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Exploring the alpha-gliadin locus: the 33-mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii.
Plant J. 2021 04; 106(1):86-94.PJ

Abstract

Most alpha-gliadin genes of the Gli-D2 locus on the D genome of hexaploid bread wheat (Triticum aestivum) encode for proteins with epitopes that can trigger coeliac disease (CD), and several contain a 33-mer peptide with six partly overlapping copies of three epitopes, which is regarded as a remarkably potent T-cell stimulator. To increase genetic diversity in the D genome, synthetic hexaploid wheat lines are being made by hybridising accessions of Triticum turgidum (AB genome) and Aegilops tauschii (the progenitor of the D genome). The diversity of alpha-gliadins in A. tauschii has not been studied extensively. We analysed the alpha-gliadin transcriptome of 51 A. tauschii accessions representative of the diversity in A. tauschii. We extracted RNA from developing seeds and performed 454 amplicon sequencing of the first part of the alpha-gliadin genes. The expression profile of allelic variants of the alpha-gliadins was different between accessions, and also between accessions of the Western and Eastern clades of A. tauschii. Generally, both clades expressed many allelic variants not found in bread wheat. In contrast to earlier studies, we detected the 33-mer peptide in some A. tauschii accessions, indicating that it was introduced along with the D genome into bread wheat. In these accessions, transcripts with the 33-mer peptide were present at lower frequencies than in bread wheat varieties. In most A. tauschii accessions, however, the alpha-gliadins do not contain the epitope, and this may be exploited, through synthetic hexaploid wheats, to breed bread wheat varieties with fewer or no coeliac disease epitopes.

Authors+Show Affiliations

Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands. Bioscience, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands. Allergy Consortium Wageningen, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands. Allergy Consortium Wageningen, Droevendaalsesteeg 1, NL-6708 PB Wageningen, the Netherlands.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33369792

Citation

Schaart, Jan G., et al. "Exploring the Alpha-gliadin Locus: the 33-mer Peptide With Six Overlapping Coeliac Disease Epitopes in Triticum Aestivum Is Derived From a Subgroup of Aegilops Tauschii." The Plant Journal : for Cell and Molecular Biology, vol. 106, no. 1, 2021, pp. 86-94.
Schaart JG, Salentijn EMJ, Goryunova SV, et al. Exploring the alpha-gliadin locus: the 33-mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii. Plant J. 2021;106(1):86-94.
Schaart, J. G., Salentijn, E. M. J., Goryunova, S. V., Chidzanga, C., Esselink, D. G., Gosman, N., Bentley, A. R., Gilissen, L. J. W. J., & Smulders, M. J. M. (2021). Exploring the alpha-gliadin locus: the 33-mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii. The Plant Journal : for Cell and Molecular Biology, 106(1), 86-94. https://doi.org/10.1111/tpj.15147
Schaart JG, et al. Exploring the Alpha-gliadin Locus: the 33-mer Peptide With Six Overlapping Coeliac Disease Epitopes in Triticum Aestivum Is Derived From a Subgroup of Aegilops Tauschii. Plant J. 2021;106(1):86-94. PubMed PMID: 33369792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring the alpha-gliadin locus: the 33-mer peptide with six overlapping coeliac disease epitopes in Triticum aestivum is derived from a subgroup of Aegilops tauschii. AU - Schaart,Jan G, AU - Salentijn,Elma M J, AU - Goryunova,Svetlana V, AU - Chidzanga,Charity, AU - Esselink,Danny G, AU - Gosman,Nick, AU - Bentley,Alison R, AU - Gilissen,Luud J W J, AU - Smulders,Marinus J M, Y1 - 2021/02/19/ PY - 2020/12/13/revised PY - 2020/10/06/received PY - 2020/12/17/accepted PY - 2020/12/29/pubmed PY - 2021/8/19/medline PY - 2020/12/28/entrez KW - Aegilops tauschii KW - Triticum aestivum KW - D genome KW - SHW KW - T-cell epitope KW - alpha-gliadin KW - coeliac disease KW - gluten KW - re-synthesised bread wheat KW - synthetic hexaploid wheat SP - 86 EP - 94 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 106 IS - 1 N2 - Most alpha-gliadin genes of the Gli-D2 locus on the D genome of hexaploid bread wheat (Triticum aestivum) encode for proteins with epitopes that can trigger coeliac disease (CD), and several contain a 33-mer peptide with six partly overlapping copies of three epitopes, which is regarded as a remarkably potent T-cell stimulator. To increase genetic diversity in the D genome, synthetic hexaploid wheat lines are being made by hybridising accessions of Triticum turgidum (AB genome) and Aegilops tauschii (the progenitor of the D genome). The diversity of alpha-gliadins in A. tauschii has not been studied extensively. We analysed the alpha-gliadin transcriptome of 51 A. tauschii accessions representative of the diversity in A. tauschii. We extracted RNA from developing seeds and performed 454 amplicon sequencing of the first part of the alpha-gliadin genes. The expression profile of allelic variants of the alpha-gliadins was different between accessions, and also between accessions of the Western and Eastern clades of A. tauschii. Generally, both clades expressed many allelic variants not found in bread wheat. In contrast to earlier studies, we detected the 33-mer peptide in some A. tauschii accessions, indicating that it was introduced along with the D genome into bread wheat. In these accessions, transcripts with the 33-mer peptide were present at lower frequencies than in bread wheat varieties. In most A. tauschii accessions, however, the alpha-gliadins do not contain the epitope, and this may be exploited, through synthetic hexaploid wheats, to breed bread wheat varieties with fewer or no coeliac disease epitopes. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/33369792/Exploring_the_alpha_gliadin_locus:_the_33_mer_peptide_with_six_overlapping_coeliac_disease_epitopes_in_Triticum_aestivum_is_derived_from_a_subgroup_of_Aegilops_tauschii_ L2 - https://doi.org/10.1111/tpj.15147 DB - PRIME DP - Unbound Medicine ER -