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Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19.
Nat Commun. 2020 11 11; 11(1):5703.NC

Abstract

Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. Here, we describe clinical features, virology, longitudinal cellular, and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody responses in a family of two parents with PCR-confirmed symptomatic SARS-CoV-2 infection and their three children, who tested repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children are similar to their parents at all timepoints. All family members have salivary anti-SARS-CoV-2 antibodies detected, predominantly IgA, that coincide with symptom resolution in 3 of 4 symptomatic members. Plasma from both parents and one child have IgG antibody against the S1 protein and virus-neutralizing activity detected. Using a systems serology approach, we demonstrate higher levels of SARS-CoV-2-specific antibody features of these family members compared to healthy controls. These data indicate that children can mount an immune response to SARS-CoV-2 without virological confirmation of infection, raising the possibility that immunity in children can prevent the establishment of SARS-CoV-2 infection. Relying on routine virological and serological testing may not identify exposed children, with implications for epidemiological and clinical studies across the life-span.

Authors+Show Affiliations

Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Shidan.Tosif@rch.org.au. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Shidan.Tosif@rch.org.au. Department of General Medicine, The Royal Children's Hospital, Melbourne, Victoria, Australia. Shidan.Tosif@rch.org.au.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia. Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.Department of Biomedical Engineering, University of Michigan, MI, USA.Department of Biomedical Engineering, University of Michigan, MI, USA.Department of Biomedical Engineering, University of Michigan, MI, USA.Department of Infectious Diseases, Launceston General Hospital, Launceston, Tasmania, Australia. School of Health Sciences and School of Medicine, University of Tasmania, Launceston, Tasmania, Australia. Department of Immunology and Pathology, Monash University, Commercial Road, Melbourne, Victoria, Australia. School of Health and Biomedical Science, RMIT University, Melbourne, Victoria, Australia.Department of Biomedical Engineering, University of Michigan, MI, USA.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Infectious Diseases Unit, Department of General Medicine, The Royal Children's Hospital, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Department of Gastroenterology, The Royal Children's Hospital, Melbourne, Victoria, Australia.Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Infectious Diseases Unit, Department of General Medicine, The Royal Children's Hospital, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Department of General Medicine, The Royal Children's Hospital, Melbourne, Victoria, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Infectious Diseases Unit, Department of General Medicine, The Royal Children's Hospital, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia. WHO Collaborating Centre for Reference and Research on Influenza, Melbourne, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Infectious Diseases Unit, Department of General Medicine, The Royal Children's Hospital, Melbourne, Australia.Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia. Infection and Immunity, Murdoch Children's Research Institute, Melbourne, Australia. Department of General Medicine, The Royal Children's Hospital, Melbourne, Victoria, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33177504

Citation

Tosif, Shidan, et al. "Immune Responses to SARS-CoV-2 in Three Children of Parents With Symptomatic COVID-19." Nature Communications, vol. 11, no. 1, 2020, p. 5703.
Tosif S, Neeland MR, Sutton P, et al. Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. Nat Commun. 2020;11(1):5703.
Tosif, S., Neeland, M. R., Sutton, P., Licciardi, P. V., Sarkar, S., Selva, K. J., Do, L. A. H., Donato, C., Quan Toh, Z., Higgins, R., Van de Sandt, C., Lemke, M. M., Lee, C. Y., Shoffner, S. K., Flanagan, K. L., Arnold, K. B., Mordant, F. L., Mulholland, K., Bines, J., ... Crawford, N. W. (2020). Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. Nature Communications, 11(1), 5703. https://doi.org/10.1038/s41467-020-19545-8
Tosif S, et al. Immune Responses to SARS-CoV-2 in Three Children of Parents With Symptomatic COVID-19. Nat Commun. 2020 11 11;11(1):5703. PubMed PMID: 33177504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. AU - Tosif,Shidan, AU - Neeland,Melanie R, AU - Sutton,Philip, AU - Licciardi,Paul V, AU - Sarkar,Sohinee, AU - Selva,Kevin J, AU - Do,Lien Anh Ha, AU - Donato,Celeste, AU - Quan Toh,Zheng, AU - Higgins,Rachel, AU - Van de Sandt,Carolien, AU - Lemke,Melissa M, AU - Lee,Christina Y, AU - Shoffner,Suzanne K, AU - Flanagan,Katie L, AU - Arnold,Kelly B, AU - Mordant,Francesca L, AU - Mulholland,Kim, AU - Bines,Julie, AU - Dohle,Kate, AU - Pellicci,Daniel G, AU - Curtis,Nigel, AU - McNab,Sarah, AU - Steer,Andrew, AU - Saffery,Richard, AU - Subbarao,Kanta, AU - Chung,Amy W, AU - Kedzierska,Katherine, AU - Burgner,David P, AU - Crawford,Nigel W, Y1 - 2020/11/11/ PY - 2020/07/21/received PY - 2020/10/20/accepted PY - 2020/11/12/entrez PY - 2020/11/13/pubmed PY - 2020/11/25/medline SP - 5703 EP - 5703 JF - Nature communications JO - Nat Commun VL - 11 IS - 1 N2 - Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. Here, we describe clinical features, virology, longitudinal cellular, and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody responses in a family of two parents with PCR-confirmed symptomatic SARS-CoV-2 infection and their three children, who tested repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children are similar to their parents at all timepoints. All family members have salivary anti-SARS-CoV-2 antibodies detected, predominantly IgA, that coincide with symptom resolution in 3 of 4 symptomatic members. Plasma from both parents and one child have IgG antibody against the S1 protein and virus-neutralizing activity detected. Using a systems serology approach, we demonstrate higher levels of SARS-CoV-2-specific antibody features of these family members compared to healthy controls. These data indicate that children can mount an immune response to SARS-CoV-2 without virological confirmation of infection, raising the possibility that immunity in children can prevent the establishment of SARS-CoV-2 infection. Relying on routine virological and serological testing may not identify exposed children, with implications for epidemiological and clinical studies across the life-span. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/33177504/Immune_responses_to_SARS_CoV_2_in_three_children_of_parents_with_symptomatic_COVID_19_ L2 - https://doi.org/10.1038/s41467-020-19545-8 DB - PRIME DP - Unbound Medicine ER -