Tags

Type your tag names separated by a space and hit enter

The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection.
Int J Environ Res Public Health. 2021 01 02; 18(1)IJ

Abstract

COVID-19 patients always develop multiple organ dysfunction syndromes other than lungs, suggesting the novel virus SARS-CoV-2 also invades other organs. Therefore, studying the viral susceptibility of other organs is important for a deeper understanding of viral pathogenesis. Angiotensin-converting enzyme II (ACE2) is the receptor protein of SARS-CoV-2, and TMPRSS2 promotes virus proliferation and transmission. We investigated the ACE2 and TMPRSS2 expression levels of cell types from 31 organs to evaluate the risk of viral infection using single-cell RNA sequencing (scRNA-seq) data. For the first time, we found that the gall bladder and fallopian tube are vulnerable to SARS-CoV-2 infection. Besides, the nose, heart, small intestine, large intestine, esophagus, brain, testis, and kidney are also identified to be high-risk organs with high expression levels of ACE2 and TMPRSS2. Moreover, the susceptible organs are grouped into three risk levels based on the ACE2 and TMPRSS2 expression. As a result, the respiratory system, digestive system, and urinary system are at the top-risk level for SARS-CoV-2 infection. This study provides evidence for SARS-CoV-2 infection in the human nervous system, digestive system, reproductive system, respiratory system, circulatory system, and urinary system using scRNA-seq data, which helps in the clinical diagnosis and treatment of patients.

Authors+Show Affiliations

School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.School of Mathematics, Harbin Institute of Technology, Harbin 150001, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33401657

Citation

Qi, Jing, et al. "The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection." International Journal of Environmental Research and Public Health, vol. 18, no. 1, 2021.
Qi J, Zhou Y, Hua J, et al. The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection. Int J Environ Res Public Health. 2021;18(1).
Qi, J., Zhou, Y., Hua, J., Zhang, L., Bian, J., Liu, B., Zhao, Z., & Jin, S. (2021). The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection. International Journal of Environmental Research and Public Health, 18(1). https://doi.org/10.3390/ijerph18010284
Qi J, et al. The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection. Int J Environ Res Public Health. 2021 01 2;18(1) PubMed PMID: 33401657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The scRNA-seq Expression Profiling of the Receptor ACE2 and the Cellular Protease TMPRSS2 Reveals Human Organs Susceptible to SARS-CoV-2 Infection. AU - Qi,Jing, AU - Zhou,Yang, AU - Hua,Jiao, AU - Zhang,Liying, AU - Bian,Jialin, AU - Liu,Beibei, AU - Zhao,Zicen, AU - Jin,Shuilin, Y1 - 2021/01/02/ PY - 2020/10/29/received PY - 2020/12/21/revised PY - 2020/12/22/accepted PY - 2021/1/6/entrez PY - 2021/1/7/pubmed PY - 2021/1/12/medline KW - ACE2 KW - COVID-19 KW - SARS-CoV-2 KW - TMPRSS2 KW - scRNA-seq KW - susceptible organs JF - International journal of environmental research and public health JO - Int J Environ Res Public Health VL - 18 IS - 1 N2 - COVID-19 patients always develop multiple organ dysfunction syndromes other than lungs, suggesting the novel virus SARS-CoV-2 also invades other organs. Therefore, studying the viral susceptibility of other organs is important for a deeper understanding of viral pathogenesis. Angiotensin-converting enzyme II (ACE2) is the receptor protein of SARS-CoV-2, and TMPRSS2 promotes virus proliferation and transmission. We investigated the ACE2 and TMPRSS2 expression levels of cell types from 31 organs to evaluate the risk of viral infection using single-cell RNA sequencing (scRNA-seq) data. For the first time, we found that the gall bladder and fallopian tube are vulnerable to SARS-CoV-2 infection. Besides, the nose, heart, small intestine, large intestine, esophagus, brain, testis, and kidney are also identified to be high-risk organs with high expression levels of ACE2 and TMPRSS2. Moreover, the susceptible organs are grouped into three risk levels based on the ACE2 and TMPRSS2 expression. As a result, the respiratory system, digestive system, and urinary system are at the top-risk level for SARS-CoV-2 infection. This study provides evidence for SARS-CoV-2 infection in the human nervous system, digestive system, reproductive system, respiratory system, circulatory system, and urinary system using scRNA-seq data, which helps in the clinical diagnosis and treatment of patients. SN - 1660-4601 UR - https://www.unboundmedicine.com/medline/citation/33401657/The_scRNA_seq_Expression_Profiling_of_the_Receptor_ACE2_and_the_Cellular_Protease_TMPRSS2_Reveals_Human_Organs_Susceptible_to_SARS_CoV_2_Infection_ DB - PRIME DP - Unbound Medicine ER -