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Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts.
Molecules. 2020 Nov 25; 25(23)M

Abstract

Breynia retusa (Dennst.) Alston (also known as Cup Saucer plant) is a food plant with wide applications in traditional medicine, particularly in Ayurveda. Extracts obtained with four solvents (dichloromethane, methanol, ethyl acetate and water), from three plant parts, (fruit, leaf and bark) were obtained. Extracts were tested for total phenolic, flavonoid content and antioxidant activities using a battery of assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating. Enzyme inhibitory effects were investigated using acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase as target enzymes. Results showed that the methanolic bark extract exhibited significant radical scavenging activity (DPPH: 202.09 ± 0.15; ABTS: 490.12 ± 0.18 mg Trolox equivalent (TE)/g), reducing potential (FRAP: 325.86 ± 4.36: CUPRAC: 661.82 ± 0.40 mg TE/g) and possessed the highest TAC (3.33 ± 0.13 mmol TE/g). The methanolic extracts were subjected to LC-DAD-MSn and NMR analysis. A two-column LC method was developed to separate constituents, allowing to identify and quantify forty-four and fifteen constituents in bark and fruits, respectively. Main compound in bark was epicatechin-3-O-sulphate and isolation of compound was performed to confirm its identity. Bark extract contained catechins, procyanidins, gallic acid derivatives and the sulfur containing spiroketal named breynins. Aerial parts mostly contained flavonoid glycosides. Considering the bioassays, the methanolic bark extract resulted a potent tyrosinase (152.79 ± 0.27 mg kojic acid equivalent/g), α-amylase (0.99 ± 0.01 mmol acarbose equivalent ACAE/g) and α-glucosidase (2.16 ± 0.01 mmol ACAE/g) inhibitor. In conclusion, methanol is able to extract the efficiently the phytoconstituents of B. retusa and the bark is the most valuable source of compounds.

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Authors+Show Affiliations

Dall'Acqua S
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
Sinan KI
Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey.
Ferrarese I
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
Sut S
DAFNAE, Department of Agronomy, Food, Natural Resources, Animals and Environment, Agripolis Campus, University of Padova, 35020 Legnaro, Italy.
Bene K
Laboratoire de Botanique et Phytothérapie, Unité de Formation et de Recherche Sciences de la Nature, 02 BP 801 Abidjan 02, Université Nangui Abrogoua, CI-YM. IV98 Abidjan, Ivory Cost.
Mahomoodally MF
Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, 80832 Réduit, Mauritius.
Bibi Sadeer N
Department of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, 80832 Réduit, Mauritius.
Ak G
Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey.
Zengin G
Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey.

MeSH

AntioxidantsChromatographyChromatography, LiquidData MiningEnzyme InhibitorsFruitMagnetic Resonance SpectroscopyMass SpectrometryMolecular StructurePhytochemicalsPlant BarkPlant ExtractsPlant LeavesTracheophyta

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33255853

Citation

Dall'Acqua, Stefano, et al. "Chromatographic Separation of Breynia Retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents From Bioactive Extracts." Molecules (Basel, Switzerland), vol. 25, no. 23, 2020.
Dall'Acqua S, Sinan KI, Ferrarese I, et al. Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts. Molecules. 2020;25(23).
Dall'Acqua, S., Sinan, K. I., Ferrarese, I., Sut, S., Bene, K., Mahomoodally, M. F., Bibi Sadeer, N., Ak, G., & Zengin, G. (2020). Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts. Molecules (Basel, Switzerland), 25(23). https://doi.org/10.3390/molecules25235537
Dall'Acqua S, et al. Chromatographic Separation of Breynia Retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents From Bioactive Extracts. Molecules. 2020 Nov 25;25(23) PubMed PMID: 33255853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts. AU - Dall'Acqua,Stefano, AU - Sinan,Kouadio Ibrahime, AU - Ferrarese,Irene, AU - Sut,Stefania, AU - Bene,Kouadio, AU - Mahomoodally,Mohamad Fawzi, AU - Bibi Sadeer,Nabeelah, AU - Ak,Gunes, AU - Zengin,Gokhan, Y1 - 2020/11/25/ PY - 2020/10/15/received PY - 2020/11/10/revised PY - 2020/11/17/accepted PY - 2020/12/1/entrez PY - 2020/12/2/pubmed PY - 2021/4/7/medline KW - Cup Saucer plant KW - antioxidant KW - bioactive compounds KW - enzyme inhibitors KW - traditional medicine JF - Molecules (Basel, Switzerland) JO - Molecules VL - 25 IS - 23 N2 - Breynia retusa (Dennst.) Alston (also known as Cup Saucer plant) is a food plant with wide applications in traditional medicine, particularly in Ayurveda. Extracts obtained with four solvents (dichloromethane, methanol, ethyl acetate and water), from three plant parts, (fruit, leaf and bark) were obtained. Extracts were tested for total phenolic, flavonoid content and antioxidant activities using a battery of assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating. Enzyme inhibitory effects were investigated using acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase as target enzymes. Results showed that the methanolic bark extract exhibited significant radical scavenging activity (DPPH: 202.09 ± 0.15; ABTS: 490.12 ± 0.18 mg Trolox equivalent (TE)/g), reducing potential (FRAP: 325.86 ± 4.36: CUPRAC: 661.82 ± 0.40 mg TE/g) and possessed the highest TAC (3.33 ± 0.13 mmol TE/g). The methanolic extracts were subjected to LC-DAD-MSn and NMR analysis. A two-column LC method was developed to separate constituents, allowing to identify and quantify forty-four and fifteen constituents in bark and fruits, respectively. Main compound in bark was epicatechin-3-O-sulphate and isolation of compound was performed to confirm its identity. Bark extract contained catechins, procyanidins, gallic acid derivatives and the sulfur containing spiroketal named breynins. Aerial parts mostly contained flavonoid glycosides. Considering the bioassays, the methanolic bark extract resulted a potent tyrosinase (152.79 ± 0.27 mg kojic acid equivalent/g), α-amylase (0.99 ± 0.01 mmol acarbose equivalent ACAE/g) and α-glucosidase (2.16 ± 0.01 mmol ACAE/g) inhibitor. In conclusion, methanol is able to extract the efficiently the phytoconstituents of B. retusa and the bark is the most valuable source of compounds. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/33255853/Chromatographic_Separation_of_Breynia_retusa__Dennst___Alston_Bark_Fruit_and_Leaf_Constituents_from_Bioactive_Extracts_ DB - PRIME DP - Unbound Medicine ER -