Tags

Type your tag names separated by a space and hit enter

High affinity phlorizin binding to the LLC-PK1 cells exhibits a sodium:phlorizin stoichiometry of 2:1.
J Biol Chem 1988; 263(1):187-92JB

Abstract

The phlorizin binding properties of luminal membrane vesicles isolated from the LLC-PK1 cells, a continuous epithelial cell line derived from pig kidney, are studied. Scatchard analysis of this binding indicates the existence of a single high affinity sodium-dependent site with KD = 0.4 microM at 266 mM sodium. The specificity properties of this site indicate that it represents the binding of phlorizin to the hexose binding site of the sodium-dependent D-glucose transporter previously identified in this cell line. Both phlorizin equilibrium binding and the rate of phlorizin binding were found to be sigmoidal functions of sodium concentration. A Hill analysis of these data was consistent with a sodium:phlorizin stoichiometry of 2:1 in good agreement with the sodium:glucose stoichiometry already established in these cells. Phlorizin dissociation was also found to be sodium-dependent. On the basis of the phlorizin binding data presented here, a number of models of the binding of phlorizin and sodium to the transporter can be excluded. An analysis of a random binding model consistent with the data is presented. The significance of the LLC-PK1 sodium-dependent D-glucose transporter as a model system for related renal and intestinal transporters is discussed.

Authors+Show Affiliations

Department of Physiology, Armed Forces Radiobiological Research Institute, Bethesda, Maryland 20892.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3335496

Citation

Moran, A, et al. "High Affinity Phlorizin Binding to the LLC-PK1 Cells Exhibits a Sodium:phlorizin Stoichiometry of 2:1." The Journal of Biological Chemistry, vol. 263, no. 1, 1988, pp. 187-92.
Moran A, Davis LJ, Turner RJ. High affinity phlorizin binding to the LLC-PK1 cells exhibits a sodium:phlorizin stoichiometry of 2:1. J Biol Chem. 1988;263(1):187-92.
Moran, A., Davis, L. J., & Turner, R. J. (1988). High affinity phlorizin binding to the LLC-PK1 cells exhibits a sodium:phlorizin stoichiometry of 2:1. The Journal of Biological Chemistry, 263(1), pp. 187-92.
Moran A, Davis LJ, Turner RJ. High Affinity Phlorizin Binding to the LLC-PK1 Cells Exhibits a Sodium:phlorizin Stoichiometry of 2:1. J Biol Chem. 1988 Jan 5;263(1):187-92. PubMed PMID: 3335496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High affinity phlorizin binding to the LLC-PK1 cells exhibits a sodium:phlorizin stoichiometry of 2:1. AU - Moran,A, AU - Davis,L J, AU - Turner,R J, PY - 1988/1/5/pubmed PY - 1988/1/5/medline PY - 1988/1/5/entrez SP - 187 EP - 92 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 263 IS - 1 N2 - The phlorizin binding properties of luminal membrane vesicles isolated from the LLC-PK1 cells, a continuous epithelial cell line derived from pig kidney, are studied. Scatchard analysis of this binding indicates the existence of a single high affinity sodium-dependent site with KD = 0.4 microM at 266 mM sodium. The specificity properties of this site indicate that it represents the binding of phlorizin to the hexose binding site of the sodium-dependent D-glucose transporter previously identified in this cell line. Both phlorizin equilibrium binding and the rate of phlorizin binding were found to be sigmoidal functions of sodium concentration. A Hill analysis of these data was consistent with a sodium:phlorizin stoichiometry of 2:1 in good agreement with the sodium:glucose stoichiometry already established in these cells. Phlorizin dissociation was also found to be sodium-dependent. On the basis of the phlorizin binding data presented here, a number of models of the binding of phlorizin and sodium to the transporter can be excluded. An analysis of a random binding model consistent with the data is presented. The significance of the LLC-PK1 sodium-dependent D-glucose transporter as a model system for related renal and intestinal transporters is discussed. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/3335496/High_affinity_phlorizin_binding_to_the_LLC-PK1_cells_exhibits_a_sodium:phlorizin_stoichiometry_of_2:1 L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=3335496 DB - PRIME DP - Unbound Medicine ER -