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Stretchable Electrode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction.
Anal Chem. 2020 12 01; 92(23):15639-15646.AC

Abstract

Vascular endothelial cells (ECs) are natively exposed to dynamic cyclic stretch and respond to it by the production of vasoactive molecules. Among them, reactive oxygen species (ROS) are closely implicated to the endothelial function and vascular homeostasis. However, the dynamic monitoring of ROS release during endothelial mechanotransduction remains a steep challenge. Herein, we developed a stretchable electrochemical sensor by decoration of uniform and ultrasmall platinum nanoparticles (Pt NPs) on gold nanotube (Au NT) networks (denoted as Au@Pt NTs). The orchestrated structure exhibited prominent electrocatalytic property toward the oxidation of hydrogen peroxide (H2O2) (as the most stable ROS) while maintaining excellent mechanical compliance of Au NT networks. Moreover, the favorable biocompatibility of Au NTs and Pt NPs promoted the adhesion and proliferation of ECs cultured thereon. These allowed in situ inducing ECs mechanotransduction and synchronously real-time monitoring of H2O2 release. Further investigation revealed that the production of H2O2 was positively correlated with the applied mechanical strains and could be boosted by other coexisting pathogenic factors. This indicates the great prospect of our proposed sensor in exploring ROS-related signaling for the deep understanding of cell mechanotransduction and vascular disorder.

Authors+Show Affiliations

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33179904

Citation

Fan, Wen-Ting, et al. "Stretchable Electrode Based On Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction." Analytical Chemistry, vol. 92, no. 23, 2020, pp. 15639-15646.
Fan WT, Qin Y, Hu XB, et al. Stretchable Electrode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction. Anal Chem. 2020;92(23):15639-15646.
Fan, W. T., Qin, Y., Hu, X. B., Yan, J., Wu, W. T., Liu, Y. L., & Huang, W. H. (2020). Stretchable Electrode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction. Analytical Chemistry, 92(23), 15639-15646. https://doi.org/10.1021/acs.analchem.0c04015
Fan WT, et al. Stretchable Electrode Based On Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction. Anal Chem. 2020 12 1;92(23):15639-15646. PubMed PMID: 33179904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stretchable Electrode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mechanotransduction. AU - Fan,Wen-Ting, AU - Qin,Yu, AU - Hu,Xue-Bo, AU - Yan,Jing, AU - Wu,Wen-Tao, AU - Liu,Yan-Ling, AU - Huang,Wei-Hua, Y1 - 2020/11/12/ PY - 2020/11/13/pubmed PY - 2021/2/24/medline PY - 2020/11/12/entrez SP - 15639 EP - 15646 JF - Analytical chemistry JO - Anal Chem VL - 92 IS - 23 N2 - Vascular endothelial cells (ECs) are natively exposed to dynamic cyclic stretch and respond to it by the production of vasoactive molecules. Among them, reactive oxygen species (ROS) are closely implicated to the endothelial function and vascular homeostasis. However, the dynamic monitoring of ROS release during endothelial mechanotransduction remains a steep challenge. Herein, we developed a stretchable electrochemical sensor by decoration of uniform and ultrasmall platinum nanoparticles (Pt NPs) on gold nanotube (Au NT) networks (denoted as Au@Pt NTs). The orchestrated structure exhibited prominent electrocatalytic property toward the oxidation of hydrogen peroxide (H2O2) (as the most stable ROS) while maintaining excellent mechanical compliance of Au NT networks. Moreover, the favorable biocompatibility of Au NTs and Pt NPs promoted the adhesion and proliferation of ECs cultured thereon. These allowed in situ inducing ECs mechanotransduction and synchronously real-time monitoring of H2O2 release. Further investigation revealed that the production of H2O2 was positively correlated with the applied mechanical strains and could be boosted by other coexisting pathogenic factors. This indicates the great prospect of our proposed sensor in exploring ROS-related signaling for the deep understanding of cell mechanotransduction and vascular disorder. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/33179904/Stretchable_Electrode_Based_on_Au@Pt_Nanotube_Networks_for_Real_Time_Monitoring_of_ROS_Signaling_in_Endothelial_Mechanotransduction_ L2 - https://doi.org/10.1021/acs.analchem.0c04015 DB - PRIME DP - Unbound Medicine ER -