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Inhibitory evaluation of Curculigo latifolia on α-glucosidase, DPP (IV) and in vitro studies in antidiabetic with molecular docking relevance to type 2 diabetes mellitus.
J Enzyme Inhib Med Chem. 2021 Dec; 36(1):109-121.JE

Abstract

The inhibition of α-glucosidase and DPP enzymes capable of effectively reducing blood glucose level in the management of type 2 diabetes. The purpose of the present study is to evaluate the inhibitory potential of α-glucosidase and DPP (IV) activity including with the 2-NBDG uptake assay and insulin secretion activities through in vitro studies. The selected of active compounds obtained from the screening of compounds by LC-MS were docked with the targeted enzyme that involved in the mechanism of T2DM. From the results, root extracts displayed a better promising outcome in α-glucosidase (IC50 2.72 ± 0.32) as compared with the fruit extracts (IC50 3.87 ± 0.32). Besides, root extracts also displayed a better activity in the inhibition of DPP (IV), enhance insulin secretion and glucose uptake activity. Molecular docking results revealing that phlorizin binds strongly with α-glucosidase, DPP (IV) and Insulin receptor (IR) enzymes with achieving the lowest binding energy value. The present work suggests several of the compounds have the potential that contribute towards inhibiting α-glucosidase and DPP (IV) and thus effective in lowering post-prandial hyperglycaemia.

Authors+Show Affiliations

Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Centre of Foundation Studies for Agricultural Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Department of Microbiology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Centre of Foundation Studies for Agricultural Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.Centre of Foundation Studies for Agricultural Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33249946

Citation

Zabidi, Nur Athirah, et al. "Inhibitory Evaluation of Curculigo Latifolia On Α-glucosidase, DPP (IV) and in Vitro Studies in Antidiabetic With Molecular Docking Relevance to Type 2 Diabetes Mellitus." Journal of Enzyme Inhibition and Medicinal Chemistry, vol. 36, no. 1, 2021, pp. 109-121.
Zabidi NA, Ishak NA, Hamid M, et al. Inhibitory evaluation of Curculigo latifolia on α-glucosidase, DPP (IV) and in vitro studies in antidiabetic with molecular docking relevance to type 2 diabetes mellitus. J Enzyme Inhib Med Chem. 2021;36(1):109-121.
Zabidi, N. A., Ishak, N. A., Hamid, M., Ashari, S. E., & Mohammad Latif, M. A. (2021). Inhibitory evaluation of Curculigo latifolia on α-glucosidase, DPP (IV) and in vitro studies in antidiabetic with molecular docking relevance to type 2 diabetes mellitus. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1), 109-121. https://doi.org/10.1080/14756366.2020.1844680
Zabidi NA, et al. Inhibitory Evaluation of Curculigo Latifolia On Α-glucosidase, DPP (IV) and in Vitro Studies in Antidiabetic With Molecular Docking Relevance to Type 2 Diabetes Mellitus. J Enzyme Inhib Med Chem. 2021;36(1):109-121. PubMed PMID: 33249946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory evaluation of Curculigo latifolia on α-glucosidase, DPP (IV) and in vitro studies in antidiabetic with molecular docking relevance to type 2 diabetes mellitus. AU - Zabidi,Nur Athirah, AU - Ishak,Nur Akmal, AU - Hamid,Muhajir, AU - Ashari,Siti Efliza, AU - Mohammad Latif,Muhammad Alif, PY - 2020/11/30/entrez PY - 2020/12/1/pubmed PY - 2021/6/25/medline KW - Curculigo latifolia KW - DPP (IV) KW - molecular docking KW - type 2 diabetes KW - α-glucosidase SP - 109 EP - 121 JF - Journal of enzyme inhibition and medicinal chemistry JO - J Enzyme Inhib Med Chem VL - 36 IS - 1 N2 - The inhibition of α-glucosidase and DPP enzymes capable of effectively reducing blood glucose level in the management of type 2 diabetes. The purpose of the present study is to evaluate the inhibitory potential of α-glucosidase and DPP (IV) activity including with the 2-NBDG uptake assay and insulin secretion activities through in vitro studies. The selected of active compounds obtained from the screening of compounds by LC-MS were docked with the targeted enzyme that involved in the mechanism of T2DM. From the results, root extracts displayed a better promising outcome in α-glucosidase (IC50 2.72 ± 0.32) as compared with the fruit extracts (IC50 3.87 ± 0.32). Besides, root extracts also displayed a better activity in the inhibition of DPP (IV), enhance insulin secretion and glucose uptake activity. Molecular docking results revealing that phlorizin binds strongly with α-glucosidase, DPP (IV) and Insulin receptor (IR) enzymes with achieving the lowest binding energy value. The present work suggests several of the compounds have the potential that contribute towards inhibiting α-glucosidase and DPP (IV) and thus effective in lowering post-prandial hyperglycaemia. SN - 1475-6374 UR - https://www.unboundmedicine.com/medline/citation/33249946/Inhibitory_evaluation_of_Curculigo_latifolia_on_α_glucosidase_DPP__IV__and_in_vitro_studies_in_antidiabetic_with_molecular_docking_relevance_to_type_2_diabetes_mellitus_ L2 - https://www.tandfonline.com/doi/full/10.1080/14756366.2020.1844680 DB - PRIME DP - Unbound Medicine ER -