Tags

Type your tag names separated by a space and hit enter

High bacterial and viral load in the upper respiratory tract of children in the Democratic Republic of the Congo.
PLoS One. 2020; 15(10):e0240922.Plos

Abstract

BACKGROUND

Respiratory pathogens including Streptococcus pneumoniae and Haemophilus influenzae, are implicated in the pathogenicity of acute lower respiratory infection (ALRI). These are also commonly found in both healthy and sick children. In this study, we describe the first data on the most frequent bacteria and viruses detected in the nasopharynx of children from the general population in the Eastern DR Congo.

METHODS

From January 2014 to June 2015, nasopharyngeal samples from 375 children aged from 2 to 60 months attending health centres for immunisation or growth monitoring were included in the study. Multiplex real-time PCR assays were used for detection of 15 different viruses and 5 bacterial species and for determination of pneumococcal serotypes/serogroups in the nasopharyngeal secretions.

RESULTS

High levels of S. pneumoniae were detected in 77% of cases, and H. influenzae in 51%. Rhinovirus and enterovirus were the most commonly found viruses, while respiratory syncytial virus (RSV) was rare (1%). Co-occurrence of both bacteria and viruses at high levels was detected in 33% of the children. The pneumococcal load was higher in those children who lived in a dwelling with an indoor kitchen area with an open fire, i.e. a kitchen with an open fire for cooking located inside the dwelling with the resultant smoke passing to the living room and/or bedrooms; this was also higher in children from rural areas as compared to children from urban areas or children not living in a dwelling with an indoor kitchen area with an open fire/not living in this type of dwelling. Immunization with 2-3 doses of PCV13 was associated with lower rates of pneumococcal detection. Half of the identified serotypes were non-PCV13 serotypes. The most common non-PCV13 serotypes/serogroups were 15BC, 10A, and 12F, while 5, 6, and 19F were the most prevalent PCV13 serotypes/serogroups.

CONCLUSIONS

The burden of respiratory pathogens including S. pneumoniae in Congolese children was high but relatively few children had RSV. Non-PCV13 serotypes/serogroups became predominant soon after PCV13 was introduced in DR Congo.

Authors+Show Affiliations

Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Panzi Hospital, Bukavu, Democratic Republic of the Congo. Université Evangélique en Afrique, Bukavu, Democratic Republic of the Congo. Institut Superieur Technique Medical, Uvira, Democratic Republic of the Congo.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Region Västra Götaland, Sweden.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.Panzi Hospital, Bukavu, Democratic Republic of the Congo.Panzi Hospital, Bukavu, Democratic Republic of the Congo.Institut Superieur Technique Medical, Uvira, Democratic Republic of the Congo.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Region Västra Götaland, Sweden.Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Region Västra Götaland, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33119683

Citation

Muhandule Birindwa, Archippe, et al. "High Bacterial and Viral Load in the Upper Respiratory Tract of Children in the Democratic Republic of the Congo." PloS One, vol. 15, no. 10, 2020, pp. e0240922.
Muhandule Birindwa A, Gonzales-Siles L, Nordén R, et al. High bacterial and viral load in the upper respiratory tract of children in the Democratic Republic of the Congo. PLoS One. 2020;15(10):e0240922.
Muhandule Birindwa, A., Gonzales-Siles, L., Nordén, R., Geravandi, S., Tumusifu Manegabe, J., Morisho, L., Saili Mushobekwa, S., Andersson, R., & Skovbjerg, S. (2020). High bacterial and viral load in the upper respiratory tract of children in the Democratic Republic of the Congo. PloS One, 15(10), e0240922. https://doi.org/10.1371/journal.pone.0240922
Muhandule Birindwa A, et al. High Bacterial and Viral Load in the Upper Respiratory Tract of Children in the Democratic Republic of the Congo. PLoS One. 2020;15(10):e0240922. PubMed PMID: 33119683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High bacterial and viral load in the upper respiratory tract of children in the Democratic Republic of the Congo. AU - Muhandule Birindwa,Archippe, AU - Gonzales-Siles,Lucia, AU - Nordén,Rickard, AU - Geravandi,Shadi, AU - Tumusifu Manegabe,Jeanière, AU - Morisho,Lambert, AU - Saili Mushobekwa,Stay, AU - Andersson,Rune, AU - Skovbjerg,Susann, Y1 - 2020/10/29/ PY - 2020/04/15/received PY - 2020/10/05/accepted PY - 2020/10/29/entrez PY - 2020/10/30/pubmed PY - 2020/10/30/medline SP - e0240922 EP - e0240922 JF - PloS one JO - PLoS One VL - 15 IS - 10 N2 - BACKGROUND: Respiratory pathogens including Streptococcus pneumoniae and Haemophilus influenzae, are implicated in the pathogenicity of acute lower respiratory infection (ALRI). These are also commonly found in both healthy and sick children. In this study, we describe the first data on the most frequent bacteria and viruses detected in the nasopharynx of children from the general population in the Eastern DR Congo. METHODS: From January 2014 to June 2015, nasopharyngeal samples from 375 children aged from 2 to 60 months attending health centres for immunisation or growth monitoring were included in the study. Multiplex real-time PCR assays were used for detection of 15 different viruses and 5 bacterial species and for determination of pneumococcal serotypes/serogroups in the nasopharyngeal secretions. RESULTS: High levels of S. pneumoniae were detected in 77% of cases, and H. influenzae in 51%. Rhinovirus and enterovirus were the most commonly found viruses, while respiratory syncytial virus (RSV) was rare (1%). Co-occurrence of both bacteria and viruses at high levels was detected in 33% of the children. The pneumococcal load was higher in those children who lived in a dwelling with an indoor kitchen area with an open fire, i.e. a kitchen with an open fire for cooking located inside the dwelling with the resultant smoke passing to the living room and/or bedrooms; this was also higher in children from rural areas as compared to children from urban areas or children not living in a dwelling with an indoor kitchen area with an open fire/not living in this type of dwelling. Immunization with 2-3 doses of PCV13 was associated with lower rates of pneumococcal detection. Half of the identified serotypes were non-PCV13 serotypes. The most common non-PCV13 serotypes/serogroups were 15BC, 10A, and 12F, while 5, 6, and 19F were the most prevalent PCV13 serotypes/serogroups. CONCLUSIONS: The burden of respiratory pathogens including S. pneumoniae in Congolese children was high but relatively few children had RSV. Non-PCV13 serotypes/serogroups became predominant soon after PCV13 was introduced in DR Congo. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/33119683/High_bacterial_and_viral_load_in_the_upper_respiratory_tract_of_children_in_the_Democratic_Republic_of_the_Congo L2 - https://dx.plos.org/10.1371/journal.pone.0240922 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.