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Urinary clusterin and cystatin B as biomarkers of tubular injury in dogs following envenomation by the European adder.
Res Vet Sci. 2021 Jan; 134:12-18.RV

Abstract

Diagnosing acute kidney injury remains a challenge since the established renal biomarkers, serum creatinine (sCr) and symmetric dimethylarginine (SDMA) reflect glomerular function and not tubular injury. Sensitive tubular markers such as urinary clusterin (uClust) and cystatin B (uCysB) have been proposed to detect AKI at an earlier stage. Since envenomation by the European adder (Vipera berus berus) could serve as a spontaneous disease model of AKI we investigated these new biomarkers in affected dogs. Concentrations of uClust and uCysB as well as sCr and SDMA were analyzed retrospectively in stored samples from 26 dogs with snake envenomation and 13 healthy controls. Higher concentrations of uClust (P < 0.012) and uCysB (P < 0.001) were observed in the snake-envenomed group. Normalization of uClust and uCysB to urinary creatinine did not alter the results. No differences were observed in sCr and SDMA between the snake-envenomed group and the healthy control group. Spearman rank correlation analysis revealed a strong association of uClust with uCysB in the snake-envenomed dogs (r = 0.75 P < 0.001) but not in the healthy controls. The high percentage of snake-envenomed dogs with increased uClust and uCysB concentrations in the absence of increased sCr and SDMA suggests renal tubular injury in the affected dogs. Larger prospective case-controlled studies are warranted to evaluate the clinical utility and prognostic value of these biomarkers.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Faculty of Veterinary Medicine, Department of Equine and Small Animal Medicine, Internal Medicine Section, University of Helsinki, P.O. Box 57, 00014 University of Helsinki, Finland. Electronic address: emilia.gordin@helsinki.fi.

Abdominal Center Nephrology, Helsinki University Hospital, Folkhälsan Research Center, University of Helsinki, Helsinki, Finland.

Faculty of Veterinary Medicine, Department of Equine and Small Animal Medicine, Internal Medicine Section, University of Helsinki, P.O. Box 57, 00014 University of Helsinki, Finland.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, United States.

Faculty of Veterinary Medicine, Department of Equine and Small Animal Medicine, Internal Medicine Section, University of Helsinki, P.O. Box 57, 00014 University of Helsinki, Finland.

MeSH

Acute Kidney InjuryAnimalsArginineBiomarkersCase-Control StudiesClusterinCohort StudiesCreatinineCystatin BDog DiseasesDogsFemaleKidney Function TestsMaleProspective StudiesRetrospective StudiesSnake BitesViperidae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33278755

Citation

Gordin, E, et al. "Urinary Clusterin and Cystatin B as Biomarkers of Tubular Injury in Dogs Following Envenomation By the European Adder." Research in Veterinary Science, vol. 134, 2021, pp. 12-18.

Gordin E, Gordin D, Viitanen S, et al. Urinary clusterin and cystatin B as biomarkers of tubular injury in dogs following envenomation by the European adder. Res Vet Sci. 2021;134:12-18.

Gordin, E., Gordin, D., Viitanen, S., Szlosek, D., Coyne, M., Farace, G., Murphy, R., Quinn, J., Yerramilli, M., Yerramilli, M., & Spillmann, T. (2021). Urinary clusterin and cystatin B as biomarkers of tubular injury in dogs following envenomation by the European adder. Research in Veterinary Science, 134, 12-18. https://doi.org/10.1016/j.rvsc.2020.11.019

Gordin E, et al. Urinary Clusterin and Cystatin B as Biomarkers of Tubular Injury in Dogs Following Envenomation By the European Adder. Res Vet Sci. 2021;134:12-18. PubMed PMID: 33278755.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Urinary clusterin and cystatin B as biomarkers of tubular injury in dogs following envenomation by the European adder. AU - Gordin,E, AU - Gordin,D, AU - Viitanen,S, AU - Szlosek,D, AU - Coyne,M, AU - Farace,G, AU - Murphy,R, AU - Quinn,J, AU - Yerramilli,Ma, AU - Yerramilli,Mu, AU - Spillmann,T, Y1 - 2020/11/24/ PY - 2020/06/09/received PY - 2020/10/14/revised PY - 2020/11/22/accepted PY - 2020/12/6/pubmed PY - 2021/3/23/medline PY - 2020/12/5/entrez KW - Acute kidney injury KW - Canine KW - Envenomation KW - European adder KW - Tubular biomarker SP - 12 EP - 18 JF - Research in veterinary science JO - Res Vet Sci VL - 134 N2 - Diagnosing acute kidney injury remains a challenge since the established renal biomarkers, serum creatinine (sCr) and symmetric dimethylarginine (SDMA) reflect glomerular function and not tubular injury. Sensitive tubular markers such as urinary clusterin (uClust) and cystatin B (uCysB) have been proposed to detect AKI at an earlier stage. Since envenomation by the European adder (Vipera berus berus) could serve as a spontaneous disease model of AKI we investigated these new biomarkers in affected dogs. Concentrations of uClust and uCysB as well as sCr and SDMA were analyzed retrospectively in stored samples from 26 dogs with snake envenomation and 13 healthy controls. Higher concentrations of uClust (P < 0.012) and uCysB (P < 0.001) were observed in the snake-envenomed group. Normalization of uClust and uCysB to urinary creatinine did not alter the results. No differences were observed in sCr and SDMA between the snake-envenomed group and the healthy control group. Spearman rank correlation analysis revealed a strong association of uClust with uCysB in the snake-envenomed dogs (r = 0.75 P < 0.001) but not in the healthy controls. The high percentage of snake-envenomed dogs with increased uClust and uCysB concentrations in the absence of increased sCr and SDMA suggests renal tubular injury in the affected dogs. Larger prospective case-controlled studies are warranted to evaluate the clinical utility and prognostic value of these biomarkers. SN - 1532-2661 UR - https://www.unboundmedicine.com/medline/citation/33278755/Urinary_clusterin_and_cystatin_B_as_biomarkers_of_tubular_injury_in_dogs_following_envenomation_by_the_European_adder_ DB - PRIME DP - Unbound Medicine ER -