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Identification of anthocyanin compositions in black seed coated Korean adzuki bean (Vigna angularis) by NMR and UPLC-Q-Orbitrap-MS/MS and screening for their antioxidant properties using different solvent systems.
Food Chem. 2021 Jun 01; 346:128882.FC

Abstract

The aim of the present research was to investigate the antioxidant properties and anthocyanin profiles in the black seed coated adzuki bean (Vigna angularis, Geomguseul cultivar). The acidic 60% methanol extract (40 μg/mL) contains the highest total phenolic and flavonoid contents (486 ± 3 mg GAE/100 g; 314 ± 10 mg CE/100 g) with potent antioxidant properties (trolox equivalent 1272 ± 26 and 662 ± 24 mg TE/100 g) against ABTS and DPPH radicals compared to other methanol-water ratios (20, 40, 80, and 100%). Ten anthocyanin components were identified in this extract including delphinidin-3,5-O-digalactoside (1), delphinidin-3,5-O-diglucoside (2), delphinidin-3-O-galactoside (3), delphinidin-3-O-glucoside (4), delphinidin-3-O-rutinoside (5), delphinidin-3-O-(p-coumaroyl)glucoside (6), cyanidin-3-O-glucoside (7), petunidin-3-O-galactoside (8), petunidin-3-O-glucoside (9) and petunidin-3-O-(p-coumaroyl)glucoside (10) via NMR spectroscopy and UPLC-Q-Orbitrap-MS/MS analysis. The key anthocyanins 3 and 4 of delphinidin type were isolated by reversed phase C-18 MPLC. Our results indicate that the anthocyanin profiles as well as the high phenolic and flavonoid contents are important factors determining the antioxidant effects of black adzuki bean.

Authors+Show Affiliations

Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.Crop Foundation Division, National Institute of Crop Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, 20(th) Jeompiljero, Miryang 50424, Republic of Korea.Department of Life Resource Industry, Dong-A University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Republic of Korea. Electronic address: schem72@daum.net.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33412486

Citation

Ha, Tae Joung, et al. "Identification of Anthocyanin Compositions in Black Seed Coated Korean Adzuki Bean (Vigna Angularis) By NMR and UPLC-Q-Orbitrap-MS/MS and Screening for Their Antioxidant Properties Using Different Solvent Systems." Food Chemistry, vol. 346, 2021, p. 128882.
Ha TJ, Park JE, Lee KS, et al. Identification of anthocyanin compositions in black seed coated Korean adzuki bean (Vigna angularis) by NMR and UPLC-Q-Orbitrap-MS/MS and screening for their antioxidant properties using different solvent systems. Food Chem. 2021;346:128882.
Ha, T. J., Park, J. E., Lee, K. S., Seo, W. D., Song, S. B., Lee, M. H., Kim, S., Kim, J. I., Oh, E., Pae, S. B., Kwak, D. Y., & Lee, J. H. (2021). Identification of anthocyanin compositions in black seed coated Korean adzuki bean (Vigna angularis) by NMR and UPLC-Q-Orbitrap-MS/MS and screening for their antioxidant properties using different solvent systems. Food Chemistry, 346, 128882. https://doi.org/10.1016/j.foodchem.2020.128882
Ha TJ, et al. Identification of Anthocyanin Compositions in Black Seed Coated Korean Adzuki Bean (Vigna Angularis) By NMR and UPLC-Q-Orbitrap-MS/MS and Screening for Their Antioxidant Properties Using Different Solvent Systems. Food Chem. 2021 Jun 1;346:128882. PubMed PMID: 33412486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of anthocyanin compositions in black seed coated Korean adzuki bean (Vigna angularis) by NMR and UPLC-Q-Orbitrap-MS/MS and screening for their antioxidant properties using different solvent systems. AU - Ha,Tae Joung, AU - Park,Jae Eun, AU - Lee,Kwang-Sik, AU - Seo,Woo Duck, AU - Song,Seok-Bo, AU - Lee,Myoung-Hee, AU - Kim,Sungup, AU - Kim,Jung-In, AU - Oh,Eunyoung, AU - Pae,Suk-Bok, AU - Kwak,Do-Yeon, AU - Lee,Jin Hwan, Y1 - 2020/12/24/ PY - 2020/06/10/received PY - 2020/12/08/revised PY - 2020/12/10/accepted PY - 2021/1/8/pubmed PY - 2021/3/2/medline PY - 2021/1/7/entrez KW - Anthocyanin KW - Antioxidant activity KW - Black adzuki bean KW - Delphinidin-3-O-glucoside KW - NMR KW - Total flavonoid content KW - Total phenolic content KW - UPLC-Q-Orbitrap-MS/MS SP - 128882 EP - 128882 JF - Food chemistry JO - Food Chem VL - 346 N2 - The aim of the present research was to investigate the antioxidant properties and anthocyanin profiles in the black seed coated adzuki bean (Vigna angularis, Geomguseul cultivar). The acidic 60% methanol extract (40 μg/mL) contains the highest total phenolic and flavonoid contents (486 ± 3 mg GAE/100 g; 314 ± 10 mg CE/100 g) with potent antioxidant properties (trolox equivalent 1272 ± 26 and 662 ± 24 mg TE/100 g) against ABTS and DPPH radicals compared to other methanol-water ratios (20, 40, 80, and 100%). Ten anthocyanin components were identified in this extract including delphinidin-3,5-O-digalactoside (1), delphinidin-3,5-O-diglucoside (2), delphinidin-3-O-galactoside (3), delphinidin-3-O-glucoside (4), delphinidin-3-O-rutinoside (5), delphinidin-3-O-(p-coumaroyl)glucoside (6), cyanidin-3-O-glucoside (7), petunidin-3-O-galactoside (8), petunidin-3-O-glucoside (9) and petunidin-3-O-(p-coumaroyl)glucoside (10) via NMR spectroscopy and UPLC-Q-Orbitrap-MS/MS analysis. The key anthocyanins 3 and 4 of delphinidin type were isolated by reversed phase C-18 MPLC. Our results indicate that the anthocyanin profiles as well as the high phenolic and flavonoid contents are important factors determining the antioxidant effects of black adzuki bean. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/33412486/Identification_of_anthocyanin_compositions_in_black_seed_coated_Korean_adzuki_bean__Vigna_angularis__by_NMR_and_UPLC_Q_Orbitrap_MS/MS_and_screening_for_their_antioxidant_properties_using_different_solvent_systems_ DB - PRIME DP - Unbound Medicine ER -