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Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes.
EMBO J. 1987 Dec 01; 6(12):3833-9.EJ

Abstract

In experiments involving deletion and rearrangement of intron sequences two small regions of the intron in the yeast CYH2 ribosomal protein gene were found to play important roles in splicing of the pre-mRNA. One element lies downstream of the 5' splice site, and the other is upstream of the branchpoint sequence UACUAAC. Deletion of the element upstream of the branchpoint prevents spliceosome formation and blocks splicing in vivo and in vitro. Deletion of the element downstream of the 5' splice site does not on its own block splicing but rescues spliceosome formation and splicing of pre-mRNA lacking the element upstream of the branchpoint. These elements correspond to two regions of sequence complementarity which are a conserved feature of the introns in yeast pre-mRNAs. Mixing and matching of the elements from the ACT1 and CYH2 gene introns showed that these elements can cooperate in an intron-specific fashion to control spliceosome assembly.

Authors+Show Affiliations

MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3322814

Citation

Newman, A. "Specific Accessory Sequences in Saccharomyces Cerevisiae Introns Control Assembly of pre-mRNAs Into Spliceosomes." The EMBO Journal, vol. 6, no. 12, 1987, pp. 3833-9.
Newman A. Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes. EMBO J. 1987;6(12):3833-9.
Newman, A. (1987). Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes. The EMBO Journal, 6(12), 3833-9.
Newman A. Specific Accessory Sequences in Saccharomyces Cerevisiae Introns Control Assembly of pre-mRNAs Into Spliceosomes. EMBO J. 1987 Dec 1;6(12):3833-9. PubMed PMID: 3322814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes. A1 - Newman,A, PY - 1987/12/1/pubmed PY - 1987/12/1/medline PY - 1987/12/1/entrez SP - 3833 EP - 9 JF - The EMBO journal JO - EMBO J VL - 6 IS - 12 N2 - In experiments involving deletion and rearrangement of intron sequences two small regions of the intron in the yeast CYH2 ribosomal protein gene were found to play important roles in splicing of the pre-mRNA. One element lies downstream of the 5' splice site, and the other is upstream of the branchpoint sequence UACUAAC. Deletion of the element upstream of the branchpoint prevents spliceosome formation and blocks splicing in vivo and in vitro. Deletion of the element downstream of the 5' splice site does not on its own block splicing but rescues spliceosome formation and splicing of pre-mRNA lacking the element upstream of the branchpoint. These elements correspond to two regions of sequence complementarity which are a conserved feature of the introns in yeast pre-mRNAs. Mixing and matching of the elements from the ACT1 and CYH2 gene introns showed that these elements can cooperate in an intron-specific fashion to control spliceosome assembly. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/3322814/Specific_accessory_sequences_in_Saccharomyces_cerevisiae_introns_control_assembly_of_pre_mRNAs_into_spliceosomes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1987&volume=6&issue=12&spage=3833 DB - PRIME DP - Unbound Medicine ER -