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Single-cell RNA sequencing in vision research: Insights into human retinal health and disease.
Prog Retin Eye Res. 2021 Jul; 83:100934.PR

Abstract

Gene expression provides valuable insight into cell function. As such, vision researchers have frequently employed gene expression studies to better understand retinal physiology and disease. With the advent of single-cell RNA sequencing, expression experiments provide an unparalleled resolution of information. Instead of studying aggregated gene expression across all cells in a heterogenous tissue, single-cell technology maps RNA to an individual cell, which facilitates grouping of retinal and choroidal cell types for further study. Single-cell RNA sequencing has been quickly adopted by both basic and translational vision researchers, and single-cell level gene expression has been studied in the visual systems of animal models, retinal organoids, and primary human retina, RPE, and choroid. These experiments have generated detailed atlases of gene expression and identified new retinal cell types. Likewise, single-cell RNA sequencing investigations have characterized how gene expression changes in the setting of many retinal diseases, including how choroidal endothelial cells are altered in age-related macular degeneration. In addition, this technology has allowed vision researchers to discover drivers of retinal development and model rare retinal diseases with induced pluripotent stem cells. In this review, we will overview the growing number of single-cell RNA sequencing studies in the field of vision research. We will summarize experimental considerations for designing single-cell RNA sequencing experiments and highlight important advancements in retinal, RPE, choroidal, and retinal organoid biology driven by this technology. Finally, we generalize these findings to genes involved in retinal degeneration and outline the future of single-cell expression experiments in studying retinal disease.

Authors+Show Affiliations

Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA.Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA.Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA.Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA.Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA.Departments of Ophthalmology and Visual Sciences, The University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Institute for Vision Research, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: robert-mullins@uiowa.edu.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

33383180

Citation

Voigt, Andrew P., et al. "Single-cell RNA Sequencing in Vision Research: Insights Into Human Retinal Health and Disease." Progress in Retinal and Eye Research, vol. 83, 2021, p. 100934.
Voigt AP, Mullin NK, Stone EM, et al. Single-cell RNA sequencing in vision research: Insights into human retinal health and disease. Prog Retin Eye Res. 2021;83:100934.
Voigt, A. P., Mullin, N. K., Stone, E. M., Tucker, B. A., Scheetz, T. E., & Mullins, R. F. (2021). Single-cell RNA sequencing in vision research: Insights into human retinal health and disease. Progress in Retinal and Eye Research, 83, 100934. https://doi.org/10.1016/j.preteyeres.2020.100934
Voigt AP, et al. Single-cell RNA Sequencing in Vision Research: Insights Into Human Retinal Health and Disease. Prog Retin Eye Res. 2021;83:100934. PubMed PMID: 33383180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-cell RNA sequencing in vision research: Insights into human retinal health and disease. AU - Voigt,Andrew P, AU - Mullin,Nathaniel K, AU - Stone,Edwin M, AU - Tucker,Budd A, AU - Scheetz,Todd E, AU - Mullins,Robert F, Y1 - 2020/12/28/ PY - 2020/08/30/received PY - 2020/11/27/revised PY - 2020/12/01/accepted PY - 2022/07/01/pmc-release PY - 2021/1/1/pubmed PY - 2021/1/1/medline PY - 2020/12/31/entrez KW - Choriocapillaris KW - Choroid KW - Endothelial KW - RNA KW - RPE KW - Retina KW - Single-cell KW - Transcriptome SP - 100934 EP - 100934 JF - Progress in retinal and eye research JO - Prog Retin Eye Res VL - 83 N2 - Gene expression provides valuable insight into cell function. As such, vision researchers have frequently employed gene expression studies to better understand retinal physiology and disease. With the advent of single-cell RNA sequencing, expression experiments provide an unparalleled resolution of information. Instead of studying aggregated gene expression across all cells in a heterogenous tissue, single-cell technology maps RNA to an individual cell, which facilitates grouping of retinal and choroidal cell types for further study. Single-cell RNA sequencing has been quickly adopted by both basic and translational vision researchers, and single-cell level gene expression has been studied in the visual systems of animal models, retinal organoids, and primary human retina, RPE, and choroid. These experiments have generated detailed atlases of gene expression and identified new retinal cell types. Likewise, single-cell RNA sequencing investigations have characterized how gene expression changes in the setting of many retinal diseases, including how choroidal endothelial cells are altered in age-related macular degeneration. In addition, this technology has allowed vision researchers to discover drivers of retinal development and model rare retinal diseases with induced pluripotent stem cells. In this review, we will overview the growing number of single-cell RNA sequencing studies in the field of vision research. We will summarize experimental considerations for designing single-cell RNA sequencing experiments and highlight important advancements in retinal, RPE, choroidal, and retinal organoid biology driven by this technology. Finally, we generalize these findings to genes involved in retinal degeneration and outline the future of single-cell expression experiments in studying retinal disease. SN - 1873-1635 UR - https://www.unboundmedicine.com/medline/citation/33383180/Single-cell_RNA_sequencing_in_vision_research:_Insights_into_human_retinal_health_and_disease. L2 - https://linkinghub.elsevier.com/retrieve/pii/S1350-9462(20)30106-3 DB - PRIME DP - Unbound Medicine ER -
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