Tags

Type your tag names separated by a space and hit enter

SARS-CoV-2 Epitopes Are Recognized by a Public and Diverse Repertoire of Human T Cell Receptors.
Immunity. 2020 12 15; 53(6):1245-1257.e5.I

Abstract

Understanding the hallmarks of the immune response to SARS-CoV-2 is critical for fighting the COVID-19 pandemic. We assessed antibody and T cell reactivity in convalescent COVID-19 patients and healthy donors sampled both prior to and during the pandemic. Healthy donors examined during the pandemic exhibited increased numbers of SARS-CoV-2-specific T cells, but no humoral response. Their probable exposure to the virus resulted in either asymptomatic infection without antibody secretion or activation of preexisting immunity. In convalescent patients, we observed a public and diverse T cell response to SARS-CoV-2 epitopes, revealing T cell receptor (TCR) motifs with germline-encoded features. Bulk CD4+ and CD8+ T cell responses to the spike protein were mediated by groups of homologous TCRs, some of them shared across multiple donors. Overall, our results demonstrate that the T cell response to SARS-CoV-2, including the identified set of TCRs, can serve as a useful biomarker for surveying antiviral immunity.

Authors+Show Affiliations

National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.Eindhoven University of Technology, Eindhoven, the Netherlands.Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia.Pirogov Russian Medical State University, Moscow, Russia; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.National Research Center for Hematology, Moscow, Russia.Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia; Pirogov Russian Medical State University, Moscow, Russia; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. Electronic address: mikhail.shugay@gmail.com.National Research Center for Hematology, Moscow, Russia. Electronic address: efimov.g@blood.ru.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33326767

Citation

Shomuradova, Alina S., et al. "SARS-CoV-2 Epitopes Are Recognized By a Public and Diverse Repertoire of Human T Cell Receptors." Immunity, vol. 53, no. 6, 2020, pp. 1245-1257.e5.
Shomuradova AS, Vagida MS, Sheetikov SA, et al. SARS-CoV-2 Epitopes Are Recognized by a Public and Diverse Repertoire of Human T Cell Receptors. Immunity. 2020;53(6):1245-1257.e5.
Shomuradova, A. S., Vagida, M. S., Sheetikov, S. A., Zornikova, K. V., Kiryukhin, D., Titov, A., Peshkova, I. O., Khmelevskaya, A., Dianov, D. V., Malasheva, M., Shmelev, A., Serdyuk, Y., Bagaev, D. V., Pivnyuk, A., Shcherbinin, D. S., Maleeva, A. V., Shakirova, N. T., Pilunov, A., Malko, D. B., ... Efimov, G. A. (2020). SARS-CoV-2 Epitopes Are Recognized by a Public and Diverse Repertoire of Human T Cell Receptors. Immunity, 53(6), 1245-e5. https://doi.org/10.1016/j.immuni.2020.11.004
Shomuradova AS, et al. SARS-CoV-2 Epitopes Are Recognized By a Public and Diverse Repertoire of Human T Cell Receptors. Immunity. 2020 12 15;53(6):1245-1257.e5. PubMed PMID: 33326767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2 Epitopes Are Recognized by a Public and Diverse Repertoire of Human T Cell Receptors. AU - Shomuradova,Alina S, AU - Vagida,Murad S, AU - Sheetikov,Savely A, AU - Zornikova,Ksenia V, AU - Kiryukhin,Dmitry, AU - Titov,Aleksei, AU - Peshkova,Iuliia O, AU - Khmelevskaya,Alexandra, AU - Dianov,Dmitry V, AU - Malasheva,Maria, AU - Shmelev,Anton, AU - Serdyuk,Yana, AU - Bagaev,Dmitry V, AU - Pivnyuk,Anastasia, AU - Shcherbinin,Dmitrii S, AU - Maleeva,Alexandra V, AU - Shakirova,Naina T, AU - Pilunov,Artem, AU - Malko,Dmitry B, AU - Khamaganova,Ekaterina G, AU - Biderman,Bella, AU - Ivanov,Alexander, AU - Shugay,Mikhail, AU - Efimov,Grigory A, Y1 - 2020/11/13/ PY - 2020/06/13/received PY - 2020/09/02/revised PY - 2020/11/09/accepted PY - 2020/12/16/entrez PY - 2020/12/17/pubmed PY - 2020/12/17/medline KW - COVID-19 KW - HLA-A∗02:01 KW - MHC KW - SARS-CoV-2 KW - Spike KW - T cell epitopes KW - T cell receptor KW - T cell response KW - TCR sequencing KW - clonal structure SP - 1245 EP - 1257.e5 JF - Immunity JO - Immunity VL - 53 IS - 6 N2 - Understanding the hallmarks of the immune response to SARS-CoV-2 is critical for fighting the COVID-19 pandemic. We assessed antibody and T cell reactivity in convalescent COVID-19 patients and healthy donors sampled both prior to and during the pandemic. Healthy donors examined during the pandemic exhibited increased numbers of SARS-CoV-2-specific T cells, but no humoral response. Their probable exposure to the virus resulted in either asymptomatic infection without antibody secretion or activation of preexisting immunity. In convalescent patients, we observed a public and diverse T cell response to SARS-CoV-2 epitopes, revealing T cell receptor (TCR) motifs with germline-encoded features. Bulk CD4+ and CD8+ T cell responses to the spike protein were mediated by groups of homologous TCRs, some of them shared across multiple donors. Overall, our results demonstrate that the T cell response to SARS-CoV-2, including the identified set of TCRs, can serve as a useful biomarker for surveying antiviral immunity. SN - 1097-4180 UR - https://www.unboundmedicine.com/medline/citation/33326767/SARS_CoV_2_Epitopes_Are_Recognized_by_a_Public_and_Diverse_Repertoire_of_Human_T_Cell_Receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1074-7613(20)30469-6 DB - PRIME DP - Unbound Medicine ER -