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Regulation of the enzymes at the branchpoint between the citric acid cycle and the glyoxylate bypass in Escherichia coli.
Biochem Soc Symp. 1987; 54:93-101.BS

Abstract

During growth of Escherichia coli on acetate, the glyoxylate bypass supplies the precursors needed for biosynthesis. The glyoxylate bypass enzyme isocitrate lyase competes with the citric acid cycle enzyme isocitrate dehydrogenase for the available isocitrate. We have studied the control of metabolic flux at this branchpoint by examining the regulatory properties of the enzymes concerned. Isocitrate dehydrogenase is controlled by reversible phosphorylation catalysed by a bifunctional kinase/phosphatase whose activities are regulated by isocitrate, biosynthetic precursors and adenine nucleotides. The flux through isocitrate lyase is mainly controlled by the intracellular concentration of isocitrate. The phosphorylation system responds to the availability of energy and precursors and maintains isocitrate at a concentration high enough to sustain the flux through the glyoxylate bypass necessary for biosynthesis.

Authors+Show Affiliations

Department of Biochemistry, University of Glasgow, U.K.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

3333001

Citation

Nimmo, H G., et al. "Regulation of the Enzymes at the Branchpoint Between the Citric Acid Cycle and the Glyoxylate Bypass in Escherichia Coli." Biochemical Society Symposium, vol. 54, 1987, pp. 93-101.
Nimmo HG, Borthwick AC, el-Mansi EM, et al. Regulation of the enzymes at the branchpoint between the citric acid cycle and the glyoxylate bypass in Escherichia coli. Biochem Soc Symp. 1987;54:93-101.
Nimmo, H. G., Borthwick, A. C., el-Mansi, E. M., Holms, W. H., MacKintosh, C., & Nimmo, G. A. (1987). Regulation of the enzymes at the branchpoint between the citric acid cycle and the glyoxylate bypass in Escherichia coli. Biochemical Society Symposium, 54, 93-101.
Nimmo HG, et al. Regulation of the Enzymes at the Branchpoint Between the Citric Acid Cycle and the Glyoxylate Bypass in Escherichia Coli. Biochem Soc Symp. 1987;54:93-101. PubMed PMID: 3333001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the enzymes at the branchpoint between the citric acid cycle and the glyoxylate bypass in Escherichia coli. AU - Nimmo,H G, AU - Borthwick,A C, AU - el-Mansi,E M, AU - Holms,W H, AU - MacKintosh,C, AU - Nimmo,G A, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 93 EP - 101 JF - Biochemical Society symposium JO - Biochem Soc Symp VL - 54 N2 - During growth of Escherichia coli on acetate, the glyoxylate bypass supplies the precursors needed for biosynthesis. The glyoxylate bypass enzyme isocitrate lyase competes with the citric acid cycle enzyme isocitrate dehydrogenase for the available isocitrate. We have studied the control of metabolic flux at this branchpoint by examining the regulatory properties of the enzymes concerned. Isocitrate dehydrogenase is controlled by reversible phosphorylation catalysed by a bifunctional kinase/phosphatase whose activities are regulated by isocitrate, biosynthetic precursors and adenine nucleotides. The flux through isocitrate lyase is mainly controlled by the intracellular concentration of isocitrate. The phosphorylation system responds to the availability of energy and precursors and maintains isocitrate at a concentration high enough to sustain the flux through the glyoxylate bypass necessary for biosynthesis. SN - 0067-8694 UR - https://www.unboundmedicine.com/medline/citation/3333001/Regulation_of_the_enzymes_at_the_branchpoint_between_the_citric_acid_cycle_and_the_glyoxylate_bypass_in_Escherichia_coli_ DB - PRIME DP - Unbound Medicine ER -