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Differential plasma exosomal long non-coding RNAs expression profiles and their emerging role in E-cigarette users, cigarette, waterpipe, and dual smokers.
PLoS One. 2020; 15(12):e0243065.Plos

Abstract

Long non-coding RNAs (lncRNAs) are the varied set of transcripts that play a critical role in biological processes like gene regulation, transcription, post-transcriptional modification, and chromatin remodeling. Recent studies have reported the presence of lncRNAs in the exosomes that are involved in regulating cell-to-cell communication in lung pathologies including lung cancer, chronic obstructive pulmonary disease (COPD), asthma, and idiopathic pulmonary fibrosis (IPF). In this study, we compared the lncRNA profiles in the plasma-derived exosomes amongst non-smokers (NS), cigarette smokers (CS), E-cig users (E-cig), waterpipe smokers (WP) and dual smokers (CSWP) using GeneChip™ WT Pico kit for transcriptional profiling. We found alterations in a distinct set of lncRNAs among subjects exposed to E-cig vapor, cigarette smoke, waterpipe smoke and dual smoke with some overlaps. Gene enrichment analyses of the differentially expressed lncRNAs demonstrated enrichment in the lncRNAs involved in crucial biological processes including steroid metabolism, cell differentiation and proliferation. Thus, the characterized lncRNA profiles of the plasma-derived exosomes from smokers, vapers, waterpipe users, and dual smokers will help identify the biomarkers relevant to chronic lung diseases such as COPD, asthma or IPF.

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Authors+Show Affiliations

Kaur G
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, United States of America.
Singh K
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, United States of America.
Maremanda KP
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, United States of America.
Li D
Department of Clinical & Translational Research, University of Rochester Medical Center, Rochester, NY, United States of America.
Chand HS
Department of Immunology and Nanomedicine, Florida International University, Miami, FL, United States of America.
Rahman I
Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY, United States of America.

MeSH

Case-Control StudiesExosomesFemaleGene Expression ProfilingGene Expression RegulationGene Regulatory NetworksHumansMaleOligonucleotide Array Sequence AnalysisRNA, Long NoncodingTobacco SmokingVapingWater Pipe Smoking

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

33290406

Citation

Kaur, Gagandeep, et al. "Differential Plasma Exosomal Long Non-coding RNAs Expression Profiles and Their Emerging Role in E-cigarette Users, Cigarette, Waterpipe, and Dual Smokers." PloS One, vol. 15, no. 12, 2020, pp. e0243065.
Kaur G, Singh K, Maremanda KP, et al. Differential plasma exosomal long non-coding RNAs expression profiles and their emerging role in E-cigarette users, cigarette, waterpipe, and dual smokers. PLoS One. 2020;15(12):e0243065.
Kaur, G., Singh, K., Maremanda, K. P., Li, D., Chand, H. S., & Rahman, I. (2020). Differential plasma exosomal long non-coding RNAs expression profiles and their emerging role in E-cigarette users, cigarette, waterpipe, and dual smokers. PloS One, 15(12), e0243065. https://doi.org/10.1371/journal.pone.0243065
Kaur G, et al. Differential Plasma Exosomal Long Non-coding RNAs Expression Profiles and Their Emerging Role in E-cigarette Users, Cigarette, Waterpipe, and Dual Smokers. PLoS One. 2020;15(12):e0243065. PubMed PMID: 33290406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential plasma exosomal long non-coding RNAs expression profiles and their emerging role in E-cigarette users, cigarette, waterpipe, and dual smokers. AU - Kaur,Gagandeep, AU - Singh,Kameshwar, AU - Maremanda,Krishna P, AU - Li,Dongmei, AU - Chand,Hitendra S, AU - Rahman,Irfan, Y1 - 2020/12/08/ PY - 2020/09/14/received PY - 2020/11/15/accepted PY - 2020/12/8/entrez PY - 2020/12/9/pubmed PY - 2021/1/16/medline SP - e0243065 EP - e0243065 JF - PloS one JO - PLoS One VL - 15 IS - 12 N2 - Long non-coding RNAs (lncRNAs) are the varied set of transcripts that play a critical role in biological processes like gene regulation, transcription, post-transcriptional modification, and chromatin remodeling. Recent studies have reported the presence of lncRNAs in the exosomes that are involved in regulating cell-to-cell communication in lung pathologies including lung cancer, chronic obstructive pulmonary disease (COPD), asthma, and idiopathic pulmonary fibrosis (IPF). In this study, we compared the lncRNA profiles in the plasma-derived exosomes amongst non-smokers (NS), cigarette smokers (CS), E-cig users (E-cig), waterpipe smokers (WP) and dual smokers (CSWP) using GeneChip™ WT Pico kit for transcriptional profiling. We found alterations in a distinct set of lncRNAs among subjects exposed to E-cig vapor, cigarette smoke, waterpipe smoke and dual smoke with some overlaps. Gene enrichment analyses of the differentially expressed lncRNAs demonstrated enrichment in the lncRNAs involved in crucial biological processes including steroid metabolism, cell differentiation and proliferation. Thus, the characterized lncRNA profiles of the plasma-derived exosomes from smokers, vapers, waterpipe users, and dual smokers will help identify the biomarkers relevant to chronic lung diseases such as COPD, asthma or IPF. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/33290406/Differential_plasma_exosomal_long_non_coding_RNAs_expression_profiles_and_their_emerging_role_in_E_cigarette_users_cigarette_waterpipe_and_dual_smokers_ DB - PRIME DP - Unbound Medicine ER -