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An integrated approach to reveal the chemical changes of Ligustri Lucidi Fructus during wine steaming processing.
J Pharm Biomed Anal. 2021 Jan 30; 193:113667.JP

Abstract

Ligustri Lucidi Fructus (LLF) is a traditional Chinese medicine to treat osteopathic and hepatic diseases. Wine steaming is the major processing method for LLF in Chinese Pharmacopoeia, but the chemical changes involved are still unclear. In this study, a research strategy was proposed to reveal the chemical changes during wine steaming processing of LLF. Firstly, in total 104 compounds were tentatively identified using UHPLC/Orbitrap-MS. Secondly, potential chemical changes were revealed by comparing the peak areas of the 104 compounds between LLF and the corresponding wine-steamed LLF (WLLF). The results indicated that iridoid and phenylethanoid esters were readily hydrolyzed during wine steaming processing, while organic acids, flavonoids, and triterpenes were stable. Finally, 7 selected compounds were simultaneously determined in 21 batches of LLF/WLLF samples using a 10-min UPLC/UV method. The contents of 4 esters decreased from 3.64% to 2.71%, and the contents of 3 potential hydrolysis products increased from 0.27% to 0.88% after processing. Our study indicated that hydrolysis of iridoid and phenylethanoid esters such as specneuzhenide was responsible for the chemical changes during the wine processing of LLF. The proposed strategy could be employed to investigate the chemical changes of other herbs during processing.

Authors+Show Affiliations

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Electronic address: yemin@bjmu.edu.cn.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Electronic address: qiaoxue@bjmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33181428

Citation

Shang, Zhanpeng, et al. "An Integrated Approach to Reveal the Chemical Changes of Ligustri Lucidi Fructus During Wine Steaming Processing." Journal of Pharmaceutical and Biomedical Analysis, vol. 193, 2021, p. 113667.
Shang Z, Xu L, Zhang Y, et al. An integrated approach to reveal the chemical changes of Ligustri Lucidi Fructus during wine steaming processing. J Pharm Biomed Anal. 2021;193:113667.
Shang, Z., Xu, L., Zhang, Y., Ye, M., & Qiao, X. (2021). An integrated approach to reveal the chemical changes of Ligustri Lucidi Fructus during wine steaming processing. Journal of Pharmaceutical and Biomedical Analysis, 193, 113667. https://doi.org/10.1016/j.jpba.2020.113667
Shang Z, et al. An Integrated Approach to Reveal the Chemical Changes of Ligustri Lucidi Fructus During Wine Steaming Processing. J Pharm Biomed Anal. 2021 Jan 30;193:113667. PubMed PMID: 33181428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An integrated approach to reveal the chemical changes of Ligustri Lucidi Fructus during wine steaming processing. AU - Shang,Zhanpeng, AU - Xu,Lulu, AU - Zhang,Yaqun, AU - Ye,Min, AU - Qiao,Xue, Y1 - 2020/10/08/ PY - 2020/04/08/received PY - 2020/09/21/revised PY - 2020/09/29/accepted PY - 2020/11/13/pubmed PY - 2021/6/22/medline PY - 2020/11/12/entrez KW - Chemical changes KW - Esters KW - Hydrolysis KW - Ligustri Lucidi Fructus (LLF) KW - Wine-steamed Ligustri Lucidi Fructus (WLLF) SP - 113667 EP - 113667 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 193 N2 - Ligustri Lucidi Fructus (LLF) is a traditional Chinese medicine to treat osteopathic and hepatic diseases. Wine steaming is the major processing method for LLF in Chinese Pharmacopoeia, but the chemical changes involved are still unclear. In this study, a research strategy was proposed to reveal the chemical changes during wine steaming processing of LLF. Firstly, in total 104 compounds were tentatively identified using UHPLC/Orbitrap-MS. Secondly, potential chemical changes were revealed by comparing the peak areas of the 104 compounds between LLF and the corresponding wine-steamed LLF (WLLF). The results indicated that iridoid and phenylethanoid esters were readily hydrolyzed during wine steaming processing, while organic acids, flavonoids, and triterpenes were stable. Finally, 7 selected compounds were simultaneously determined in 21 batches of LLF/WLLF samples using a 10-min UPLC/UV method. The contents of 4 esters decreased from 3.64% to 2.71%, and the contents of 3 potential hydrolysis products increased from 0.27% to 0.88% after processing. Our study indicated that hydrolysis of iridoid and phenylethanoid esters such as specneuzhenide was responsible for the chemical changes during the wine processing of LLF. The proposed strategy could be employed to investigate the chemical changes of other herbs during processing. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/33181428/An_integrated_approach_to_reveal_the_chemical_changes_of_Ligustri_Lucidi_Fructus_during_wine_steaming_processing_ DB - PRIME DP - Unbound Medicine ER -