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Preliminary validation of the use of IgG antibody response to Anopheles gSG6-p1 salivary peptide to assess human exposure to malaria vector bites in two endemic areas of Cameroon in Central Africa.
PLoS One. 2020; 15(12):e0242510.Plos

Abstract

The specific immune response to the Anopheles salivary peptide could be a pertinent and complementary tool to assess the risk of malaria transmission and the effectiveness of vector control strategies. This study aimed to obtain first reliable data on the current state of the Anopheles gSG6-P1 biomarker for assess the level of exposure to Anopheles bites in high malaria endemic areas in Cameroon. Blood smears were collected from people living in the neighborhoods of Youpwe (suburban area, continental) and Manoka (rural area, Island), both areas in the coastal region of Cameroon. Malaria infection was determined using thick blood smear microscopy, whereas the level of specific IgG response to gSG-P1 peptide was assessed by enzyme-linked immunosorbent assay from the dried blood spots. Of 266 (153 from Youpwe, 113 from Manoka) malaria endemic residents (mean age: 22.8±19.8 years, age range: 6 months-94 years, male/female sex ratio: 1/1.2, with Manoka mean age: 23.71±20.53, male/female sex ratio:1/1.13 and Youpwe mean age: 22.12±19.22, male/female sex ratio 1/0.67) randomly included in the study, Plasmodium infection prevalence was significantly higher in Manoka than in Youpwe (64.6% vs 12,4%, p = 0.0001). The anti-gSG6-P1 IgG response showed a high inter-individual heterogeneity and was significantly higher among individuals from Manoka than those from Youpwe (p = 0.023). Malaria infected individuals presented a higher anti-gSG6-P1 IgG antibody response than non-infected (p = 0.0004). No significant difference in the level of specific IgG response to gSG-P1 was observed according to long lasting insecticidal nets use. Taken together, the data revealed that human IgG antibody response to Anopheles gSG-P1 salivary peptide could be also used to assess human exposure to malaria vectors in Central African region. This finding strengthens the relevance of this candidate biomarker to be used for measuring human exposure to malaria vectors worldwide.

Authors+Show Affiliations

Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon. Department of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea, Cameroon.Centre for Research in Infectious Diseases, Yaounde, Cameroon.Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon. Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaounde, Yaounde, Cameroon.Parasitology and Entomology Research Unit, Department of Animal Biology and Organisms, Faculty of Sciences, University of Douala, Douala, Cameroon.Department of hematology, Centre Pasteur of Cameroon, Yaoundé, Cameroon.Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon. Parasitology and Entomology Research Unit, Department of Animal Biology and Organisms, Faculty of Sciences, University of Douala, Douala, Cameroon.Biological Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon.Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon. Laboratory of Parasitology, Mycology and Virology, Postgraduate Training Unit for Health Sciences, Postgraduate School for Pure and Applied Sciences, University of Douala, Douala, Cameroon.Biological Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon. Organisation de Coordination pour la Lutte contre les Endemies en Afrique Central, Yaounde, Cameroon.Department of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea, Cameroon.Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon.Malaria Research Unit, Centre Pasteur Cameroon, Yaounde, Cameroon. Biological Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon. Laboratory of Parasitology, Mycology and Virology, Postgraduate Training Unit for Health Sciences, Postgraduate School for Pure and Applied Sciences, University of Douala, Douala, Cameroon.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

33382730

Citation

Cheteug, Glwadys, et al. "Preliminary Validation of the Use of IgG Antibody Response to Anopheles gSG6-p1 Salivary Peptide to Assess Human Exposure to Malaria Vector Bites in Two Endemic Areas of Cameroon in Central Africa." PloS One, vol. 15, no. 12, 2020, pp. e0242510.
Cheteug G, Elanga-Ndille E, Donkeu C, et al. Preliminary validation of the use of IgG antibody response to Anopheles gSG6-p1 salivary peptide to assess human exposure to malaria vector bites in two endemic areas of Cameroon in Central Africa. PLoS One. 2020;15(12):e0242510.
Cheteug, G., Elanga-Ndille, E., Donkeu, C., Ekoko, W., Oloume, M., Essangui, E., Nwane, P., NSango, S. E., Etang, J., Wanji, S., Ayong, L., & Eboumbou Moukoko, C. E. (2020). Preliminary validation of the use of IgG antibody response to Anopheles gSG6-p1 salivary peptide to assess human exposure to malaria vector bites in two endemic areas of Cameroon in Central Africa. PloS One, 15(12), e0242510. https://doi.org/10.1371/journal.pone.0242510
Cheteug G, et al. Preliminary Validation of the Use of IgG Antibody Response to Anopheles gSG6-p1 Salivary Peptide to Assess Human Exposure to Malaria Vector Bites in Two Endemic Areas of Cameroon in Central Africa. PLoS One. 2020;15(12):e0242510. PubMed PMID: 33382730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary validation of the use of IgG antibody response to Anopheles gSG6-p1 salivary peptide to assess human exposure to malaria vector bites in two endemic areas of Cameroon in Central Africa. AU - Cheteug,Glwadys, AU - Elanga-Ndille,Emmanuel, AU - Donkeu,Christiane, AU - Ekoko,Wolfgang, AU - Oloume,Martine, AU - Essangui,Estelle, AU - Nwane,Philippe, AU - NSango,Sandrine Eveline, AU - Etang,Josiane, AU - Wanji,Samuel, AU - Ayong,Lawrence, AU - Eboumbou Moukoko,Carole Else, Y1 - 2020/12/31/ PY - 2020/04/03/received PY - 2020/11/03/accepted PY - 2020/12/31/entrez PY - 2021/1/1/pubmed PY - 2021/1/1/medline SP - e0242510 EP - e0242510 JF - PloS one JO - PLoS One VL - 15 IS - 12 N2 - The specific immune response to the Anopheles salivary peptide could be a pertinent and complementary tool to assess the risk of malaria transmission and the effectiveness of vector control strategies. This study aimed to obtain first reliable data on the current state of the Anopheles gSG6-P1 biomarker for assess the level of exposure to Anopheles bites in high malaria endemic areas in Cameroon. Blood smears were collected from people living in the neighborhoods of Youpwe (suburban area, continental) and Manoka (rural area, Island), both areas in the coastal region of Cameroon. Malaria infection was determined using thick blood smear microscopy, whereas the level of specific IgG response to gSG-P1 peptide was assessed by enzyme-linked immunosorbent assay from the dried blood spots. Of 266 (153 from Youpwe, 113 from Manoka) malaria endemic residents (mean age: 22.8±19.8 years, age range: 6 months-94 years, male/female sex ratio: 1/1.2, with Manoka mean age: 23.71±20.53, male/female sex ratio:1/1.13 and Youpwe mean age: 22.12±19.22, male/female sex ratio 1/0.67) randomly included in the study, Plasmodium infection prevalence was significantly higher in Manoka than in Youpwe (64.6% vs 12,4%, p = 0.0001). The anti-gSG6-P1 IgG response showed a high inter-individual heterogeneity and was significantly higher among individuals from Manoka than those from Youpwe (p = 0.023). Malaria infected individuals presented a higher anti-gSG6-P1 IgG antibody response than non-infected (p = 0.0004). No significant difference in the level of specific IgG response to gSG-P1 was observed according to long lasting insecticidal nets use. Taken together, the data revealed that human IgG antibody response to Anopheles gSG-P1 salivary peptide could be also used to assess human exposure to malaria vectors in Central African region. This finding strengthens the relevance of this candidate biomarker to be used for measuring human exposure to malaria vectors worldwide. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/33382730/Preliminary_validation_of_the_use_of_IgG_antibody_response_to_Anopheles_gSG6_p1_salivary_peptide_to_assess_human_exposure_to_malaria_vector_bites_in_two_endemic_areas_of_Cameroon_in_Central_Africa_ L2 - https://dx.plos.org/10.1371/journal.pone.0242510 DB - PRIME DP - Unbound Medicine ER -