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Protection against acute cerebral ischemia/reperfusion injury by Leonuri Herba Total Alkali via modulation of BDNF-TrKB-PI3K/Akt signaling pathway in rats.
Biomed Pharmacother. 2021 Jan; 133:111021.BP

Abstract

OBJECTIVE

To observe the brain protective effect of Leonuri Herba Total Alkali (LHA) on cerebral ischemia reperfusion injury in rats, so as to provide basis for clinical research.

METHODS

Adult male SD rats were randomly assigned into sham group, middle cerebral artery occlusion/reperfusion (MCAO/R) group, and LHA + MCAO/R group (25 mg/kg, 50 mg/kg, and 100 mg/kg). Fourteen days before MCAO/R surgery, the rats in treatment groups were orally administered with LHA in ultrapure water once daily for 14 days, while rats in the sham and MCAO groups were given the same amount of saline in advance. After 1 h of administration on the 14th day, MCAO surgery was subjected. The neurological deficits, brain infarct volume, histopathology, immunofluorescence, inflammation indicators and the gene/protein expressions of BDNF-TrKB-PI3K/Akt signaling pathway in the rat brain tissue were evaluated 24 h after the MCAO/R-injury.

RESULTS

It was found that rats in LHA pre-administration group showed significantly reduced neurological deficit scores, infarction volume, the serum levels of NSE and S100β. Meanwhile, the content of Evans Blue (EB) in brain tissue from LHA group was decreased, as well as the levels of inflammatory cytokines and their gene levels. Moreover, LHA pre-administration inhibited the expression of CD44, GFAP, FOXO1 and promoted the expression of BDNF and NeuN. In addition, LHA pre-administration could up-regulate the protein expression of TrkB, p-PI3K, p-Akt, Bcl-2, and down-regulate the protein expression of Bax, and increase the level of Bcl-2/Bax.

CONCLUSIONS

The study demonstrated that LHA pre-administration could regulate the PI3K/Akt pathway by increasing BDNF levels, and play a neuroprotective role in cerebral ischemia-reperfusion injury.

Authors+Show Affiliations

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.National International Cooperation Base of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: jinxin.miao@yahoo.com.National International Cooperation Base of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: miaomingsan@hactcm.edu.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33227709

Citation

Li, Yan, et al. "Protection Against Acute Cerebral Ischemia/reperfusion Injury By Leonuri Herba Total Alkali Via Modulation of BDNF-TrKB-PI3K/Akt Signaling Pathway in Rats." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 133, 2021, p. 111021.
Li Y, Xiang L, Wang C, et al. Protection against acute cerebral ischemia/reperfusion injury by Leonuri Herba Total Alkali via modulation of BDNF-TrKB-PI3K/Akt signaling pathway in rats. Biomed Pharmacother. 2021;133:111021.
Li, Y., Xiang, L., Wang, C., Song, Y., Miao, J., & Miao, M. (2021). Protection against acute cerebral ischemia/reperfusion injury by Leonuri Herba Total Alkali via modulation of BDNF-TrKB-PI3K/Akt signaling pathway in rats. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 133, 111021. https://doi.org/10.1016/j.biopha.2020.111021
Li Y, et al. Protection Against Acute Cerebral Ischemia/reperfusion Injury By Leonuri Herba Total Alkali Via Modulation of BDNF-TrKB-PI3K/Akt Signaling Pathway in Rats. Biomed Pharmacother. 2021;133:111021. PubMed PMID: 33227709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against acute cerebral ischemia/reperfusion injury by Leonuri Herba Total Alkali via modulation of BDNF-TrKB-PI3K/Akt signaling pathway in rats. AU - Li,Yan, AU - Xiang,Liling, AU - Wang,Can, AU - Song,Yagang, AU - Miao,Jinxin, AU - Miao,Mingsan, Y1 - 2020/11/20/ PY - 2020/09/22/received PY - 2020/11/09/revised PY - 2020/11/15/accepted PY - 2020/11/24/pubmed PY - 2021/2/25/medline PY - 2020/11/23/entrez KW - BDNF KW - Cerebral ischemia KW - Inflammatory cytokines KW - Leonuri Herba Total Alkali KW - PI3K/Akt pathway SP - 111021 EP - 111021 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 133 N2 - OBJECTIVE: To observe the brain protective effect of Leonuri Herba Total Alkali (LHA) on cerebral ischemia reperfusion injury in rats, so as to provide basis for clinical research. METHODS: Adult male SD rats were randomly assigned into sham group, middle cerebral artery occlusion/reperfusion (MCAO/R) group, and LHA + MCAO/R group (25 mg/kg, 50 mg/kg, and 100 mg/kg). Fourteen days before MCAO/R surgery, the rats in treatment groups were orally administered with LHA in ultrapure water once daily for 14 days, while rats in the sham and MCAO groups were given the same amount of saline in advance. After 1 h of administration on the 14th day, MCAO surgery was subjected. The neurological deficits, brain infarct volume, histopathology, immunofluorescence, inflammation indicators and the gene/protein expressions of BDNF-TrKB-PI3K/Akt signaling pathway in the rat brain tissue were evaluated 24 h after the MCAO/R-injury. RESULTS: It was found that rats in LHA pre-administration group showed significantly reduced neurological deficit scores, infarction volume, the serum levels of NSE and S100β. Meanwhile, the content of Evans Blue (EB) in brain tissue from LHA group was decreased, as well as the levels of inflammatory cytokines and their gene levels. Moreover, LHA pre-administration inhibited the expression of CD44, GFAP, FOXO1 and promoted the expression of BDNF and NeuN. In addition, LHA pre-administration could up-regulate the protein expression of TrkB, p-PI3K, p-Akt, Bcl-2, and down-regulate the protein expression of Bax, and increase the level of Bcl-2/Bax. CONCLUSIONS: The study demonstrated that LHA pre-administration could regulate the PI3K/Akt pathway by increasing BDNF levels, and play a neuroprotective role in cerebral ischemia-reperfusion injury. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/33227709/Protection_against_acute_cerebral_ischemia/reperfusion_injury_by_Leonuri_Herba_Total_Alkali_via_modulation_of_BDNF_TrKB_PI3K/Akt_signaling_pathway_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(20)31213-0 DB - PRIME DP - Unbound Medicine ER -