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[Effects of electroacupuncture on miR-34a-5p/SIRT1 signaling in the trigeminal ganglion of rats with migraine].
Zhen Ci Yan Jiu. 2020 Nov 25; 45(11):868-74.ZC

Abstract

OBJECTIVE

To investigate the effect of electroacupuncture(EA) on miR-34a-5p, silent mating type information regulation 2 homolog-1 (SIRT1) and nuclear factor-κB subunit p65 (NF-κB p65) in the trigeminal ganglion of rats with migraine, so as to explore the mechanisms of EA underlying prevention of migraine.

METHODS

Male SD rats were randomly divi-ded into normal, sham operation, model, EA, and EA plus EX527(a SIRT1 inhibitor) groups, with 10 rats in each group. The rat model of migraine was established by electrical stimulation of the trigeminal ganglion. Before modeling, EA was applied at "Waiguan"(TE5) and "Fengchi"(GB20) for 20 min each time, once a day for 5 consecutive days, and intraperitoneal injection of EX527 (5 mg/kg) every day simultaneously. Serum prostaglandin E2 (PGE2) and calcitonin gene-related peptide (CGRP) concentrations were measured by enzyme-linked immunosorbent assay. The levels of miR-34a-5p, SIRT1 and interleukin-1β (IL-1β) mRNA,and protein expression of SIRT1, IL-1β, NF-κB p65, NF-κB Ac-p65 and cyclooxygenase-2 (COX2) in trigeminal ganglia were detected by real-time quantitative PCR and Western blot, separately.

RESULTS

The serum concentrations of PGE2 and CGRP, the expression of miR-34a-5p, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression in the trigeminal ganglion were remarkably increased (P<0.05), while the SIRT1 mRNA and protein decreased (P<0.05) in the model group in contrast to the normal group. Following EA intervention, the serum PGE2 and CGRP concentrations, miR-34a-5p expression, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression were significantly down-regulated (P<0.05), and SIRT1 mRNA and protein significantly up-regulated (P<0.05). Compared with the EA group, the serum concentrations of PGE2 and CGRP, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expressions increased (P<0.05), and SIRT1 protein decreased (P<0.05) in the EA plus EX527 group.

CONCLUSION

In migraine rats, EA can inhibit miR-34a-5p expression in the trigeminal ganglion, increase SIRT1 expression, down-regulate IL-1β/COX2 inflammation signals, reduce PGE2 synthesis, and thus redue CGRP released from the peripheral terminals, which may be one of the mechanisms of EA in preventing migraine.

Authors+Show Affiliations

College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China; The Second Affiliated Hospital, Guilin Medical University, Guilin 541100, Guangxi Province.College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China; The Second Affiliated Hospital, Guilin Medical University, Guilin 541100, Guangxi Province.The Second Xiangya Hospital, Central South University, Changsha 410038.College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China.College of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China.Graduate School, Beijing University of Chinese Medicine, Beijing 100078.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33269829

Citation

Zhang, Hui, et al. "[Effects of Electroacupuncture On miR-34a-5p/SIRT1 Signaling in the Trigeminal Ganglion of Rats With Migraine]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 45, no. 11, 2020, pp. 868-74.
Zhang H, He SD, Zong DD, et al. [Effects of electroacupuncture on miR-34a-5p/SIRT1 signaling in the trigeminal ganglion of rats with migraine]. Zhen Ci Yan Jiu. 2020;45(11):868-74.
Zhang, H., He, S. D., Zong, D. D., Zhang, X. M., Luo, J., & Zheng, J. K. (2020). [Effects of electroacupuncture on miR-34a-5p/SIRT1 signaling in the trigeminal ganglion of rats with migraine]. Zhen Ci Yan Jiu = Acupuncture Research, 45(11), 868-74. https://doi.org/10.13702/j.1000-0607.200378
Zhang H, et al. [Effects of Electroacupuncture On miR-34a-5p/SIRT1 Signaling in the Trigeminal Ganglion of Rats With Migraine]. Zhen Ci Yan Jiu. 2020 Nov 25;45(11):868-74. PubMed PMID: 33269829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on miR-34a-5p/SIRT1 signaling in the trigeminal ganglion of rats with migraine]. AU - Zhang,Hui, AU - He,Sheng-Dong, AU - Zong,Dan-Dan, AU - Zhang,Xue-Mei, AU - Luo,Jing, AU - Zheng,Jia-Kun, PY - 2020/12/3/entrez PY - 2020/12/4/pubmed PY - 2020/12/22/medline KW - Electroacupuncture KW - Migraine KW - NF-κB p65 KW - SIRT1 KW - Trigeminal ganglion KW - miR-34a-5p SP - 868 EP - 74 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 45 IS - 11 N2 - OBJECTIVE: To investigate the effect of electroacupuncture(EA) on miR-34a-5p, silent mating type information regulation 2 homolog-1 (SIRT1) and nuclear factor-κB subunit p65 (NF-κB p65) in the trigeminal ganglion of rats with migraine, so as to explore the mechanisms of EA underlying prevention of migraine. METHODS: Male SD rats were randomly divi-ded into normal, sham operation, model, EA, and EA plus EX527(a SIRT1 inhibitor) groups, with 10 rats in each group. The rat model of migraine was established by electrical stimulation of the trigeminal ganglion. Before modeling, EA was applied at "Waiguan"(TE5) and "Fengchi"(GB20) for 20 min each time, once a day for 5 consecutive days, and intraperitoneal injection of EX527 (5 mg/kg) every day simultaneously. Serum prostaglandin E2 (PGE2) and calcitonin gene-related peptide (CGRP) concentrations were measured by enzyme-linked immunosorbent assay. The levels of miR-34a-5p, SIRT1 and interleukin-1β (IL-1β) mRNA,and protein expression of SIRT1, IL-1β, NF-κB p65, NF-κB Ac-p65 and cyclooxygenase-2 (COX2) in trigeminal ganglia were detected by real-time quantitative PCR and Western blot, separately. RESULTS: The serum concentrations of PGE2 and CGRP, the expression of miR-34a-5p, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression in the trigeminal ganglion were remarkably increased (P<0.05), while the SIRT1 mRNA and protein decreased (P<0.05) in the model group in contrast to the normal group. Following EA intervention, the serum PGE2 and CGRP concentrations, miR-34a-5p expression, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expression were significantly down-regulated (P<0.05), and SIRT1 mRNA and protein significantly up-regulated (P<0.05). Compared with the EA group, the serum concentrations of PGE2 and CGRP, IL-1β mRNA and protein, NF-κB p65, NF-κB Ac-p65 and COX2 protein expressions increased (P<0.05), and SIRT1 protein decreased (P<0.05) in the EA plus EX527 group. CONCLUSION: In migraine rats, EA can inhibit miR-34a-5p expression in the trigeminal ganglion, increase SIRT1 expression, down-regulate IL-1β/COX2 inflammation signals, reduce PGE2 synthesis, and thus redue CGRP released from the peripheral terminals, which may be one of the mechanisms of EA in preventing migraine. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/33269829/[Effects_of_electroacupuncture_on_miR_34a_5p/SIRT1_signaling_in_the_trigeminal_ganglion_of_rats_with_migraine]_ L2 - https://medlineplus.gov/migraine.html DB - PRIME DP - Unbound Medicine ER -