Tags

Type your tag names separated by a space and hit enter

Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes.
Viruses. 2020 11 07; 12(11)V

Abstract

Marseilleviridae members are large dsDNA viruses with icosahedral particles 250 nm in diameter infecting Acanthamoeba. Their 340 to 390 kb genomes encode 450 to 550 protein-coding genes. Since the discovery of marseillevirus (the prototype of the family) in 2009, several strains were isolated from various locations, among which 13 are now fully sequenced. This allows the organization of their genomes to be deciphered through comparative genomics. Here, we first experimentally demonstrate that the Marseilleviridae genomes are circular. We then acknowledge a strong bias in sequence conservation, revealing two distinct genomic regions. One gathers most Marseilleviridae paralogs and has undergone genomic rearrangements, while the other, enriched in core genes, exhibits the opposite pattern. Most of the genes whose protein products compose the viral particles are located in the conserved region. They are also strongly biased toward a late gene expression pattern. We finally discuss the potential advantages of Marseilleviridae having a circular genome, and the possible link between the biased distribution of their genes and the transcription as well as DNA replication mechanisms that remain to be characterized.

Authors+Show Affiliations

CNRS, IGS, Information Génomique & Structurale (UMR7256), Institut de Microbiologie de la Méditerranée (FR 3489), Aix Marseille Univ., 13288 Marseille, France.CNRS, IGS, Information Génomique & Structurale (UMR7256), Institut de Microbiologie de la Méditerranée (FR 3489), Aix Marseille Univ., 13288 Marseille, France.CNRS, IGS, Information Génomique & Structurale (UMR7256), Institut de Microbiologie de la Méditerranée (FR 3489), Aix Marseille Univ., 13288 Marseille, France.CNRS, IGS, Information Génomique & Structurale (UMR7256), Institut de Microbiologie de la Méditerranée (FR 3489), Aix Marseille Univ., 13288 Marseille, France.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33171839

Citation

Blanca, Léo, et al. "Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes." Viruses, vol. 12, no. 11, 2020.
Blanca L, Christo-Foroux E, Rigou S, et al. Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes. Viruses. 2020;12(11).
Blanca, L., Christo-Foroux, E., Rigou, S., & Legendre, M. (2020). Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes. Viruses, 12(11). https://doi.org/10.3390/v12111270
Blanca L, et al. Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes. Viruses. 2020 11 7;12(11) PubMed PMID: 33171839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Analysis of the Circular and Highly Asymmetrical Marseilleviridae Genomes. AU - Blanca,Léo, AU - Christo-Foroux,Eugène, AU - Rigou,Sofia, AU - Legendre,Matthieu, Y1 - 2020/11/07/ PY - 2020/10/07/received PY - 2020/11/05/revised PY - 2020/11/05/accepted PY - 2020/11/11/entrez PY - 2020/11/12/pubmed PY - 2021/3/4/medline KW - comparative genomics KW - genome evolution KW - large DNA viruses KW - marseillevirus JF - Viruses JO - Viruses VL - 12 IS - 11 N2 - Marseilleviridae members are large dsDNA viruses with icosahedral particles 250 nm in diameter infecting Acanthamoeba. Their 340 to 390 kb genomes encode 450 to 550 protein-coding genes. Since the discovery of marseillevirus (the prototype of the family) in 2009, several strains were isolated from various locations, among which 13 are now fully sequenced. This allows the organization of their genomes to be deciphered through comparative genomics. Here, we first experimentally demonstrate that the Marseilleviridae genomes are circular. We then acknowledge a strong bias in sequence conservation, revealing two distinct genomic regions. One gathers most Marseilleviridae paralogs and has undergone genomic rearrangements, while the other, enriched in core genes, exhibits the opposite pattern. Most of the genes whose protein products compose the viral particles are located in the conserved region. They are also strongly biased toward a late gene expression pattern. We finally discuss the potential advantages of Marseilleviridae having a circular genome, and the possible link between the biased distribution of their genes and the transcription as well as DNA replication mechanisms that remain to be characterized. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/33171839/Comparative_Analysis_of_the_Circular_and_Highly_Asymmetrical_Marseilleviridae_Genomes_ L2 - https://www.mdpi.com/resolver?pii=v12111270 DB - PRIME DP - Unbound Medicine ER -