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Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats.
J Ethnopharmacol. 2021 Mar 25; 268:113577.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Launaea acanthodes (Boiss.) O. Kuntze is native to semiarid regions of central Iran, traditionally used in the treatment of numerous disorders including diabetes.

AIM OF THE STUDY

The current study aimed to explore hypoglycemic activity of Launaea acanthodes extract in streptozotocin-induced diabetic rats. Furthermore, gene expression study was carried out to examine expression levels of key glucose metabolism-related genes.

METHODS

For in vitro study, Folin-Ciocalteus, DPPH and aluminum chloride colorimetric assays were used to determine the total phenolic content, antioxidant capacity and total flavonoid content of extracts, respectively. For in vivo study, streptozotocin-induced diabetic Wistar rats were orally administered with metformin (50 mg/kg) and various doses of extracts (100, 200 and 400 mg/kg body weight) for 28 days. Fasting blood glucose, body weight, food and water intake were assessed during the course of treatment. At the end of the intervention, oral glucose tolerance test (OGTT), lipid profile and glycated hemoglobin (HbA1c) were evaluated. Furthermore, functional liver enzymes, oxidative stress markers and histopathology of pancreas were examined. Lastly, quantitative real time polymerase chain reaction (qRT-PCR) was applied to explore the mRNA levels of genes relevant to glucose metabolism in the pancreas and liver tissues of diabetic rats.

RESULTS

Based on the in vitro results, the hydroalcoholic extract revealed potential radical scavenging activity and contained highest amount of phenolic and flavonoid. The in vivo results demonstrated that the extract lowered fasting blood glucose level, increased the body weight, restored the alterations in the levels of water and food intake, attenuated HbA1c, improved lipid profile and ameliorated the OGTT in diabetic rats. The extract administration alleviated the histopathological changes in the pancreas, suppressed malondialdehyde (MDA) level and further restored attenuated levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in diabetic rats. Analysis of real time PCR data showed that extract administration reversed the expression levels of hepatic glucokinase (GK), phosphenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). Meanwhile, the extract upregulated the expression level of glucose transporter-2 (GLUT-2) and pancreatic-duodenal homeobox (PDX-1) in diabetic rats.

CONCLUSION

Collectively, the results demonstrate that Launaea acanthodes hydroalcoholic extract exerts hypoglycemic effect possibly via regulating key enzymes of glucose metabolism and ameliorating pancreatic dysfunction through its antioxidant properties.

Authors+Show Affiliations

Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: sm_hosseini@bmsu.ac.ir.Department of Biology, Kavian Institute of Higher Education, Mashhad, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33171271

Citation

Marvibaigi, Mohsen, et al. "Launaea Acanthodes (Boiss) O. Kuntze Mediates Hepatic Glucose Metabolism and Ameliorates Impaired Pancreatic Function in Streptozotocin-induced Diabetic Rats." Journal of Ethnopharmacology, vol. 268, 2021, p. 113577.
Marvibaigi M, Hosseini SM, Amini N. Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2021;268:113577.
Marvibaigi, M., Hosseini, S. M., & Amini, N. (2021). Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 268, 113577. https://doi.org/10.1016/j.jep.2020.113577
Marvibaigi M, Hosseini SM, Amini N. Launaea Acanthodes (Boiss) O. Kuntze Mediates Hepatic Glucose Metabolism and Ameliorates Impaired Pancreatic Function in Streptozotocin-induced Diabetic Rats. J Ethnopharmacol. 2021 Mar 25;268:113577. PubMed PMID: 33171271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats. AU - Marvibaigi,Mohsen, AU - Hosseini,Seyed Morteza, AU - Amini,Neda, Y1 - 2020/11/07/ PY - 2020/08/16/received PY - 2020/10/26/revised PY - 2020/11/06/accepted PY - 2020/11/11/pubmed PY - 2021/3/6/medline PY - 2020/11/10/entrez KW - Diabetes mellitus KW - Glucose metabolism KW - Hypoglycemic activity KW - Launaea acanthodes KW - Oxidative stress SP - 113577 EP - 113577 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 268 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Launaea acanthodes (Boiss.) O. Kuntze is native to semiarid regions of central Iran, traditionally used in the treatment of numerous disorders including diabetes. AIM OF THE STUDY: The current study aimed to explore hypoglycemic activity of Launaea acanthodes extract in streptozotocin-induced diabetic rats. Furthermore, gene expression study was carried out to examine expression levels of key glucose metabolism-related genes. METHODS: For in vitro study, Folin-Ciocalteus, DPPH and aluminum chloride colorimetric assays were used to determine the total phenolic content, antioxidant capacity and total flavonoid content of extracts, respectively. For in vivo study, streptozotocin-induced diabetic Wistar rats were orally administered with metformin (50 mg/kg) and various doses of extracts (100, 200 and 400 mg/kg body weight) for 28 days. Fasting blood glucose, body weight, food and water intake were assessed during the course of treatment. At the end of the intervention, oral glucose tolerance test (OGTT), lipid profile and glycated hemoglobin (HbA1c) were evaluated. Furthermore, functional liver enzymes, oxidative stress markers and histopathology of pancreas were examined. Lastly, quantitative real time polymerase chain reaction (qRT-PCR) was applied to explore the mRNA levels of genes relevant to glucose metabolism in the pancreas and liver tissues of diabetic rats. RESULTS: Based on the in vitro results, the hydroalcoholic extract revealed potential radical scavenging activity and contained highest amount of phenolic and flavonoid. The in vivo results demonstrated that the extract lowered fasting blood glucose level, increased the body weight, restored the alterations in the levels of water and food intake, attenuated HbA1c, improved lipid profile and ameliorated the OGTT in diabetic rats. The extract administration alleviated the histopathological changes in the pancreas, suppressed malondialdehyde (MDA) level and further restored attenuated levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in diabetic rats. Analysis of real time PCR data showed that extract administration reversed the expression levels of hepatic glucokinase (GK), phosphenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). Meanwhile, the extract upregulated the expression level of glucose transporter-2 (GLUT-2) and pancreatic-duodenal homeobox (PDX-1) in diabetic rats. CONCLUSION: Collectively, the results demonstrate that Launaea acanthodes hydroalcoholic extract exerts hypoglycemic effect possibly via regulating key enzymes of glucose metabolism and ameliorating pancreatic dysfunction through its antioxidant properties. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/33171271/Launaea_acanthodes__Boiss__O__Kuntze_mediates_hepatic_glucose_metabolism_and_ameliorates_impaired_pancreatic_function_in_streptozotocin_induced_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(20)33465-6 DB - PRIME DP - Unbound Medicine ER -