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Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods.
PLoS One. 2020; 15(12):e0243175.Plos

Abstract

A high-performance liquid chromatography (HPLC) method with relative molar sensitivity (RMS) based on 1H quantitative NMR spectroscopy (1H-qNMR) has been developed for food ingredients such as acteoside (verbascoside) and pedaliin (pedalitin-6-O-glucoside) without requiring authentic and identical standards as the reliable analytical methods. This method is used methyl 4-hydroxybenzoate (MHB) as an alternative reference standard. Each RMS is also calculated from the ratio of each analyte's molar absorption coefficient to that of MHB after correcting the purities of the analytes and reference standard by 1H-qNMR. Therefore, this method can quantify several analytes with metrological traceability to the International System of Units (SI) using the RMS and one alternative reference standard. In this study, the content of acteoside and pedaliin in several samples, such as dried sesame leaf powders and commercially processed foods, can be determined by the proposed RMS method and demonstrated in good agreement that obtained by a conventional method. Moreover, the proposed method yields analytical data with SI-traceability without the need for an authentic and identical analyte standard. Thus, the proposed RMS method is a useful and practical tool for determining acteoside and pedaliin in terms of the accuracy of quantitative values, the routine analysis, and the cost of reagents.

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Authors+Show Affiliations

Ohtsuki T
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Matsuoka K
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Fuji Y
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Nishizaki Y
Division of Food Additives, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Masumoto N
Division of Food Additives, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Sugimoto N
Division of Food Additives, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Sato K
Division of Food Additives, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Matsufuji H
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.

MeSH

Chromatography, High Pressure LiquidFlavonesFood AnalysisGlucosidesPhenolsPlant LeavesProton Magnetic Resonance SpectroscopySesamum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33270771

Citation

Ohtsuki, Takashi, et al. "Development of an HPLC Method With Relative Molar Sensitivity Based On 1H-qNMR to Determine Acteoside and Pedaliin in Dried Sesame Leaf Powders and Processed Foods." PloS One, vol. 15, no. 12, 2020, pp. e0243175.
Ohtsuki T, Matsuoka K, Fuji Y, et al. Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods. PLoS One. 2020;15(12):e0243175.
Ohtsuki, T., Matsuoka, K., Fuji, Y., Nishizaki, Y., Masumoto, N., Sugimoto, N., Sato, K., & Matsufuji, H. (2020). Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods. PloS One, 15(12), e0243175. https://doi.org/10.1371/journal.pone.0243175
Ohtsuki T, et al. Development of an HPLC Method With Relative Molar Sensitivity Based On 1H-qNMR to Determine Acteoside and Pedaliin in Dried Sesame Leaf Powders and Processed Foods. PLoS One. 2020;15(12):e0243175. PubMed PMID: 33270771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods. AU - Ohtsuki,Takashi, AU - Matsuoka,Kiyoaki, AU - Fuji,Yushiro, AU - Nishizaki,Yuzo, AU - Masumoto,Naoko, AU - Sugimoto,Naoki, AU - Sato,Kyoko, AU - Matsufuji,Hiroshi, Y1 - 2020/12/03/ PY - 2020/08/18/received PY - 2020/11/17/accepted PY - 2020/12/3/entrez PY - 2020/12/4/pubmed PY - 2021/1/20/medline SP - e0243175 EP - e0243175 JF - PloS one JO - PLoS One VL - 15 IS - 12 N2 - A high-performance liquid chromatography (HPLC) method with relative molar sensitivity (RMS) based on 1H quantitative NMR spectroscopy (1H-qNMR) has been developed for food ingredients such as acteoside (verbascoside) and pedaliin (pedalitin-6-O-glucoside) without requiring authentic and identical standards as the reliable analytical methods. This method is used methyl 4-hydroxybenzoate (MHB) as an alternative reference standard. Each RMS is also calculated from the ratio of each analyte's molar absorption coefficient to that of MHB after correcting the purities of the analytes and reference standard by 1H-qNMR. Therefore, this method can quantify several analytes with metrological traceability to the International System of Units (SI) using the RMS and one alternative reference standard. In this study, the content of acteoside and pedaliin in several samples, such as dried sesame leaf powders and commercially processed foods, can be determined by the proposed RMS method and demonstrated in good agreement that obtained by a conventional method. Moreover, the proposed method yields analytical data with SI-traceability without the need for an authentic and identical analyte standard. Thus, the proposed RMS method is a useful and practical tool for determining acteoside and pedaliin in terms of the accuracy of quantitative values, the routine analysis, and the cost of reagents. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/33270771/Development_of_an_HPLC_method_with_relative_molar_sensitivity_based_on_1H_qNMR_to_determine_acteoside_and_pedaliin_in_dried_sesame_leaf_powders_and_processed_foods_ DB - PRIME DP - Unbound Medicine ER -