Tags

Type your tag names separated by a space and hit enter

Evaluation of a novel monoclonal antibody-based enzyme immunoassay for detection of Histoplasma antigen in urine of dogs.
J Vet Intern Med. 2021 Jan; 35(1):284-293.JV

Abstract

BACKGROUND

Commercially available, noninvasive testing options for histoplasmosis are limited outside of the United States.

OBJECTIVES

To describe the diagnostic performance of a novel Histoplasma antigen enzyme immunoassay (IM EIA) for the diagnosis of histoplasmosis in dogs.

ANIMALS

Twenty dogs with histoplasmosis, 79 dogs without histoplasmosis, and 11 unclassified dogs providing 202 urine samples.

METHODS

This a prospective study using stored urine samples. Samples were analyzed with the IM EIA and with the commercially available Histoplasma antigen EIA (MV EIA). Dogs were classified based on final proven diagnosis and performance of the IM EIA was described and compared with the MiraVista enzyme immunoassay (MV EIA).

RESULTS

The diagnostic sensitivity (DSe), specificity (DSp), and accuracy (DAc) of the IM EIA were 70% (51%-89%), 99% (97%-100%), and 93% (81%-100%), respectively. The DSe, DSp, and DAc for the MV EIA were 95% (85%-100%), 99% (97%-100%), and 98% (95%-100%), respectively. The area under the receiver operator characteristic curve was significantly smaller for IM EIA (0.87) as compared with MV EIA (0.97, P = .03). This was primarily due to 6 false negative IM EIA results, 4 from dogs with disease localized to the gastrointestinal tract. The MV EIA was positive in 5/6 of these dogs.

CONCLUSIONS AND CLINICAL IMPORTANCE

The IM EIA might be useful for the diagnosis of disseminated histoplasmosis in dogs, but clinical usefulness will be limited in dogs with histoplasmosis localized to the GI tract.

Authors+Show Affiliations

Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, Oklahoma, USA.Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, Oklahoma, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33368653

Citation

Clark, Kristen, and Andrew S. Hanzlicek. "Evaluation of a Novel Monoclonal Antibody-based Enzyme Immunoassay for Detection of Histoplasma Antigen in Urine of Dogs." Journal of Veterinary Internal Medicine, vol. 35, no. 1, 2021, pp. 284-293.
Clark K, Hanzlicek AS. Evaluation of a novel monoclonal antibody-based enzyme immunoassay for detection of Histoplasma antigen in urine of dogs. J Vet Intern Med. 2021;35(1):284-293.
Clark, K., & Hanzlicek, A. S. (2021). Evaluation of a novel monoclonal antibody-based enzyme immunoassay for detection of Histoplasma antigen in urine of dogs. Journal of Veterinary Internal Medicine, 35(1), 284-293. https://doi.org/10.1111/jvim.16006
Clark K, Hanzlicek AS. Evaluation of a Novel Monoclonal Antibody-based Enzyme Immunoassay for Detection of Histoplasma Antigen in Urine of Dogs. J Vet Intern Med. 2021;35(1):284-293. PubMed PMID: 33368653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a novel monoclonal antibody-based enzyme immunoassay for detection of Histoplasma antigen in urine of dogs. AU - Clark,Kristen, AU - Hanzlicek,Andrew S, Y1 - 2020/12/24/ PY - 2020/07/26/received PY - 2020/11/24/revised PY - 2020/12/02/accepted PY - 2020/12/29/pubmed PY - 2020/12/29/medline PY - 2020/12/28/entrez KW - canine KW - fungal KW - invasive fungal infection KW - mycology KW - mycoses KW - mycosis SP - 284 EP - 293 JF - Journal of veterinary internal medicine JO - J Vet Intern Med VL - 35 IS - 1 N2 - BACKGROUND: Commercially available, noninvasive testing options for histoplasmosis are limited outside of the United States. OBJECTIVES: To describe the diagnostic performance of a novel Histoplasma antigen enzyme immunoassay (IM EIA) for the diagnosis of histoplasmosis in dogs. ANIMALS: Twenty dogs with histoplasmosis, 79 dogs without histoplasmosis, and 11 unclassified dogs providing 202 urine samples. METHODS: This a prospective study using stored urine samples. Samples were analyzed with the IM EIA and with the commercially available Histoplasma antigen EIA (MV EIA). Dogs were classified based on final proven diagnosis and performance of the IM EIA was described and compared with the MiraVista enzyme immunoassay (MV EIA). RESULTS: The diagnostic sensitivity (DSe), specificity (DSp), and accuracy (DAc) of the IM EIA were 70% (51%-89%), 99% (97%-100%), and 93% (81%-100%), respectively. The DSe, DSp, and DAc for the MV EIA were 95% (85%-100%), 99% (97%-100%), and 98% (95%-100%), respectively. The area under the receiver operator characteristic curve was significantly smaller for IM EIA (0.87) as compared with MV EIA (0.97, P = .03). This was primarily due to 6 false negative IM EIA results, 4 from dogs with disease localized to the gastrointestinal tract. The MV EIA was positive in 5/6 of these dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: The IM EIA might be useful for the diagnosis of disseminated histoplasmosis in dogs, but clinical usefulness will be limited in dogs with histoplasmosis localized to the GI tract. SN - 1939-1676 UR - https://www.unboundmedicine.com/medline/citation/33368653/Evaluation_of_a_novel_monoclonal_antibody_based_enzyme_immunoassay_for_detection_of_Histoplasma_antigen_in_urine_of_dogs_ L2 - https://doi.org/10.1111/jvim.16006 DB - PRIME DP - Unbound Medicine ER -