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Therapeutic propensities, phytochemical composition, and toxicological evaluation of Anagallis arvensis (L.): A wild edible medicinal food plant.
Food Res Int. 2020 11; 137:109651.FR

Abstract

Anagallis arvensis (L.) is a wild edible food plant that has been used in folklore as a natural remedy for treating common ailments. This study aimed to explore the biochemical properties and toxicity of methanol (MeOH) and dichloromethane (DCM) extracts of A. arvensis (aerial and root parts). Bioactive contents were assessed spectrophotometrically, and the secondary metabolites were identified by UHPLC-MS analysis. DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelating assays were employed to assess antioxidant activity. Inhibitory potential against key enzymes (α-glucosidase, urease, lipoxygenase (LOX), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE)) were also assessed. MTT assay was employed to test toxicity against SW-480, MDA-MB-231, CaSki, MCF-7, and DU-145 cancer cell lines. Methanolic extracts showed highest phenolic (aerial-MeOH: 27.5 mg GAE/g extract; root-MeOH: 21.17 mg GAE/g extract) and flavonoid (aerial-MeOH: 26.15 mg QE/g extract; root-MeOH: 19.07 mg QE/g extract) contents, and potent antioxidant activities. The aerial-MeOH extract was most potent for DPPH (IC50: 231 ug/mL), ABTS (131.12 mg TE/g extract), FRAP (82.97 mg TE/g extract), and CUPRAC (137.15 mg TE/g extract) antioxidant assays. All extracts were cytotoxic towards tested cancer cells with IC50 values ranging from 12.57 to 294.5 µg/mL and conferred a comparatively strong inhibition against α-glucosidase (aerial-DCM extract showed the highest inhibition against α-glucosidase with IC50 value of 20.97 µg /mL), while aerial extracts were also considerably active against BChE (aerial-MeOH IC50: 224.63 µg /mL), LOX (aerial-DCM IC50: 385.7 µg /mL). Likewise, aerial-MeOH extract was most active against urease enzyme (IC50: 129.72 µg /mL). UHPLC-MS investigation of methanolic extracts showed the existence of important phenolics, flavonoids, and saponins, including methyl gallte, quercetin, lanceoletin, and balanitesin, amongst others. Moreover, principal component analysis (PCA) highlighted the correlation amongst bioactive contents and observed biological activities. A. arvensis extracts could be regarded as a natural source of bioactive antioxidants, enzyme inhibitors and anticancer agents and can be further investigated as a lead source for food and pharmaceutical products. However, further studies to isolate, purify, and to characterize its bioactive phytochemicals are needed.

Authors+Show Affiliations

School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway Selangor, Darul Ehsan, Malaysia; Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore 54000, Pakistan.Department of Biology, Faculty of Science, Selcuk University, Campus/Konya, Turkey.Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway Selangor, Darul Ehsan, Malaysia.Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway Selangor, Darul Ehsan, Malaysia.Institute of Research and Development, Duy Tan University, Da Nang 550000, Viet Nam; Department of Health Sciences, Faculty of Science, University of Mauritius, Mauritius.School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway Selangor, Darul Ehsan, Malaysia; Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway Selangor, Darul Ehsan, Malaysia; Global Asia in 21(st) Century Research Platform, Monash University, Jalan Lagoon Selatan, Bandar Sunway 47500, Petaling Jaya, Selangor, Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33233230

Citation

Saleem, Hammad, et al. "Therapeutic Propensities, Phytochemical Composition, and Toxicological Evaluation of Anagallis Arvensis (L.): a Wild Edible Medicinal Food Plant." Food Research International (Ottawa, Ont.), vol. 137, 2020, p. 109651.
Saleem H, Zengin G, Ahmad I, et al. Therapeutic propensities, phytochemical composition, and toxicological evaluation of Anagallis arvensis (L.): A wild edible medicinal food plant. Food Res Int. 2020;137:109651.
Saleem, H., Zengin, G., Ahmad, I., Htar, T. T., Naidu, R., Mahomoodally, M. F., & Ahemad, N. (2020). Therapeutic propensities, phytochemical composition, and toxicological evaluation of Anagallis arvensis (L.): A wild edible medicinal food plant. Food Research International (Ottawa, Ont.), 137, 109651. https://doi.org/10.1016/j.foodres.2020.109651
Saleem H, et al. Therapeutic Propensities, Phytochemical Composition, and Toxicological Evaluation of Anagallis Arvensis (L.): a Wild Edible Medicinal Food Plant. Food Res Int. 2020;137:109651. PubMed PMID: 33233230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic propensities, phytochemical composition, and toxicological evaluation of Anagallis arvensis (L.): A wild edible medicinal food plant. AU - Saleem,Hammad, AU - Zengin,Gokhan, AU - Ahmad,Irshad, AU - Htar,Thet Thet, AU - Naidu,Rakesh, AU - Mahomoodally,Mohamad Fawzi, AU - Ahemad,Nafees, Y1 - 2020/09/09/ PY - 2020/02/11/received PY - 2020/05/11/revised PY - 2020/08/28/accepted PY - 2020/11/25/entrez PY - 2020/11/26/pubmed PY - 2021/5/15/medline KW - Anagallis arvensis KW - Antioxidant KW - Cytotoxicity KW - Enzyme inhibition KW - Medicinal plant KW - Phytochemicals KW - UHPLC-MS SP - 109651 EP - 109651 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 137 N2 - Anagallis arvensis (L.) is a wild edible food plant that has been used in folklore as a natural remedy for treating common ailments. This study aimed to explore the biochemical properties and toxicity of methanol (MeOH) and dichloromethane (DCM) extracts of A. arvensis (aerial and root parts). Bioactive contents were assessed spectrophotometrically, and the secondary metabolites were identified by UHPLC-MS analysis. DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelating assays were employed to assess antioxidant activity. Inhibitory potential against key enzymes (α-glucosidase, urease, lipoxygenase (LOX), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE)) were also assessed. MTT assay was employed to test toxicity against SW-480, MDA-MB-231, CaSki, MCF-7, and DU-145 cancer cell lines. Methanolic extracts showed highest phenolic (aerial-MeOH: 27.5 mg GAE/g extract; root-MeOH: 21.17 mg GAE/g extract) and flavonoid (aerial-MeOH: 26.15 mg QE/g extract; root-MeOH: 19.07 mg QE/g extract) contents, and potent antioxidant activities. The aerial-MeOH extract was most potent for DPPH (IC50: 231 ug/mL), ABTS (131.12 mg TE/g extract), FRAP (82.97 mg TE/g extract), and CUPRAC (137.15 mg TE/g extract) antioxidant assays. All extracts were cytotoxic towards tested cancer cells with IC50 values ranging from 12.57 to 294.5 µg/mL and conferred a comparatively strong inhibition against α-glucosidase (aerial-DCM extract showed the highest inhibition against α-glucosidase with IC50 value of 20.97 µg /mL), while aerial extracts were also considerably active against BChE (aerial-MeOH IC50: 224.63 µg /mL), LOX (aerial-DCM IC50: 385.7 µg /mL). Likewise, aerial-MeOH extract was most active against urease enzyme (IC50: 129.72 µg /mL). UHPLC-MS investigation of methanolic extracts showed the existence of important phenolics, flavonoids, and saponins, including methyl gallte, quercetin, lanceoletin, and balanitesin, amongst others. Moreover, principal component analysis (PCA) highlighted the correlation amongst bioactive contents and observed biological activities. A. arvensis extracts could be regarded as a natural source of bioactive antioxidants, enzyme inhibitors and anticancer agents and can be further investigated as a lead source for food and pharmaceutical products. However, further studies to isolate, purify, and to characterize its bioactive phytochemicals are needed. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/33233230/Therapeutic_propensities_phytochemical_composition_and_toxicological_evaluation_of_Anagallis_arvensis__L__:_A_wild_edible_medicinal_food_plant_ DB - PRIME DP - Unbound Medicine ER -