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Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular injury following by testicular torsion/detorsion in male rats.
Exp Mol Pathol. 2020 12; 117:104563.EM

Abstract

PURPOSE

This study was designed to determine the probable protection mechanisms of nitroglycerin, a widely used medication for treatment of heart failure and angina, in amelioration of testicular ischemia/reperfusion damage.

METHODS

24 adult male rats were randomly divided into three equal groups; with eight rats in each group: Group 1 (Sham) was sham-operated. Group 2 (T_D): 2 h testicular torsion was induced, afterward detorsion was performed and maintained for 2 h. Group 3 (NG): Nitroglycerin was administered immediately after detorsion. Sperm quality parameters such as viability, motility, morphology, and concentration, levels of antioxidant enzymes (glutathione peroxidase (GPX), catalase (CAT), and total antioxidant capacity (TAC)), and amount of malondialdehyde (MDA) in the blood plasma were examined in each group, thereafter histopathological parameters including germinal epithelial cell thickness (GECT), mean seminiferous tubular diameter (MSTD), Johnson's score and Cosentino's score were assessed.

RESULTS

Testicular T_D significantly reduced sperm viability, motility, and normal morphology, whereas the NG administration remarkably increased the percentage of live, motile, and normal spermatozoa (p < 0.05). Levels of GPx, CAT, and TAC significantly reduced and the MDA level significantly increased in the T_D group in comparison to the sham group (p < 0.05). The NG treated group demonstrated significantly reduced MDA concentrations as well as elevated levels of GPx and CAT compared to the T_D group (p < 0.05). Induction of testicular torsion significantly reduced Johnson's score, GESCT (μm), and MSTD (μm), and remarkably increased the Cosentino's score (P < 0.05), while NG injection significantly increased Johnson's score, GESCT (μm), and MSTD (μm) and reduced the Cosentino's score (P < 0.05).

CONCLUSION

According to the findings in this research, nitroglycerin was able to protect the testicular tissue from ischemia-reperfusion damage caused by induced torsion/detorsion.

Authors+Show Affiliations

Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran. Electronic address: raisi.a@lu.ac.ir.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33147433

Citation

Raisi, Abbas, et al. "Nitroglycerin Ameliorates Sperm Parameters, Oxidative Stress and Testicular Injury Following By Testicular Torsion/detorsion in Male Rats." Experimental and Molecular Pathology, vol. 117, 2020, p. 104563.
Raisi A, Kheradmand A, Farjanikish G, et al. Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular injury following by testicular torsion/detorsion in male rats. Exp Mol Pathol. 2020;117:104563.
Raisi, A., Kheradmand, A., Farjanikish, G., Davoodi, F., & Taheri, S. (2020). Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular injury following by testicular torsion/detorsion in male rats. Experimental and Molecular Pathology, 117, 104563. https://doi.org/10.1016/j.yexmp.2020.104563
Raisi A, et al. Nitroglycerin Ameliorates Sperm Parameters, Oxidative Stress and Testicular Injury Following By Testicular Torsion/detorsion in Male Rats. Exp Mol Pathol. 2020;117:104563. PubMed PMID: 33147433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular injury following by testicular torsion/detorsion in male rats. AU - Raisi,Abbas, AU - Kheradmand,Arash, AU - Farjanikish,Ghasem, AU - Davoodi,Farshid, AU - Taheri,Shayan, Y1 - 2020/11/02/ PY - 2020/04/21/received PY - 2020/08/18/revised PY - 2020/10/28/accepted PY - 2020/11/5/pubmed PY - 2021/2/3/medline PY - 2020/11/4/entrez KW - Ischemia-reperfusion KW - Nitroglycerin KW - Oxidative stress KW - Sperm parameters KW - Testicular torsion detorsion SP - 104563 EP - 104563 JF - Experimental and molecular pathology JO - Exp Mol Pathol VL - 117 N2 - PURPOSE: This study was designed to determine the probable protection mechanisms of nitroglycerin, a widely used medication for treatment of heart failure and angina, in amelioration of testicular ischemia/reperfusion damage. METHODS: 24 adult male rats were randomly divided into three equal groups; with eight rats in each group: Group 1 (Sham) was sham-operated. Group 2 (T_D): 2 h testicular torsion was induced, afterward detorsion was performed and maintained for 2 h. Group 3 (NG): Nitroglycerin was administered immediately after detorsion. Sperm quality parameters such as viability, motility, morphology, and concentration, levels of antioxidant enzymes (glutathione peroxidase (GPX), catalase (CAT), and total antioxidant capacity (TAC)), and amount of malondialdehyde (MDA) in the blood plasma were examined in each group, thereafter histopathological parameters including germinal epithelial cell thickness (GECT), mean seminiferous tubular diameter (MSTD), Johnson's score and Cosentino's score were assessed. RESULTS: Testicular T_D significantly reduced sperm viability, motility, and normal morphology, whereas the NG administration remarkably increased the percentage of live, motile, and normal spermatozoa (p < 0.05). Levels of GPx, CAT, and TAC significantly reduced and the MDA level significantly increased in the T_D group in comparison to the sham group (p < 0.05). The NG treated group demonstrated significantly reduced MDA concentrations as well as elevated levels of GPx and CAT compared to the T_D group (p < 0.05). Induction of testicular torsion significantly reduced Johnson's score, GESCT (μm), and MSTD (μm), and remarkably increased the Cosentino's score (P < 0.05), while NG injection significantly increased Johnson's score, GESCT (μm), and MSTD (μm) and reduced the Cosentino's score (P < 0.05). CONCLUSION: According to the findings in this research, nitroglycerin was able to protect the testicular tissue from ischemia-reperfusion damage caused by induced torsion/detorsion. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/33147433/Nitroglycerin_ameliorates_sperm_parameters_oxidative_stress_and_testicular_injury_following_by_testicular_torsion/detorsion_in_male_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(20)30840-6 DB - PRIME DP - Unbound Medicine ER -