Tags

Type your tag names separated by a space and hit enter

Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro.
Carbohydr Polym. 2021 Feb 15; 254:117251.CP

Abstract

The research described here presents data on the effect of galactans of red algae, carrageenans (λ/μ/ν-, κ-, κ/β-, and ι/κ-types), and agar on complement system activation in normal human serum. The experiments were based on well surfaces coated with triggering agents for binding initiating complement components -C3 and C4. The sulfated galactans inhibited C3 binding to lipopolysaccharide with direct dependence on the sulfation degree of polysaccharides. Sulfation degree was also important in carrageenans' capacity to reduce C4 binding to mannan. However, C4 binding to antibodies was considerably activated by carrageenans, especially with 3,6-anhydrogalactose. The gelling carrageenans were able to block antigen binding centers of total serum IgM and with more intensity than non-gelling. No structural characteristics mattered in ameliorating C5 cleavage by plasmin in extrinsic protease complement activation, but λ/μ/ν- and κ/β-carrageenans almost completely inhibited C5 cleavage. Thus, galactans participated in cell surface biology by imitating surface glycans in inhibition of C3 binding and mannose binding lectin, but as to the tthe heclassical pathway these substances stimulated complement, probably due to their structure based on carrabiose.

Authors+Show Affiliations

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok, 690022, Russia. Electronic address: eka9739@gmail.com.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok, 690022, Russia.Medical Association of the Far East Branch of the Russian Academy of Sciences, Vladivostok, St. Kirova, 95, 690022, Russia.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok, 690022, Russia.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok, 690022, Russia.Medical Association of the Far East Branch of the Russian Academy of Sciences, Vladivostok, St. Kirova, 95, 690022, Russia.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok, 690022, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33357847

Citation

Sokolova, E V., et al. "Effect of Red Seaweed Sulfated Galactans On Initial Steps of Complement Activation in Vitro." Carbohydrate Polymers, vol. 254, 2021, p. 117251.
Sokolova EV, Kravchenko AO, Sergeeva NV, et al. Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro. Carbohydr Polym. 2021;254:117251.
Sokolova, E. V., Kravchenko, A. O., Sergeeva, N. V., Kalinovsky, A. I., Glazunov, V. P., Bogdanovich, L. N., & Yermak, I. M. (2021). Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro. Carbohydrate Polymers, 254, 117251. https://doi.org/10.1016/j.carbpol.2020.117251
Sokolova EV, et al. Effect of Red Seaweed Sulfated Galactans On Initial Steps of Complement Activation in Vitro. Carbohydr Polym. 2021 Feb 15;254:117251. PubMed PMID: 33357847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of red seaweed sulfated galactans on initial steps of complement activation in vitro. AU - Sokolova,E V, AU - Kravchenko,A O, AU - Sergeeva,N V, AU - Kalinovsky,A I, AU - Glazunov,V P, AU - Bogdanovich,L N, AU - Yermak,I M, Y1 - 2020/10/21/ PY - 2020/08/12/received PY - 2020/10/07/revised PY - 2020/10/13/accepted PY - 2020/12/28/entrez PY - 2020/12/29/pubmed PY - 2021/4/10/medline KW - Agar KW - Carrageenan KW - Complement KW - Heparin KW - Lipopolysaccharide KW - Plasmin SP - 117251 EP - 117251 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 254 N2 - The research described here presents data on the effect of galactans of red algae, carrageenans (λ/μ/ν-, κ-, κ/β-, and ι/κ-types), and agar on complement system activation in normal human serum. The experiments were based on well surfaces coated with triggering agents for binding initiating complement components -C3 and C4. The sulfated galactans inhibited C3 binding to lipopolysaccharide with direct dependence on the sulfation degree of polysaccharides. Sulfation degree was also important in carrageenans' capacity to reduce C4 binding to mannan. However, C4 binding to antibodies was considerably activated by carrageenans, especially with 3,6-anhydrogalactose. The gelling carrageenans were able to block antigen binding centers of total serum IgM and with more intensity than non-gelling. No structural characteristics mattered in ameliorating C5 cleavage by plasmin in extrinsic protease complement activation, but λ/μ/ν- and κ/β-carrageenans almost completely inhibited C5 cleavage. Thus, galactans participated in cell surface biology by imitating surface glycans in inhibition of C3 binding and mannose binding lectin, but as to the tthe heclassical pathway these substances stimulated complement, probably due to their structure based on carrabiose. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/33357847/Effect_of_red_seaweed_sulfated_galactans_on_initial_steps_of_complement_activation_in_vitro_ DB - PRIME DP - Unbound Medicine ER -