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Calcium-hydrogen sulfide crosstalk during K[+]-deficient NaCl stress operates through regulation of Na[+]/H[+] antiport and antioxidative defense system in mung bean roots.
Plant Physiol Biochem. 2021 Feb; 159:211-225.PP

Abstract

Present investigation reports the role of calcium (Ca[2+]) and hydrogen sulfide (H2S) crosstalk associated with Vigna radiata seedlings subjected to K[+] deficient conditions under short-term (24 h) and long-term (72 h) NaCl stress. Perusal of the data reveals that under short-term NaCl stress an initial decline in K[+] level led to the elevation in Ca[2+] and H2S levels along with improvement in antioxidant system and reduction in reactive oxygen species (ROS) production. Under long-term NaCl stress a further decline in K[+] content was deleterious that led to a lower K[+]/Na[+] ratio. This was followed by reduction in antioxidant system along with excessive accumulation of ROS and methylglyoxal content, and increased membrane damage. However, supplementation of the seedling roots with Ca[2+] enhanced biosynthesis of H2S through enhancing cysteine pool. The present findings suggest that synergistic action of Ca[2+] and H2S induced the activity of H[+]-ATPase that created H[+] gradient which in turn induced Na[+]/H[+] antiport system that accelerated K[+] influx and Na[+] efflux. All of these together contributed to a higher K[+]/Na[+] ratio, activation of antioxidative defense system, and maintenance of redox homeostasis and membrane integrity in Ca[2+]-supplemented stressed seedlings. Role of Ca[2+] and H2S in the regulation of Na[+]/H[+] antiport system was validated by the use of sodium orthovanadate (plasma membrane H[+]-ATPase inhibitor), tetraethylammonium chloride (K[+] channel blocker), and amiloride (Na[+]/H[+] antiporter inhibitor). Application of Ca[2+]-chelator EGTA (ethylene glycol-bis(b-aminoethylether)-N,N,N',N'-tetraacetic acid) and H2S scavenger hypotaurine abolished the effect of Ca[2+], suggesting the involvement of Ca[2+] and H2S in the alleviation of NaCl stress. Moreover, use of EGTA and HT also substantiates the downstream functioning of H2S during Ca[2+]-mediated regulation of plant adaptive responses to NaCl stress. To sum up, present findings reveal the association of Ca[2+] and H2S signaling in the regulation of ion homeostasis and antioxidant defense during K[+]-deficient NaCl stress.

Authors+Show Affiliations

Department of Biology, Environmental Research Unit, College of Haql, University of Tabuk, Tabuk, 71491, Saudi Arabia. Electronic address: mo.khan@ut.edu.sa.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia. Electronic address: mhsiddiqui@ksu.edu.sa.Department of Botany, Jangipur College, University of Kalyani, West Bengal, 742213, India.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 2455, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33385704

Citation

Khan, M Nasir, et al. "Calcium-hydrogen Sulfide Crosstalk During K[+]-deficient NaCl Stress Operates Through Regulation of Na[+]/H[+] Antiport and Antioxidative Defense System in Mung Bean Roots." Plant Physiology and Biochemistry : PPB, vol. 159, 2021, pp. 211-225.
Khan MN, Siddiqui MH, Mukherjee S, et al. Calcium-hydrogen sulfide crosstalk during K[+]-deficient NaCl stress operates through regulation of Na[+]/H[+] antiport and antioxidative defense system in mung bean roots. Plant Physiol Biochem. 2021;159:211-225.
Khan, M. N., Siddiqui, M. H., Mukherjee, S., Alamri, S., Al-Amri, A. A., Alsubaie, Q. D., Al-Munqedhi, B. M. A., & Ali, H. M. (2021). Calcium-hydrogen sulfide crosstalk during K[+]-deficient NaCl stress operates through regulation of Na[+]/H[+] antiport and antioxidative defense system in mung bean roots. Plant Physiology and Biochemistry : PPB, 159, 211-225. https://doi.org/10.1016/j.plaphy.2020.11.055
Khan MN, et al. Calcium-hydrogen Sulfide Crosstalk During K[+]-deficient NaCl Stress Operates Through Regulation of Na[+]/H[+] Antiport and Antioxidative Defense System in Mung Bean Roots. Plant Physiol Biochem. 2021;159:211-225. PubMed PMID: 33385704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcium-hydrogen sulfide crosstalk during K[+]-deficient NaCl stress operates through regulation of Na[+]/H[+] antiport and antioxidative defense system in mung bean roots. AU - Khan,M Nasir, AU - Siddiqui,Manzer H, AU - Mukherjee,Soumya, AU - Alamri,Saud, AU - Al-Amri,Abdullah A, AU - Alsubaie,Qasi D, AU - Al-Munqedhi,Bander M A, AU - Ali,Hayssam M, Y1 - 2020/12/08/ PY - 2020/11/01/received PY - 2020/11/24/accepted PY - 2021/1/2/pubmed PY - 2021/2/17/medline PY - 2021/1/1/entrez KW - Ascorbate-glutathione cycle KW - Calcium signaling KW - Hydrogen sulfide KW - Na(+)/H(+) antiport KW - Proline KW - Redox homeostasis SP - 211 EP - 225 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 159 N2 - Present investigation reports the role of calcium (Ca[2+]) and hydrogen sulfide (H2S) crosstalk associated with Vigna radiata seedlings subjected to K[+] deficient conditions under short-term (24 h) and long-term (72 h) NaCl stress. Perusal of the data reveals that under short-term NaCl stress an initial decline in K[+] level led to the elevation in Ca[2+] and H2S levels along with improvement in antioxidant system and reduction in reactive oxygen species (ROS) production. Under long-term NaCl stress a further decline in K[+] content was deleterious that led to a lower K[+]/Na[+] ratio. This was followed by reduction in antioxidant system along with excessive accumulation of ROS and methylglyoxal content, and increased membrane damage. However, supplementation of the seedling roots with Ca[2+] enhanced biosynthesis of H2S through enhancing cysteine pool. The present findings suggest that synergistic action of Ca[2+] and H2S induced the activity of H[+]-ATPase that created H[+] gradient which in turn induced Na[+]/H[+] antiport system that accelerated K[+] influx and Na[+] efflux. All of these together contributed to a higher K[+]/Na[+] ratio, activation of antioxidative defense system, and maintenance of redox homeostasis and membrane integrity in Ca[2+]-supplemented stressed seedlings. Role of Ca[2+] and H2S in the regulation of Na[+]/H[+] antiport system was validated by the use of sodium orthovanadate (plasma membrane H[+]-ATPase inhibitor), tetraethylammonium chloride (K[+] channel blocker), and amiloride (Na[+]/H[+] antiporter inhibitor). Application of Ca[2+]-chelator EGTA (ethylene glycol-bis(b-aminoethylether)-N,N,N',N'-tetraacetic acid) and H2S scavenger hypotaurine abolished the effect of Ca[2+], suggesting the involvement of Ca[2+] and H2S in the alleviation of NaCl stress. Moreover, use of EGTA and HT also substantiates the downstream functioning of H2S during Ca[2+]-mediated regulation of plant adaptive responses to NaCl stress. To sum up, present findings reveal the association of Ca[2+] and H2S signaling in the regulation of ion homeostasis and antioxidant defense during K[+]-deficient NaCl stress. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/33385704/Calcium_hydrogen_sulfide_crosstalk_during_K[+]_deficient_NaCl_stress_operates_through_regulation_of_Na[+]/H[+]_antiport_and_antioxidative_defense_system_in_mung_bean_roots_ DB - PRIME DP - Unbound Medicine ER -