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The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms.
Int J Med Mushrooms. 2020; 22(9):869-883.IJ

Abstract

Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively). UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 nm for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, 0.97 μg/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 μg/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 μg/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection.

Authors+Show Affiliations

Institute for Food Technology and Biochemistry, University of Belgrade Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia.Innovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11060 Belgrade, Serbia.Institute for Food Technology and Biochemistry, University of Belgrade Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia.Institute for Food Technology and Biochemistry, University of Belgrade Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia.Plant Research International, Wageningen University and Research, Bornsesteeg 1, 6708PD, Wageningen, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33389853

Citation

Klaus, Anita, et al. "The Antimicrobial Activities of Silver Nanoparticles Synthesized From Medicinal Mushrooms." International Journal of Medicinal Mushrooms, vol. 22, no. 9, 2020, pp. 869-883.
Klaus A, Petrovic P, Vunduk J, et al. The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms. Int J Med Mushrooms. 2020;22(9):869-883.
Klaus, A., Petrovic, P., Vunduk, J., Pavlovic, V., & Van Griensven, L. J. L. D. (2020). The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms. International Journal of Medicinal Mushrooms, 22(9), 869-883. https://doi.org/10.1615/IntJMedMushrooms.2020035988
Klaus A, et al. The Antimicrobial Activities of Silver Nanoparticles Synthesized From Medicinal Mushrooms. Int J Med Mushrooms. 2020;22(9):869-883. PubMed PMID: 33389853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Antimicrobial Activities of Silver Nanoparticles Synthesized from Medicinal Mushrooms. AU - Klaus,Anita, AU - Petrovic,Predrag, AU - Vunduk,Jovana, AU - Pavlovic,Vladimir, AU - Van Griensven,Leonardus Johannes Lambertus Donatus, PY - 2021/1/3/entrez PY - 2021/1/4/pubmed PY - 2021/7/17/medline SP - 869 EP - 883 JF - International journal of medicinal mushrooms JO - Int J Med Mushrooms VL - 22 IS - 9 N2 - Crude polysaccharide extracts derived from Agaricus bisporus (A), A. brasiliensis (B), and Phellinus linteus (P) were used for the synthesis of silver nanoparticles (AgNPs, labeled AAgNP, BAgNP, PAgNP, respectively). UV and FT-IR spectra, size distribution, and zeta potential of the respective particles were recorded, and TEM and SEM-EDS characterization was done. UV-Vis spectroscopy showed maximum absorbance at 415 nm for A, at 400 nm for P, and at 420 nm for B. SEM-EDS analysis showed BAgNP to consist of almost 90% of silver, while silver content in AAgNP and PAgNP was much lower, 46.5 and 52.3%, respectively. Individual extracts as well as the Ag-NPs were tested against a panel of clinically isolated and ATCC derived pathogens. In almost all cases minimum inhibitory concentration (MIC) was significantly lower than those of antibiotics, suggesting up to 100 times more effectivity. BAgNP, PAgNP, and AAgNP appeared very active against Pseudomonas aeruginosa ATCC 27853 (MIC: 0.19, 0.19, 0.97 μg/mL, respectively). The same nanoparticles were also very effective toward Candida albicans ATCC 10231 (MIC: 0.39, 0.78, 0.97 μg/mL, respectively). Our study indicates that the bactericidal effect of PAgNP, AAgNP, BAgNP on E. coli 25922 ATCC (MBC: 1.56, 0.97, 6.25 μg/mL, respectively) is significantly more pronounced than that of amoxicillin. A pure solution of colloidal silver nanoparticles showed significantly weaker microbiostatic/microbiocidal potential than all tested mushroom extracts' AgNPs, as well as amoxicillin. Silver nanoparticles made with extracts of A, B, and P seem welcome as an addition to the inventory of antimicrobial compounds used in clinical medicine against bacterial and yeast infection. SN - 1940-4344 UR - https://www.unboundmedicine.com/medline/citation/33389853/The_Antimicrobial_Activities_of_Silver_Nanoparticles_Synthesized_from_Medicinal_Mushrooms. L2 - http://www.begellhouse.com/journals/708ae68d64b17c52,358d19d227c8633b,3bdfbdb5395cc18c.html DB - PRIME DP - Unbound Medicine ER -