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Repeated exposure of Caco-2 versus Caco-2/HT29-MTX intestinal cell models to (nano)silver in vitro: Comparison of two commercially available colloidal silver products.
Sci Total Environ. 2021 Feb 01; 754:142324.ST

Abstract

Colloidal silver products are sold for a wide range of disinfectant and health applications. This has increased the potential for human exposure to silver nanoparticles (AgNPs) and ions (Ag+), for which oral ingestion is considered to be a major route of exposure. Our objective was to evaluate and compare the toxicity of two commercially available colloidal silver products on two human intestinal epithelial models under realistic exposure conditions. Mesosilver™ and AgC were characterized and a concentration range between 0.1 and 12 μg/mL chosen. Caco-2 cells vs. co-culture of Caco-2 and mucus-secreting HT29-MTX cells (90/10) were used. Repeated exposure was carried out to determine cell viability over 18 days of cell differentiation in 24-well plates. Selected concentrations (0.1, 1, and 3 μg/mL) were tested on cells cultured in E-plates and Transwells with the same repeated exposure regimen, to determine cell impedance, and cell viability and trans-epithelial electrical resistance (TEER), respectively. Silver uptake, intracellular localisation, and translocation were determined by CytoViva™, HIM-SIMS, and ICP-MS. Genotoxicity was determined on acutely-exposed proliferating Caco-2 cells by γH2AX immunofluorescence staining. Repeated exposure of a given concentration of AgC, which is composed solely of ionic silver, generally exerted more toxic effects on Caco-2 cells than Mesosilver™, which contains a mix of AgNPs and ionic silver. Due to its patchy structure, the presence of mucus in the Caco-2/HT29-MTX co-culture only slightly mitigated the deleterious effects on cell viability. Increased genotoxicity was observed for AgC on proliferating Caco-2 cells. Silver uptake, intracellular localisation, and translocation were similar. In conclusion, Mesosilver™ and AgC colloidal silver products show different levels of gut toxicity due to the forms of distinct silver (AgNPs and/or Ag+) contained within. This study highlights the applicability of high-resolution (chemical) imaging to detect and localize silver and provides insights into its uptake mechanisms, intracellular fate and cellular effects.

Authors+Show Affiliations

Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Luxembourg Institute of Science and Technology (LIST), 41, rue de Brill, Belvaux L-4422, Luxembourg.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Centre de Microscopie Électronique Appliquée à la Biologie, CMEAB, 133 route de Narbonne, 31062 Toulouse, France.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Luxembourg Institute of Science and Technology (LIST), 41, rue de Brill, Belvaux L-4422, Luxembourg.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Université de Nantes, CNRS, Institut des Matériaux Jean Rouxel, IMN, F-44000 Nantes, France.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Luxembourg Institute of Science and Technology (LIST), 41, rue de Brill, Belvaux L-4422, Luxembourg.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.INRAE, UR BIA, F-44316 Nantes, France.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France.Toxalim, Université de Toulouse, INRAE, INP-ENVT, INP-EI-Purpan, Université de Toulouse 3 Paul Sabatier, Toulouse, France. Electronic address: muriel.mercier-bonin@inrae.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33254900

Citation

Gillois, Kévin, et al. "Repeated Exposure of Caco-2 Versus Caco-2/HT29-MTX Intestinal Cell Models to (nano)silver in Vitro: Comparison of Two Commercially Available Colloidal Silver Products." The Science of the Total Environment, vol. 754, 2021, p. 142324.
Gillois K, Stoffels C, Leveque M, et al. Repeated exposure of Caco-2 versus Caco-2/HT29-MTX intestinal cell models to (nano)silver in vitro: Comparison of two commercially available colloidal silver products. Sci Total Environ. 2021;754:142324.
Gillois, K., Stoffels, C., Leveque, M., Fourquaux, I., Blesson, J., Mils, V., Cambier, S., Vignard, J., Terrisse, H., Mirey, G., Audinot, J. N., Theodorou, V., Ropers, M. H., Robert, H., & Mercier-Bonin, M. (2021). Repeated exposure of Caco-2 versus Caco-2/HT29-MTX intestinal cell models to (nano)silver in vitro: Comparison of two commercially available colloidal silver products. The Science of the Total Environment, 754, 142324. https://doi.org/10.1016/j.scitotenv.2020.142324
Gillois K, et al. Repeated Exposure of Caco-2 Versus Caco-2/HT29-MTX Intestinal Cell Models to (nano)silver in Vitro: Comparison of Two Commercially Available Colloidal Silver Products. Sci Total Environ. 2021 Feb 1;754:142324. PubMed PMID: 33254900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Repeated exposure of Caco-2 versus Caco-2/HT29-MTX intestinal cell models to (nano)silver in vitro: Comparison of two commercially available colloidal silver products. AU - Gillois,Kévin, AU - Stoffels,Charlotte, AU - Leveque,Mathilde, AU - Fourquaux,Isabelle, AU - Blesson,Justine, AU - Mils,Valérie, AU - Cambier,Sébastien, AU - Vignard,Julien, AU - Terrisse,Hélène, AU - Mirey,Gladys, AU - Audinot,Jean-Nicolas, AU - Theodorou,Vassilia, AU - Ropers,Marie-Hélène, AU - Robert,Hervé, AU - Mercier-Bonin,Muriel, Y1 - 2020/09/17/ PY - 2020/07/17/received PY - 2020/09/07/revised PY - 2020/09/08/accepted PY - 2020/12/1/entrez PY - 2020/12/2/pubmed PY - 2020/12/2/medline KW - Colloidal silver KW - Genotoxicity KW - Gut toxicity KW - High-resolution chemical imaging KW - Repeated exposure in vitro KW - Silver nanoparticles SP - 142324 EP - 142324 JF - The Science of the total environment JO - Sci Total Environ VL - 754 N2 - Colloidal silver products are sold for a wide range of disinfectant and health applications. This has increased the potential for human exposure to silver nanoparticles (AgNPs) and ions (Ag+), for which oral ingestion is considered to be a major route of exposure. Our objective was to evaluate and compare the toxicity of two commercially available colloidal silver products on two human intestinal epithelial models under realistic exposure conditions. Mesosilver™ and AgC were characterized and a concentration range between 0.1 and 12 μg/mL chosen. Caco-2 cells vs. co-culture of Caco-2 and mucus-secreting HT29-MTX cells (90/10) were used. Repeated exposure was carried out to determine cell viability over 18 days of cell differentiation in 24-well plates. Selected concentrations (0.1, 1, and 3 μg/mL) were tested on cells cultured in E-plates and Transwells with the same repeated exposure regimen, to determine cell impedance, and cell viability and trans-epithelial electrical resistance (TEER), respectively. Silver uptake, intracellular localisation, and translocation were determined by CytoViva™, HIM-SIMS, and ICP-MS. Genotoxicity was determined on acutely-exposed proliferating Caco-2 cells by γH2AX immunofluorescence staining. Repeated exposure of a given concentration of AgC, which is composed solely of ionic silver, generally exerted more toxic effects on Caco-2 cells than Mesosilver™, which contains a mix of AgNPs and ionic silver. Due to its patchy structure, the presence of mucus in the Caco-2/HT29-MTX co-culture only slightly mitigated the deleterious effects on cell viability. Increased genotoxicity was observed for AgC on proliferating Caco-2 cells. Silver uptake, intracellular localisation, and translocation were similar. In conclusion, Mesosilver™ and AgC colloidal silver products show different levels of gut toxicity due to the forms of distinct silver (AgNPs and/or Ag+) contained within. This study highlights the applicability of high-resolution (chemical) imaging to detect and localize silver and provides insights into its uptake mechanisms, intracellular fate and cellular effects. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/33254900/Repeated_exposure_of_Caco-2_versus_Caco-2/HT29-MTX_intestinal_cell_models_to_(nano)silver_in_vitro:_Comparison_of_two_commercially_available_colloidal_silver_products. L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)35853-8 DB - PRIME DP - Unbound Medicine ER -