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Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine.
Chem Pharm Bull (Tokyo). 2021; 69(1):18-25.CP

Abstract

Mogroside V is one of the characteristic and effective components of luohanguo extract, a food additive used as a sweetener in Japan as per Japan's Standards and Specifications for Food Additives (JSFA; 9th ed.). JSFA stipulates that the quantitative determination for mogroside V content in luohanguo extract applies HPLC using analytical standard mogroside V. However, no mogroside V reagents with proven purities are commercially available. Therefore the current JSFA determination method is not particularly suited for daily quality control operations involving luohanguo extract. In this study, we applied an alternative quantitative method using a single reference with relative molar sensitivity (RMS). It was possible to calculate the accurate RMS by an offline combination of 1H-quantitative NMR spectroscopy (1H-qNMR) and an HPLC/variable-wavelength detector (VWD). Using the RMS of mogroside V to a commercial certified reference material grade caffeine, the mogroside V contents in luohanguo extracts could be determined using HPLC/VWD without analytical standard mogroside V. There was no significant difference between the mogroside V contents in luohanguo extracts determined using the method employing single-reference caffeine with the RMS and using the JSFA method. The absolute calibration curve for the latter was prepared using an analytical standard mogroside V whose purity was determined by 1H-qNMR. These results demonstrate that our proposed method using a single reference with RMS is suitable for quantitative determination of mogroside V in luohanguo extract and can be used as an alternative method to the current assay method in JSFA.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Masumoto N
Division of Food Additives, National Institute of Health Sciences.
Ishizuki K
Division of Food Additives, National Institute of Health Sciences.
Nishizaki Y
Division of Food Additives, National Institute of Health Sciences.
Ohtsuki T
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University.
Kuroe M
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology.
Yamazaki T
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology.
Numata M
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology.
Matsufuji H
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University.
Sugimoto N
Division of Food Additives, National Institute of Health Sciences.
Sato K
Division of Food Additives, National Institute of Health Sciences.

MeSH

CaffeineChromatography, High Pressure LiquidCucurbitaceaeFood AdditivesJapanMagnetic Resonance SpectroscopyPlant ExtractsQuality ControlTriterpenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33390516

Citation

Masumoto, Naoko, et al. "Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine." Chemical & Pharmaceutical Bulletin, vol. 69, no. 1, 2021, pp. 18-25.
Masumoto N, Ishizuki K, Nishizaki Y, et al. Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine. Chem Pharm Bull (Tokyo). 2021;69(1):18-25.
Masumoto, N., Ishizuki, K., Nishizaki, Y., Ohtsuki, T., Kuroe, M., Yamazaki, T., Numata, M., Matsufuji, H., Sugimoto, N., & Sato, K. (2021). Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine. Chemical & Pharmaceutical Bulletin, 69(1), 18-25. https://doi.org/10.1248/cpb.c20-00245
Masumoto N, et al. Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine. Chem Pharm Bull (Tokyo). 2021;69(1):18-25. PubMed PMID: 33390516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Mogroside V in Luohanguo Extract for Daily Quality Control Operation Using Relative Molar Sensitivity to Single-Reference Caffeine. AU - Masumoto,Naoko, AU - Ishizuki,Kyoko, AU - Nishizaki,Yuzo, AU - Ohtsuki,Takashi, AU - Kuroe,Miho, AU - Yamazaki,Taichi, AU - Numata,Masahiko, AU - Matsufuji,Hiroshi, AU - Sugimoto,Naoki, AU - Sato,Kyoko, PY - 2021/1/4/entrez PY - 2021/1/5/pubmed PY - 2021/6/3/medline KW - 1H-quantitative NMR spectroscopy KW - caffeine KW - luohanguo extract KW - mogroside V KW - relative molar sensitivity (RMS) KW - single-reference HPLC SP - 18 EP - 25 JF - Chemical & pharmaceutical bulletin JO - Chem Pharm Bull (Tokyo) VL - 69 IS - 1 N2 - Mogroside V is one of the characteristic and effective components of luohanguo extract, a food additive used as a sweetener in Japan as per Japan's Standards and Specifications for Food Additives (JSFA; 9th ed.). JSFA stipulates that the quantitative determination for mogroside V content in luohanguo extract applies HPLC using analytical standard mogroside V. However, no mogroside V reagents with proven purities are commercially available. Therefore the current JSFA determination method is not particularly suited for daily quality control operations involving luohanguo extract. In this study, we applied an alternative quantitative method using a single reference with relative molar sensitivity (RMS). It was possible to calculate the accurate RMS by an offline combination of 1H-quantitative NMR spectroscopy (1H-qNMR) and an HPLC/variable-wavelength detector (VWD). Using the RMS of mogroside V to a commercial certified reference material grade caffeine, the mogroside V contents in luohanguo extracts could be determined using HPLC/VWD without analytical standard mogroside V. There was no significant difference between the mogroside V contents in luohanguo extracts determined using the method employing single-reference caffeine with the RMS and using the JSFA method. The absolute calibration curve for the latter was prepared using an analytical standard mogroside V whose purity was determined by 1H-qNMR. These results demonstrate that our proposed method using a single reference with RMS is suitable for quantitative determination of mogroside V in luohanguo extract and can be used as an alternative method to the current assay method in JSFA. SN - 1347-5223 UR - https://www.unboundmedicine.com/medline/citation/33390516/Determination_of_Mogroside_V_in_Luohanguo_Extract_for_Daily_Quality_Control_Operation_Using_Relative_Molar_Sensitivity_to_Single_Reference_Caffeine_ DB - PRIME DP - Unbound Medicine ER -