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Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice.
Mol Plant. 2021 02 01; 14(2):253-266.MP

Abstract

Nucleotide-binding leucine-rich repeat (NLR) proteins play critical roles in plant immunity. However, how NLRs are regulated and activate defense signaling is not fully understood. The rice (Oryza sativa) NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10 (APIP10) negatively regulates Piz-t accumulation. In this study, we found that APIP10 interacts with two rice transcription factors, VASCULAR PLANT ONE-ZINC FINGER 1 (OsVOZ1) and OsVOZ2, and promotes their degradation through the 26S proteasome pathway. OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta. The osvoz1 and osvoz2 single mutants display modest but opposite M. oryzae resistance in the non-Piz-t background. However, the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death, and silencing of both genes via RNA interference also leads to dwarfism, mild cell death, and enhanced resistance to M. oryzae in the non-Piz-t background. Both OsVOZ1 and OsVOZ2 interact with Piz-t. Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription, reactive oxygen species-dependent cell death, and resistance to M. oryzae containing AvrPiz-t. Taken together, these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.

Authors+Show Affiliations

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA.Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: ningyuese@caas.cn.Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA. Electronic address: wang.620@osu.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33186754

Citation

Wang, Jiyang, et al. "Two VOZ Transcription Factors Link an E3 Ligase and an NLR Immune Receptor to Modulate Immunity in Rice." Molecular Plant, vol. 14, no. 2, 2021, pp. 253-266.
Wang J, Wang R, Fang H, et al. Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice. Mol Plant. 2021;14(2):253-266.
Wang, J., Wang, R., Fang, H., Zhang, C., Zhang, F., Hao, Z., You, X., Shi, X., Park, C. H., Hua, K., He, F., Bellizzi, M., Xuan Vo, K. T., Jeon, J. S., Ning, Y., & Wang, G. L. (2021). Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice. Molecular Plant, 14(2), 253-266. https://doi.org/10.1016/j.molp.2020.11.005
Wang J, et al. Two VOZ Transcription Factors Link an E3 Ligase and an NLR Immune Receptor to Modulate Immunity in Rice. Mol Plant. 2021 02 1;14(2):253-266. PubMed PMID: 33186754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice. AU - Wang,Jiyang, AU - Wang,Ruyi, AU - Fang,Hong, AU - Zhang,Chongyang, AU - Zhang,Fan, AU - Hao,Zeyun, AU - You,Xiaoman, AU - Shi,Xuetao, AU - Park,Chan Ho, AU - Hua,Kangyu, AU - He,Feng, AU - Bellizzi,Maria, AU - Xuan Vo,Kieu Thi, AU - Jeon,Jong-Seong, AU - Ning,Yuese, AU - Wang,Guo-Liang, Y1 - 2020/11/10/ PY - 2020/02/16/received PY - 2020/10/03/revised PY - 2020/11/08/accepted PY - 2020/11/14/pubmed PY - 2020/11/14/medline PY - 2020/11/13/entrez KW - E3 ligase KW - NLR protein KW - VOZ transcription factor KW - cell death KW - fungal pathogen Magnaporthe oryzae KW - rice SP - 253 EP - 266 JF - Molecular plant JO - Mol Plant VL - 14 IS - 2 N2 - Nucleotide-binding leucine-rich repeat (NLR) proteins play critical roles in plant immunity. However, how NLRs are regulated and activate defense signaling is not fully understood. The rice (Oryza sativa) NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10 (APIP10) negatively regulates Piz-t accumulation. In this study, we found that APIP10 interacts with two rice transcription factors, VASCULAR PLANT ONE-ZINC FINGER 1 (OsVOZ1) and OsVOZ2, and promotes their degradation through the 26S proteasome pathway. OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta. The osvoz1 and osvoz2 single mutants display modest but opposite M. oryzae resistance in the non-Piz-t background. However, the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death, and silencing of both genes via RNA interference also leads to dwarfism, mild cell death, and enhanced resistance to M. oryzae in the non-Piz-t background. Both OsVOZ1 and OsVOZ2 interact with Piz-t. Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription, reactive oxygen species-dependent cell death, and resistance to M. oryzae containing AvrPiz-t. Taken together, these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. SN - 1752-9867 UR - https://www.unboundmedicine.com/medline/citation/33186754/Two_VOZ_transcription_factors_link_an_E3_ligase_and_an_NLR_immune_receptor_to_modulate_immunity_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1674-2052(20)30384-1 DB - PRIME DP - Unbound Medicine ER -
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