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Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells.
Immun Inflamm Dis. 2020 12; 8(4):753-762.II

Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) is a single-stranded RNA virus responsible for the global pandemic of the coronavirus disease-2019 (COVID-19). To date, there are still no effective approaches for the prevention and treatment of COVID-19.

OBJECTIVE

The present study aims to explore the possible mechanisms of SARS-CoV-2 infection in human lung cells.

METHODS

Data interpretation was conducted by recruiting bioinformatics analysis, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways analysis using downloaded data from the NCBI Gene Expression Omnibus database.

RESULTS

The present study demonstrated that SARS-CoV-2 infection induces the upregulation of 14 interferon-stimulated genes, indicative of immune, and interferon responses to the virus. Notably, genes for pyrimidine metabolism and steroid hormone biosynthesis are selectively enriched in human lung cells after SARS-CoV-2 infection, suggesting that altered pyrimidine metabolism and steroid biosynthesis are remarkable, and perhaps druggable features after SARS-CoV-2 infection. Besides, there is a strong positive correlation between viral ORF1ab, ORF6, and angiotensin-converting enzyme 2 (ACE2) expression in human lung cells, implying that ACE2 facilitates SARS-CoV-2 infection and replication in host cells probably through the induction of ORF1ab and ORF6.

Authors+Show Affiliations

Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China. Jinan University, Guangzhou, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.Department of Infectious Diseases and Shenzhen Municipal Key Laboratory for Endogenous Infection, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.Jinan University, Guangzhou, China.Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Hong Kong.Jinan University, Guangzhou, China. Rutgers New Jersey Medical School, Newark, New Jersey, USA.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.Department of Infectious Diseases and Shenzhen Municipal Key Laboratory for Endogenous Infection, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People's Hospital, The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33124193

Citation

Yang, Shaomin, et al. "Transcriptomic Analysis Reveals Novel Mechanisms of SARS-CoV-2 Infection in Human Lung Cells." Immunity, Inflammation and Disease, vol. 8, no. 4, 2020, pp. 753-762.
Yang S, Wu S, Yu Z, et al. Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells. Immun Inflamm Dis. 2020;8(4):753-762.
Yang, S., Wu, S., Yu, Z., Huang, J., Zhong, X., Liu, X., Zhu, H., Xiao, L., Deng, Q., & Sun, W. (2020). Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells. Immunity, Inflammation and Disease, 8(4), 753-762. https://doi.org/10.1002/iid3.366
Yang S, et al. Transcriptomic Analysis Reveals Novel Mechanisms of SARS-CoV-2 Infection in Human Lung Cells. Immun Inflamm Dis. 2020;8(4):753-762. PubMed PMID: 33124193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells. AU - Yang,Shaomin, AU - Wu,Songbin, AU - Yu,Zhijian, AU - Huang,Jiabin, AU - Zhong,Xia, AU - Liu,Xiaodong, AU - Zhu,Hua, AU - Xiao,Lizu, AU - Deng,Qiwen, AU - Sun,Wuping, Y1 - 2020/10/30/ PY - 2020/07/25/received PY - 2020/10/13/revised PY - 2020/10/14/accepted PY - 2020/10/31/pubmed PY - 2020/11/25/medline PY - 2020/10/30/entrez KW - ACE2 KW - COVID-19 KW - SARS-COV-2 KW - coronavirus KW - interferon response KW - pyrimidine metabolism KW - steroid biosynthesis SP - 753 EP - 762 JF - Immunity, inflammation and disease JO - Immun Inflamm Dis VL - 8 IS - 4 N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) is a single-stranded RNA virus responsible for the global pandemic of the coronavirus disease-2019 (COVID-19). To date, there are still no effective approaches for the prevention and treatment of COVID-19. OBJECTIVE: The present study aims to explore the possible mechanisms of SARS-CoV-2 infection in human lung cells. METHODS: Data interpretation was conducted by recruiting bioinformatics analysis, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways analysis using downloaded data from the NCBI Gene Expression Omnibus database. RESULTS: The present study demonstrated that SARS-CoV-2 infection induces the upregulation of 14 interferon-stimulated genes, indicative of immune, and interferon responses to the virus. Notably, genes for pyrimidine metabolism and steroid hormone biosynthesis are selectively enriched in human lung cells after SARS-CoV-2 infection, suggesting that altered pyrimidine metabolism and steroid biosynthesis are remarkable, and perhaps druggable features after SARS-CoV-2 infection. Besides, there is a strong positive correlation between viral ORF1ab, ORF6, and angiotensin-converting enzyme 2 (ACE2) expression in human lung cells, implying that ACE2 facilitates SARS-CoV-2 infection and replication in host cells probably through the induction of ORF1ab and ORF6. SN - 2050-4527 UR - https://www.unboundmedicine.com/medline/citation/33124193/Transcriptomic_analysis_reveals_novel_mechanisms_of_SARS_CoV_2_infection_in_human_lung_cells_ L2 - https://doi.org/10.1002/iid3.366 DB - PRIME DP - Unbound Medicine ER -