.Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinku-Campus, Osaka Prefecture University, Rinku-OraiKita 1-58, Izumisano, Osaka, 598-8531, Japan.Chiran Meat Inspection Center, Kagoshima Prefecture, 22216-1 Minamibeppu Chiran-Cho Minamikyushu-Shi, Kagoshima, 891-0912, Japan.Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Uehara, Nakagusuku-Gun, Nishihara, Okinawa, 903-0215, Japan.Suma Aqualife Park, Wakamiya-Cho 1-3-5, Suma-Ku, Kobe, Hyogo, 654-0049, Japan.Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinku-Campus, Osaka Prefecture University, Rinku-OraiKita 1-58, Izumisano, Osaka, 598-8531, Japan.The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan. Tropical Bioresources Research Center, University of the Ryukyus, Sembaru 1, Nakagusuku-Gun, Nishihara-Cho, Okinawa, 903-0213, Japan. Faculty of Agriculture, University of the Ryukyus, Sembaru 1, Nakagusuku-Gun, Nishihara-Cho, Okinawa, 903-0213, Japan.Ryukyu Animal Medical Center, Yone 5-24, Tomigusuku, Okinawa, 901-0224, Japan. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan. Tropical Bioresources Research Center, University of the Ryukyus, Sembaru 1, Nakagusuku-Gun, Nishihara-Cho, Okinawa, 903-0213, Japan. Faculty of Agriculture, University of the Ryukyus, Sembaru 1, Nakagusuku-Gun, Nishihara-Cho, Okinawa, 903-0213, Japan.Department of Pathological Science, CCB, State University of Londrina, P.O. Box 6001, Londrina, PR, 86051-970, Brazil.Laboratory of Aquatic Medicine, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Kyonan-Cho 1-7-1, Musashino, Tokyo, 180-8602, Japan.College of International Studies, Meio University, 1220-1 Biimata, Nago, Okinawa, 905-8585, Japan.Okinawa Churashima Foundation, Kunigami-Gun, Aza Ishikawa 888, Motobu-Cho, Okinawa, 905-0206, Japan. k-ueda@okichura.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33118124

Citation

Minakawa, Tomoko, et al. "Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins From Three Aquaria in Japan." Mycopathologia, vol. 185, no. 6, 2020, pp. 1013-1020.
Minakawa T, Shumoto G, Kezuka C, et al. Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins from Three Aquaria in Japan. Mycopathologia. 2020;185(6):1013-1020.
Minakawa, T., Shumoto, G., Kezuka, C., Izawa, T., Sasaki, K., Yamaguchi, S., Kamezaki, N., Yamate, J., Konno, T., Sano, A., Itano, E. N., Wada, S., Willson, C., & Ueda, K. (2020). Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins from Three Aquaria in Japan. Mycopathologia, 185(6), 1013-1020. https://doi.org/10.1007/s11046-020-00501-5
Minakawa T, et al. Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins From Three Aquaria in Japan. Mycopathologia. 2020;185(6):1013-1020. PubMed PMID: 33118124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seroprevalence of Antibodies Against Paracoccidioides Spp. in Captive Dolphins from Three Aquaria in Japan. AU - Minakawa,Tomoko, AU - Shumoto,Godai, AU - Kezuka,Chiho, AU - Izawa,Takeshi, AU - Sasaki,Kyoko, AU - Yamaguchi,Sayaka, AU - Kamezaki,Naoki, AU - Yamate,Jyoji, AU - Konno,Toshihiro, AU - Sano,Ayako, AU - Itano,Eiko Nakagawa, AU - Wada,Shinpei, AU - Willson,Chris, AU - Ueda,Keiichi, Y1 - 2020/10/28/ PY - 2019/09/24/received PY - 2020/10/21/accepted PY - 2020/10/30/pubmed PY - 2021/9/4/medline PY - 2020/10/29/entrez KW - Cetacean KW - Dolphin KW - Paracoccidioides spp. KW - Paracoccidioidomycosis ceti KW - Seroprevalence SP - 1013 EP - 1020 JF - Mycopathologia JO - Mycopathologia VL - 185 IS - 6 N2 - The skin disease paracoccidioidomycosis ceti occurs in several dolphin species globally. Infection by the unculturable fungi Paracoccidioides brasilensis or other Paracoccidioides spp. results in chronic cutaneous and granulomatous lesions. In this study we used immunohistochemistry to investigate the seroprevalence of antibodies to Paracoccidioides spp. in captive dolphins from three aquaria in Japan. We had previously reported that there were serological cross-reactions for Paracoccidioides spp. with related species in the order Onygenales. We hypothesized that the degree of serological cross-reactions for Paracoccidioides spp. might be lower in areas, such as Japan, where the fungal diseases coccidiodomycosis and paracoccidiodomycosis are not endemic. Sera from 41 apparently healthy dolphins, including 20 Atlantic bottlenose dolphins (BD: Tursiops truncatus), 6 Indo-Pacific bottlenose dolphins (IPBD: Tursiops aduncus), 2 F1 generation of a cross between BD and IPBD (F1), 3 Pacific white-sided dolphins (PWD: Lagenorhynchus obliquidens), 2 pantropical spotted dolphins (PSD: Stenella attenuata), 6 false killer whales (FKW: Pseudorca crassidens), and 2 rough-toothed dolphins (RTD: Steno bredanensis) were investigated. Sera from three dolphins with paracoccidioidomycosis ceti were used as a positive control. The yeast-form cells of Paracoccidioides spp. in the cutaneous tissue sample derived from the first Japanese paracoccidioidomycosis ceti case were used as the antigen for the immunohistochemistry. Of the 41 dolphins tested, 61.0% had antibodies against Paracoccidioides spp. This indicates that dolphins of several species in Japanese aquaria have likely been exposed to the pathogen Paracoccidioides spp. SN - 1573-0832 UR - https://www.unboundmedicine.com/medline/citation/33118124/Seroprevalence_of_Antibodies_Against_Paracoccidioides_Spp__in_Captive_Dolphins_from_Three_Aquaria_in_Japan_ L2 - https://doi.org/10.1007/s11046-020-00501-5 DB - PRIME DP - Unbound Medicine ER -