Tags

Type your tag names separated by a space and hit enter

Detection of ɣ-hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) in alcoholic beverages via total vaporization solid-phase microextraction (TV-SPME) and gas chromatography-mass spectrometry.
J Forensic Sci. 2021 May; 66(3):846-853.JF

Abstract

Total Vaporization Solid-Phase Microextraction (TV-SPME) relies on the same technique as standard SPME but completely vaporizes a sample extract, and analytes are sorbed directly from the vapor phase. On-fiber derivatization may also be performed using TV-SPME, where the fiber is first exposed to the headspace of a vial containing the derivatization agent, then exposed to a new vial containing the sample. ɣ-Hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) are drugs of concern in that they may be used in drug facilitated sexual assault by surreptitiously spiking them into a victim's beverage. These drugs cause sedation, memory loss, and are difficult to detect in biological samples. One challenge in their analysis is that they can interconvert in aqueous samples, which was demonstrated in samples allowed to stand at room temperature for long periods. A volume study of GBL in water was performed with volumes ranging from 1 to 10,000 µl to compare the efficacy of TV-SPME, headspace SPME, and immersion SPME. Lastly, water, beer, wine, liquor, and mixed drinks were spiked with either GHB or GBL with realistic concentrations (mg/ml) and microliter quantities were analyzed using a TV-SPME Gas Chromatography-Mass Spectrometry method. The GBL volume study demonstrated an increased sensitivity in GBL detection when TV-SPME was utilized. Additionally, GHB and GBL were identified in various beverages at realistic concentrations. Overall, TV-SPME is beneficial because it requires no sample preparation and uses smaller sample volumes than immersion and headspace SPME.

Authors+Show Affiliations

Department of Chemistry & Chemical Biology, Indiana University - Purdue University Indianapolis, Indianapolis, IN, USA.Forensic & Investigative Sciences, Indiana University - Purdue University Indianapolis, Indianapolis, IN, USA.Department of Chemistry & Chemical Biology, Indiana University - Purdue University Indianapolis, Indianapolis, IN, USA. Forensic & Investigative Sciences, Indiana University - Purdue University Indianapolis, Indianapolis, IN, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33400824

Citation

Davis, Kymeri E., et al. "Detection of Ɣ-hydroxybutyric Acid (GHB) and Ɣ-butyrolactone (GBL) in Alcoholic Beverages Via Total Vaporization Solid-phase Microextraction (TV-SPME) and Gas Chromatography-mass Spectrometry." Journal of Forensic Sciences, vol. 66, no. 3, 2021, pp. 846-853.
Davis KE, Hickey LD, Goodpaster JV. Detection of ɣ-hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) in alcoholic beverages via total vaporization solid-phase microextraction (TV-SPME) and gas chromatography-mass spectrometry. J Forensic Sci. 2021;66(3):846-853.
Davis, K. E., Hickey, L. D., & Goodpaster, J. V. (2021). Detection of ɣ-hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) in alcoholic beverages via total vaporization solid-phase microextraction (TV-SPME) and gas chromatography-mass spectrometry. Journal of Forensic Sciences, 66(3), 846-853. https://doi.org/10.1111/1556-4029.14660
Davis KE, Hickey LD, Goodpaster JV. Detection of Ɣ-hydroxybutyric Acid (GHB) and Ɣ-butyrolactone (GBL) in Alcoholic Beverages Via Total Vaporization Solid-phase Microextraction (TV-SPME) and Gas Chromatography-mass Spectrometry. J Forensic Sci. 2021;66(3):846-853. PubMed PMID: 33400824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of ɣ-hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) in alcoholic beverages via total vaporization solid-phase microextraction (TV-SPME) and gas chromatography-mass spectrometry. AU - Davis,Kymeri E, AU - Hickey,Logan D, AU - Goodpaster,John V, Y1 - 2021/01/05/ PY - 2020/11/30/revised PY - 2020/09/06/received PY - 2020/12/07/accepted PY - 2021/1/6/pubmed PY - 2021/6/30/medline PY - 2021/1/5/entrez KW - GBL KW - GHB KW - SPME KW - alcohol KW - drug facilitated sexual assault KW - total vaporization SP - 846 EP - 853 JF - Journal of forensic sciences JO - J Forensic Sci VL - 66 IS - 3 N2 - Total Vaporization Solid-Phase Microextraction (TV-SPME) relies on the same technique as standard SPME but completely vaporizes a sample extract, and analytes are sorbed directly from the vapor phase. On-fiber derivatization may also be performed using TV-SPME, where the fiber is first exposed to the headspace of a vial containing the derivatization agent, then exposed to a new vial containing the sample. ɣ-Hydroxybutyric acid (GHB) and ɣ-butyrolactone (GBL) are drugs of concern in that they may be used in drug facilitated sexual assault by surreptitiously spiking them into a victim's beverage. These drugs cause sedation, memory loss, and are difficult to detect in biological samples. One challenge in their analysis is that they can interconvert in aqueous samples, which was demonstrated in samples allowed to stand at room temperature for long periods. A volume study of GBL in water was performed with volumes ranging from 1 to 10,000 µl to compare the efficacy of TV-SPME, headspace SPME, and immersion SPME. Lastly, water, beer, wine, liquor, and mixed drinks were spiked with either GHB or GBL with realistic concentrations (mg/ml) and microliter quantities were analyzed using a TV-SPME Gas Chromatography-Mass Spectrometry method. The GBL volume study demonstrated an increased sensitivity in GBL detection when TV-SPME was utilized. Additionally, GHB and GBL were identified in various beverages at realistic concentrations. Overall, TV-SPME is beneficial because it requires no sample preparation and uses smaller sample volumes than immersion and headspace SPME. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/33400824/Detection_of_ɣ_hydroxybutyric_acid__GHB__and_ɣ_butyrolactone__GBL__in_alcoholic_beverages_via_total_vaporization_solid_phase_microextraction__TV_SPME__and_gas_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -