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Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis.
BMC Plant Biol. 2020 Oct 31; 20(1):498.BP

Abstract

BACKGROUND

Cuticular wax plays important role in protecting plants from drought stress. In Arabidopsis WRI4 improves drought tolerance by regulating the biosynthesis of fatty acids and cuticular wax. Cyperus esculentus (yellow nutsedge) is a tough weed found in tropical and temperate zones as well as in cooler regions. In the current study, we report the molecular cloning of a WRI4-like gene from Cyperus esculentus and its functional characterization in Arabidopsis.

RESULTS

Using RACE PCR, full-length WRI-like gene was amplified from yellow nutsedge. Phylogenetic analyses and amino acid comparison suggested it to be a WRI4-like gene. According to the tissue-specific expression data, the highest expression of WRI4-like gene was found in leaves, followed by roots and tuber. Transgenic Arabidopsis plants expressing nutsedge WRI4-like gene manifested improved drought stress tolerance. Transgenic lines showed significantly reduced stomatal conductance, transpiration rate, chlorophyll leaching, water loss and improved water use efficiency (WUE). In the absence of drought stress, expression of key genes for fatty acid biosynthesis was not significantly different between transgenic lines and WT while that of cuticular wax biosynthesis genes was significantly higher in transgenic lines than WT. The PEG-simulated drought stress significantly increased expression of key genes for fatty acid as well as wax biosynthesis in transgenic Arabidopsis lines but not in WT plants. Consistent with the gene expression data, cuticular wax load and deposition was significantly higher in stem and leaves of transgenic lines compared with WT under control as well as drought stress conditions.

CONCLUSIONS

WRI4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and deposition. This in turn lowers chlorophyll leaching, stomatal conductance, transpiration rate, water loss and improves water use efficiency under drought stress conditions. Therefore, CeWRI4-like gene could be a good candidate for improving drought tolerance in crops.

Authors+Show Affiliations

State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China.Biotechnology Department, COMSATS University Islamabad, Abbottabad Campus 22060, University Road, Abbottabad, Pakistan.Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, 434023, China. zhang.xk@139.com.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life Science, Hubei University, Wuhan, 430062, China. huangbangquan@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33129252

Citation

Cheng, Chao, et al. "Yellow Nutsedge WRI4-like Gene Improves Drought Tolerance in Arabidopsis Thaliana By Promoting Cuticular Wax Biosynthesis." BMC Plant Biology, vol. 20, no. 1, 2020, p. 498.
Cheng C, Hu S, Han Y, et al. Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis. BMC Plant Biol. 2020;20(1):498.
Cheng, C., Hu, S., Han, Y., Xia, D., Huang, B. L., Wu, W., Hussain, J., Zhang, X., & Huang, B. (2020). Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis. BMC Plant Biology, 20(1), 498. https://doi.org/10.1186/s12870-020-02707-7
Cheng C, et al. Yellow Nutsedge WRI4-like Gene Improves Drought Tolerance in Arabidopsis Thaliana By Promoting Cuticular Wax Biosynthesis. BMC Plant Biol. 2020 Oct 31;20(1):498. PubMed PMID: 33129252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Yellow nutsedge WRI4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis. AU - Cheng,Chao, AU - Hu,Shutong, AU - Han,Yun, AU - Xia,Di, AU - Huang,Bang-Lian, AU - Wu,Wenhua, AU - Hussain,Jamshaid, AU - Zhang,Xuekun, AU - Huang,Bangquan, Y1 - 2020/10/31/ PY - 2019/11/22/received PY - 2020/10/19/accepted PY - 2020/11/1/entrez PY - 2020/11/2/pubmed PY - 2021/3/24/medline KW - Cuticular wax biosynthesis KW - Cyperus esculentus KW - Drought tolerance KW - Gene expression KW - WRI4-like gene SP - 498 EP - 498 JF - BMC plant biology JO - BMC Plant Biol VL - 20 IS - 1 N2 - BACKGROUND: Cuticular wax plays important role in protecting plants from drought stress. In Arabidopsis WRI4 improves drought tolerance by regulating the biosynthesis of fatty acids and cuticular wax. Cyperus esculentus (yellow nutsedge) is a tough weed found in tropical and temperate zones as well as in cooler regions. In the current study, we report the molecular cloning of a WRI4-like gene from Cyperus esculentus and its functional characterization in Arabidopsis. RESULTS: Using RACE PCR, full-length WRI-like gene was amplified from yellow nutsedge. Phylogenetic analyses and amino acid comparison suggested it to be a WRI4-like gene. According to the tissue-specific expression data, the highest expression of WRI4-like gene was found in leaves, followed by roots and tuber. Transgenic Arabidopsis plants expressing nutsedge WRI4-like gene manifested improved drought stress tolerance. Transgenic lines showed significantly reduced stomatal conductance, transpiration rate, chlorophyll leaching, water loss and improved water use efficiency (WUE). In the absence of drought stress, expression of key genes for fatty acid biosynthesis was not significantly different between transgenic lines and WT while that of cuticular wax biosynthesis genes was significantly higher in transgenic lines than WT. The PEG-simulated drought stress significantly increased expression of key genes for fatty acid as well as wax biosynthesis in transgenic Arabidopsis lines but not in WT plants. Consistent with the gene expression data, cuticular wax load and deposition was significantly higher in stem and leaves of transgenic lines compared with WT under control as well as drought stress conditions. CONCLUSIONS: WRI4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and deposition. This in turn lowers chlorophyll leaching, stomatal conductance, transpiration rate, water loss and improves water use efficiency under drought stress conditions. Therefore, CeWRI4-like gene could be a good candidate for improving drought tolerance in crops. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/33129252/Yellow_nutsedge_WRI4_like_gene_improves_drought_tolerance_in_Arabidopsis_thaliana_by_promoting_cuticular_wax_biosynthesis_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02707-7 DB - PRIME DP - Unbound Medicine ER -