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Ecofriendly Synthesis of Silver Nanoparticles by Terrabacter humi sp. nov. and Their Antibacterial Application against Antibiotic-Resistant Pathogens.
Int J Mol Sci. 2020 Dec 21; 21(24)IJ

Abstract

It is essential to develop and discover alternative eco-friendly antibacterial agents due to the emergence of multi-drug-resistant microorganisms. In this study, we isolated and characterized a novel bacterium named Terrabacter humi MAHUQ-38T, utilized for the eco-friendly synthesis of silver nanoparticles (AgNPs) and the synthesized AgNPs were used to control multi-drug-resistant microorganisms. The novel strain was Gram stain positive, strictly aerobic, milky white colored, rod shaped and non-motile. The optimal growth temperature, pH and NaCl concentration were 30 °C, 6.5 and 0%, respectively. Based on 16S rRNA gene sequence, strain MAHUQ-38T belongs to the genus Terrabacter and is most closely related to several Terrabacter type strains (98.2%-98.8%). Terrabacter humi MAHUQ-38T had a genome of 5,156,829 bp long (19 contigs) with 4555 protein-coding genes, 48 tRNA and 5 rRNA genes. The culture supernatant of strain MAHUQ-38T was used for the eco-friendly and facile synthesis of AgNPs. The transmission electron microscopy (TEM) image showed the spherical shape of AgNPs with a size of 6 to 24 nm, and the Fourier transform infrared (FTIR) analysis revealed the functional groups responsible for the synthesis of AgNPs. The synthesized AgNPs exhibited strong anti-bacterial activity against multi-drug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 6.25/50 and 12.5/50 μg/mL, respectively. The AgNPs altered the cell morphology and damaged the cell membrane of pathogens. This study encourages the use of Terrabacter humi for the ecofriendly synthesis of AgNPs to control multi-drug-resistant microorganisms.

Authors+Show Affiliations

Department of Food Science and Biotechnology, Gachon University, Seongnam 461-701, Korea.Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Korea.Department of Biotechnology, Hankyong National University, Anseong 17546, Korea.Department of Food Science and Technology, Sejong University, Seoul 143-747, Korea.Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea.Department of Oriental Medicinal Material Processing, Kyung Hee University, Yongin 17104, Korea.Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33371231

Citation

Akter, Shahina, et al. "Ecofriendly Synthesis of Silver Nanoparticles By Terrabacter Humi Sp. Nov. and Their Antibacterial Application Against Antibiotic-Resistant Pathogens." International Journal of Molecular Sciences, vol. 21, no. 24, 2020.
Akter S, Lee SY, Siddiqi MZ, et al. Ecofriendly Synthesis of Silver Nanoparticles by Terrabacter humi sp. nov. and Their Antibacterial Application against Antibiotic-Resistant Pathogens. Int J Mol Sci. 2020;21(24).
Akter, S., Lee, S. Y., Siddiqi, M. Z., Balusamy, S. R., Ashrafudoulla, M., Rupa, E. J., & Huq, M. A. (2020). Ecofriendly Synthesis of Silver Nanoparticles by Terrabacter humi sp. nov. and Their Antibacterial Application against Antibiotic-Resistant Pathogens. International Journal of Molecular Sciences, 21(24). https://doi.org/10.3390/ijms21249746
Akter S, et al. Ecofriendly Synthesis of Silver Nanoparticles By Terrabacter Humi Sp. Nov. and Their Antibacterial Application Against Antibiotic-Resistant Pathogens. Int J Mol Sci. 2020 Dec 21;21(24) PubMed PMID: 33371231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ecofriendly Synthesis of Silver Nanoparticles by Terrabacter humi sp. nov. and Their Antibacterial Application against Antibiotic-Resistant Pathogens. AU - Akter,Shahina, AU - Lee,Sun-Young, AU - Siddiqi,Muhammad Zubair, AU - Balusamy,Sri Renukadevi, AU - Ashrafudoulla,Md, AU - Rupa,Esrat Jahan, AU - Huq,Md Amdadul, Y1 - 2020/12/21/ PY - 2020/11/21/received PY - 2020/12/16/revised PY - 2020/12/17/accepted PY - 2020/12/29/entrez PY - 2020/12/30/pubmed PY - 2020/12/30/medline KW - AgNPs KW - Terrabacter humi MAHUQ-38T KW - eco-friendly synthesis KW - multi-drug-resistant microorganisms JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 24 N2 - It is essential to develop and discover alternative eco-friendly antibacterial agents due to the emergence of multi-drug-resistant microorganisms. In this study, we isolated and characterized a novel bacterium named Terrabacter humi MAHUQ-38T, utilized for the eco-friendly synthesis of silver nanoparticles (AgNPs) and the synthesized AgNPs were used to control multi-drug-resistant microorganisms. The novel strain was Gram stain positive, strictly aerobic, milky white colored, rod shaped and non-motile. The optimal growth temperature, pH and NaCl concentration were 30 °C, 6.5 and 0%, respectively. Based on 16S rRNA gene sequence, strain MAHUQ-38T belongs to the genus Terrabacter and is most closely related to several Terrabacter type strains (98.2%-98.8%). Terrabacter humi MAHUQ-38T had a genome of 5,156,829 bp long (19 contigs) with 4555 protein-coding genes, 48 tRNA and 5 rRNA genes. The culture supernatant of strain MAHUQ-38T was used for the eco-friendly and facile synthesis of AgNPs. The transmission electron microscopy (TEM) image showed the spherical shape of AgNPs with a size of 6 to 24 nm, and the Fourier transform infrared (FTIR) analysis revealed the functional groups responsible for the synthesis of AgNPs. The synthesized AgNPs exhibited strong anti-bacterial activity against multi-drug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 6.25/50 and 12.5/50 μg/mL, respectively. The AgNPs altered the cell morphology and damaged the cell membrane of pathogens. This study encourages the use of Terrabacter humi for the ecofriendly synthesis of AgNPs to control multi-drug-resistant microorganisms. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/33371231/Ecofriendly_Synthesis_of_Silver_Nanoparticles_by_Terrabacter_humi_sp__nov__and_Their_Antibacterial_Application_against_Antibiotic_Resistant_Pathogens_ L2 - https://www.mdpi.com/resolver?pii=ijms21249746 DB - PRIME DP - Unbound Medicine ER -