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An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae.
Plant J. 2021 02; 105(4):978-993.PJ

Abstract

A chromosome-specific painting technique has been developed which combines the most recent approaches of the companion disciplines of molecular cytogenetics and genome research. We developed seven oligonucleotide (oligo) pools derivd from single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these then produced stable fluorescence in situ hybridization (FISH) signals on chromosomes of each linkage group of wheat and barley. The pooled oligo probes were applied to high-throughput karyotyping of the chromosomes of other Triticeae species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study also extended to some wheat-alien amphiploids and derived lines. We demonstrated that a complete set of whole-chromosome oligo painting probes facilitated the study of inter-species chromosome homologous relationships and visualized non-homologous chromosomal rearrangements in Triticeae species and some wheat-alien species derivatives. When combined with other non-denaturing FISH procedures using tandem-repeat oligos, the newly developed oligo painting techniques provide an efficient tool for the study of chromosome structure, organization, and evolution among any wild Triticeae species with non-sequenced genomes.

Authors+Show Affiliations

Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu, 611731, China.Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Agricultural College of Yangzhou University, Yangzhou, 225009, China. Key Laboratory of Plant Functional Genomics of the Ministry of Education/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu, 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu, 611731, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu, 611731, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33210785

Citation

Li, Guangrong, et al. "An Efficient Oligo-FISH Painting System for Revealing Chromosome Rearrangements and Polyploidization in Triticeae." The Plant Journal : for Cell and Molecular Biology, vol. 105, no. 4, 2021, pp. 978-993.
Li G, Zhang T, Yu Z, et al. An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae. Plant J. 2021;105(4):978-993.
Li, G., Zhang, T., Yu, Z., Wang, H., Yang, E., & Yang, Z. (2021). An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae. The Plant Journal : for Cell and Molecular Biology, 105(4), 978-993. https://doi.org/10.1111/tpj.15081
Li G, et al. An Efficient Oligo-FISH Painting System for Revealing Chromosome Rearrangements and Polyploidization in Triticeae. Plant J. 2021;105(4):978-993. PubMed PMID: 33210785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae. AU - Li,Guangrong, AU - Zhang,Tao, AU - Yu,Zhihui, AU - Wang,Hongjin, AU - Yang,Ennian, AU - Yang,Zujun, Y1 - 2020/12/13/ PY - 2020/09/03/received PY - 2020/10/25/revised PY - 2020/11/09/accepted PY - 2020/11/20/pubmed PY - 2020/11/20/medline PY - 2020/11/19/entrez KW - Oligo-FISH KW - Triticeae species KW - chromosome rearrangement KW - wheat-barley synteny SP - 978 EP - 993 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 105 IS - 4 N2 - A chromosome-specific painting technique has been developed which combines the most recent approaches of the companion disciplines of molecular cytogenetics and genome research. We developed seven oligonucleotide (oligo) pools derivd from single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these then produced stable fluorescence in situ hybridization (FISH) signals on chromosomes of each linkage group of wheat and barley. The pooled oligo probes were applied to high-throughput karyotyping of the chromosomes of other Triticeae species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study also extended to some wheat-alien amphiploids and derived lines. We demonstrated that a complete set of whole-chromosome oligo painting probes facilitated the study of inter-species chromosome homologous relationships and visualized non-homologous chromosomal rearrangements in Triticeae species and some wheat-alien species derivatives. When combined with other non-denaturing FISH procedures using tandem-repeat oligos, the newly developed oligo painting techniques provide an efficient tool for the study of chromosome structure, organization, and evolution among any wild Triticeae species with non-sequenced genomes. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/33210785/An_efficient_Oligo_FISH_painting_system_for_revealing_chromosome_rearrangements_and_polyploidization_in_Triticeae_ L2 - https://doi.org/10.1111/tpj.15081 DB - PRIME DP - Unbound Medicine ER -