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Mosquito-infecting virus Espirito Santo virus inhibits replication and spread of dengue virus.
J Med Virol. 2021 06; 93(6):3362-3373.JM

Abstract

The primary vector of dengue virus (DENV) is Aedes aegypti. The mosquito-infecting virus, Espirito Santo virus (ESV), does not infect Vero (mammalian) cells and grows in mosquito (C6/36) cells without cytopathic effects. Effects of ESV infection on replication of DENV were explored in vitro and in vivo, analyzing protein, RNA genome expression, and plaque formation. ESV and DENV simultaneous coinfection did not block protein synthesis from either virus but did result in inhibition of DENV replication in mosquito cells. Furthermore, ESV superinfected with DENV resulted in inhibition of DENV replication and spread in A. aegypti, thus reducing vector competence. Tissue culture experiments on viral kinetics of ESV and DENV coinfection showed that neither virus significantly affects the replication of the other in Vero, HeLa, or HEK cells. Hence, ESV blocks DENV replication in insect cells, but not the mammalian cells evaluated here. Our study provides new insights into ESV-induced suppression of DENV, a globally important pathogen impacting public health.

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Publisher Full Text (DOI)

Authors+Show Affiliations

White AV
Department of Health Education and Promotion, Environmental Health Sciences Program, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA.
Fan M
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA.
Mazzara JM
Department of Health Education and Promotion, Environmental Health Sciences Program, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA.
Roper RL
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, USA.
Richards SL
Department of Health Education and Promotion, Environmental Health Sciences Program, College of Health and Human Performance, East Carolina University, Greenville, North Carolina, USA.

MeSH

AedesAnimalsBirnaviridaeChlorocebus aethiopsCoinfectionDengueDengue VirusHEK293 CellsHeLa CellsHumansMosquito VectorsVero CellsVirus Replication

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33219544

Citation

White, Avian V., et al. "Mosquito-infecting Virus Espirito Santo Virus Inhibits Replication and Spread of Dengue Virus." Journal of Medical Virology, vol. 93, no. 6, 2021, pp. 3362-3373.
White AV, Fan M, Mazzara JM, et al. Mosquito-infecting virus Espirito Santo virus inhibits replication and spread of dengue virus. J Med Virol. 2021;93(6):3362-3373.
White, A. V., Fan, M., Mazzara, J. M., Roper, R. L., & Richards, S. L. (2021). Mosquito-infecting virus Espirito Santo virus inhibits replication and spread of dengue virus. Journal of Medical Virology, 93(6), 3362-3373. https://doi.org/10.1002/jmv.26686
White AV, et al. Mosquito-infecting Virus Espirito Santo Virus Inhibits Replication and Spread of Dengue Virus. J Med Virol. 2021;93(6):3362-3373. PubMed PMID: 33219544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mosquito-infecting virus Espirito Santo virus inhibits replication and spread of dengue virus. AU - White,Avian V, AU - Fan,Ming, AU - Mazzara,Jordan M, AU - Roper,Rachel L, AU - Richards,Stephanie L, Y1 - 2020/12/01/ PY - 2020/10/31/revised PY - 2019/11/22/received PY - 2020/11/18/accepted PY - 2020/11/22/pubmed PY - 2021/10/9/medline PY - 2020/11/21/entrez KW - Aedes aegypti KW - dengue KW - insect-specific virus (ISV) KW - mosquito-infecting virus KW - mosquito-specific virus KW - superinfection KW - vector competence SP - 3362 EP - 3373 JF - Journal of medical virology JO - J Med Virol VL - 93 IS - 6 N2 - The primary vector of dengue virus (DENV) is Aedes aegypti. The mosquito-infecting virus, Espirito Santo virus (ESV), does not infect Vero (mammalian) cells and grows in mosquito (C6/36) cells without cytopathic effects. Effects of ESV infection on replication of DENV were explored in vitro and in vivo, analyzing protein, RNA genome expression, and plaque formation. ESV and DENV simultaneous coinfection did not block protein synthesis from either virus but did result in inhibition of DENV replication in mosquito cells. Furthermore, ESV superinfected with DENV resulted in inhibition of DENV replication and spread in A. aegypti, thus reducing vector competence. Tissue culture experiments on viral kinetics of ESV and DENV coinfection showed that neither virus significantly affects the replication of the other in Vero, HeLa, or HEK cells. Hence, ESV blocks DENV replication in insect cells, but not the mammalian cells evaluated here. Our study provides new insights into ESV-induced suppression of DENV, a globally important pathogen impacting public health. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/33219544/Mosquito_infecting_virus_Espirito_Santo_virus_inhibits_replication_and_spread_of_dengue_virus_ DB - PRIME DP - Unbound Medicine ER -