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Tryptophan accumulation in Saccharomyces cerevisiae under the influence of an artificial yeast TRP gene cluster.
Yeast. 1987 Jun; 3(2):95-105.Y

Abstract

Plasmid pME559, carrying all five yeast TRP genes, was constructed. This plasmid is a yeast/Escherichia coli shuttle vector based on pBR322 and 2 micron-DNA sequences derived from plasmid pJDB207. We studied in yeast (i) the stability of the plasmid under selective and non-selective conditions, (ii) expression of all five TRP genes and (iii) tryptophan accumulation in yeast transformants. These studies were conducted in comparison with an earlier construction, pME554, which differs from plasmid pME559 in the expression of the TRP1 gene and which carries the TRP2 wild type instead of the TRP2fbr mutant allele. For stable maintenance of the plasmids in yeast a selection was necessary. Plasmid pME559 displayed normal expression of all TRP genes, and enzyme levels on average 23-fold higher than in the wild type strain were found. In comparison, the maximal tryptophan flux observed in such a plasmid-carrying strain was about ten-fold higher than the maximal flux capacity in the wild type strain.

Authors+Show Affiliations

Prasad R
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Niederberger P
No affiliation info available
Hütter R
No affiliation info available

MeSH

AllelesAnthranilate SynthaseEscherichia coliGene Expression RegulationGenes, FungalGenetic VectorsMultigene FamilyMutationPlasmidsSaccharomyces cerevisiaeTransformation, GeneticTryptophan

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3332969

Citation

Prasad, R, et al. "Tryptophan Accumulation in Saccharomyces Cerevisiae Under the Influence of an Artificial Yeast TRP Gene Cluster." Yeast (Chichester, England), vol. 3, no. 2, 1987, pp. 95-105.
Prasad R, Niederberger P, Hütter R. Tryptophan accumulation in Saccharomyces cerevisiae under the influence of an artificial yeast TRP gene cluster. Yeast. 1987;3(2):95-105.
Prasad, R., Niederberger, P., & Hütter, R. (1987). Tryptophan accumulation in Saccharomyces cerevisiae under the influence of an artificial yeast TRP gene cluster. Yeast (Chichester, England), 3(2), 95-105.
Prasad R, Niederberger P, Hütter R. Tryptophan Accumulation in Saccharomyces Cerevisiae Under the Influence of an Artificial Yeast TRP Gene Cluster. Yeast. 1987;3(2):95-105. PubMed PMID: 3332969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tryptophan accumulation in Saccharomyces cerevisiae under the influence of an artificial yeast TRP gene cluster. AU - Prasad,R, AU - Niederberger,P, AU - Hütter,R, PY - 1987/6/1/pubmed PY - 1987/6/1/medline PY - 1987/6/1/entrez SP - 95 EP - 105 JF - Yeast (Chichester, England) JO - Yeast VL - 3 IS - 2 N2 - Plasmid pME559, carrying all five yeast TRP genes, was constructed. This plasmid is a yeast/Escherichia coli shuttle vector based on pBR322 and 2 micron-DNA sequences derived from plasmid pJDB207. We studied in yeast (i) the stability of the plasmid under selective and non-selective conditions, (ii) expression of all five TRP genes and (iii) tryptophan accumulation in yeast transformants. These studies were conducted in comparison with an earlier construction, pME554, which differs from plasmid pME559 in the expression of the TRP1 gene and which carries the TRP2 wild type instead of the TRP2fbr mutant allele. For stable maintenance of the plasmids in yeast a selection was necessary. Plasmid pME559 displayed normal expression of all TRP genes, and enzyme levels on average 23-fold higher than in the wild type strain were found. In comparison, the maximal tryptophan flux observed in such a plasmid-carrying strain was about ten-fold higher than the maximal flux capacity in the wild type strain. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/3332969/Tryptophan_accumulation_in_Saccharomyces_cerevisiae_under_the_influence_of_an_artificial_yeast_TRP_gene_cluster_ DB - PRIME DP - Unbound Medicine ER -