Tags

Type your tag names separated by a space and hit enter

High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019.
Viruses. 2020 10 27; 12(11)V

Abstract

MERS-CoV is a zoonotic virus that has emerged in humans in 2012 and caused severe respiratory illness with a mortality rate of 34.4%. Since its appearance, MERS-CoV has been reported in 27 countries and most of these cases were in Saudi Arabia. So far, dromedaries are considered to be the intermediate host and the only known source of human infection. This study was designed to determine the seroprevalence and the infection rate of MERS-CoV in slaughtered food-camels in Riyadh, Saudi Arabia. A total of 171 nasal swabs along with 161 serum samples were collected during the winter; from January to April 2019. Nasal swabs were examined by Rapid test and RT-PCR to detect MERS-CoV RNA, while serum samples were tested primarily using S1-based ELISA Kit to detect MERS-CoV (IgG) antibodies and subsequently by MERS pseudotyped viral particles (MERSpp) neutralization assay for confirmation. Genetic diversity of the positive isolates was determined based on the amplification and sequencing of the spike gene. Our results showed high prevalence (38.6%) of MERS-CoV infection in slaughtered camels and high seropositivity (70.8%) during the time of the study. These data indicate previous and ongoing MERS-CoV infection in camels. Phylogenic analysis revealed relatively low genetic variability among our isolated samples. When these isolates were aligned against published spike sequences of MERS-CoV, deposited in global databases, there was sequence similarity of 94%. High seroprevalence and high genetic stability of MERS-CoV in camels indicating that camels pose a public health threat. The widespread MERS-CoV infections in camels might lead to a risk of future zoonotic transmission into people with direct contact with these infected camels. This study confirms re-infections in camels, highlighting a challenge for vaccine development when it comes to protective immunity.

Authors+Show Affiliations

Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh 11564, Saudi Arabia. College of Science, King Saud University, Riyadh 11564, Saudi Arabia.Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh 11564, Saudi Arabia. King Saud bin Abdulaziz University for Health Sciences, Riyadh 11564, Saudi Arabia.Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh 11564, Saudi Arabia. King Saud bin Abdulaziz University for Health Sciences, Riyadh 11564, Saudi Arabia.Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh 11564, Saudi Arabia. King Saud bin Abdulaziz University for Health Sciences, Riyadh 11564, Saudi Arabia.Department of Medical Laboratories Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia.College of Science, King Saud University, Riyadh 11564, Saudi Arabia.Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh 11564, Saudi Arabia. King Saud bin Abdulaziz University for Health Sciences, Riyadh 11564, Saudi Arabia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33120981

Citation

Aljasim, Taibah A., et al. "High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019." Viruses, vol. 12, no. 11, 2020.
Aljasim TA, Almasoud A, Aljami HA, et al. High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019. Viruses. 2020;12(11).
Aljasim, T. A., Almasoud, A., Aljami, H. A., Alenazi, M. W., Alsagaby, S. A., Alsaleh, A. N., & Alharbi, N. K. (2020). High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019. Viruses, 12(11). https://doi.org/10.3390/v12111215
Aljasim TA, et al. High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019. Viruses. 2020 10 27;12(11) PubMed PMID: 33120981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High Rate of Circulating MERS-CoV in Dromedary Camels at Slaughterhouses in Riyadh, 2019. AU - Aljasim,Taibah A, AU - Almasoud,Abdulrahman, AU - Aljami,Haya A, AU - Alenazi,Mohamed W, AU - Alsagaby,Suliman A, AU - Alsaleh,Asma N, AU - Alharbi,Naif Khalaf, Y1 - 2020/10/27/ PY - 2020/09/12/received PY - 2020/10/02/revised PY - 2020/10/09/accepted PY - 2020/10/30/entrez PY - 2020/10/31/pubmed PY - 2020/11/25/medline KW - ELISA KW - MERS-CoV KW - RT-PCR KW - camel KW - seroprevalence KW - slaughterhouse KW - transmission JF - Viruses JO - Viruses VL - 12 IS - 11 N2 - MERS-CoV is a zoonotic virus that has emerged in humans in 2012 and caused severe respiratory illness with a mortality rate of 34.4%. Since its appearance, MERS-CoV has been reported in 27 countries and most of these cases were in Saudi Arabia. So far, dromedaries are considered to be the intermediate host and the only known source of human infection. This study was designed to determine the seroprevalence and the infection rate of MERS-CoV in slaughtered food-camels in Riyadh, Saudi Arabia. A total of 171 nasal swabs along with 161 serum samples were collected during the winter; from January to April 2019. Nasal swabs were examined by Rapid test and RT-PCR to detect MERS-CoV RNA, while serum samples were tested primarily using S1-based ELISA Kit to detect MERS-CoV (IgG) antibodies and subsequently by MERS pseudotyped viral particles (MERSpp) neutralization assay for confirmation. Genetic diversity of the positive isolates was determined based on the amplification and sequencing of the spike gene. Our results showed high prevalence (38.6%) of MERS-CoV infection in slaughtered camels and high seropositivity (70.8%) during the time of the study. These data indicate previous and ongoing MERS-CoV infection in camels. Phylogenic analysis revealed relatively low genetic variability among our isolated samples. When these isolates were aligned against published spike sequences of MERS-CoV, deposited in global databases, there was sequence similarity of 94%. High seroprevalence and high genetic stability of MERS-CoV in camels indicating that camels pose a public health threat. The widespread MERS-CoV infections in camels might lead to a risk of future zoonotic transmission into people with direct contact with these infected camels. This study confirms re-infections in camels, highlighting a challenge for vaccine development when it comes to protective immunity. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/33120981/High_Rate_of_Circulating_MERS_CoV_in_Dromedary_Camels_at_Slaughterhouses_in_Riyadh_2019_ L2 - https://www.mdpi.com/resolver?pii=v12111215 DB - PRIME DP - Unbound Medicine ER -