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Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone.
BMC Genomics. 2021 Jan 07; 22(1):34.BG

Abstract

BACKGROUND

Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment.

RESULTS

Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99-23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci.

CONCLUSIONS

Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

Links

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Authors+Show Affiliations

Wang Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Yu C
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Cheng Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Yao F
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Long L
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Wu Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Li J
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Li H
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Wang J
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Jiang Q
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Li W
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Pu Z
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Qi P
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Ma J
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Deng M
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Wei Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Chen X
US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit; and Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA.
Chen G
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China.
Kang H
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. houyang.kang@sicau.edu.cn.
Jiang Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. jiangyunfeng@sicau.edu.cn.
Zheng Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P. R. China. ylzheng@sicau.edu.cn.

MeSH

BasidiomycotaChinaDisease ResistanceGenome-Wide Association StudyPlant DiseasesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33413106

Citation

Wang, Yuqi, et al. "Genome-wide Association Mapping Reveals Potential Novel Loci Controlling Stripe Rust Resistance in a Chinese Wheat Landrace Diversity Panel From the Southern Autumn-sown Spring Wheat Zone." BMC Genomics, vol. 22, no. 1, 2021, p. 34.
Wang Y, Yu C, Cheng Y, et al. Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. BMC Genomics. 2021;22(1):34.
Wang, Y., Yu, C., Cheng, Y., Yao, F., Long, L., Wu, Y., Li, J., Li, H., Wang, J., Jiang, Q., Li, W., Pu, Z., Qi, P., Ma, J., Deng, M., Wei, Y., Chen, X., Chen, G., Kang, H., ... Zheng, Y. (2021). Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. BMC Genomics, 22(1), 34. https://doi.org/10.1186/s12864-020-07331-1
Wang Y, et al. Genome-wide Association Mapping Reveals Potential Novel Loci Controlling Stripe Rust Resistance in a Chinese Wheat Landrace Diversity Panel From the Southern Autumn-sown Spring Wheat Zone. BMC Genomics. 2021 Jan 7;22(1):34. PubMed PMID: 33413106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. AU - Wang,Yuqi, AU - Yu,Can, AU - Cheng,Yukun, AU - Yao,Fangjie, AU - Long,Li, AU - Wu,Yu, AU - Li,Jing, AU - Li,Hao, AU - Wang,Jirui, AU - Jiang,Qiantao, AU - Li,Wei, AU - Pu,Zhien, AU - Qi,Pengfei, AU - Ma,Jian, AU - Deng,Mei, AU - Wei,Yuming, AU - Chen,Xianming, AU - Chen,Guoyue, AU - Kang,Houyang, AU - Jiang,Yunfeng, AU - Zheng,Youliang, Y1 - 2021/01/07/ PY - 2020/04/08/received PY - 2020/12/15/accepted PY - 2021/1/8/entrez PY - 2021/1/9/pubmed PY - 2021/5/15/medline KW - Chinese wheat landrace KW - GWAS KW - Southern China KW - Stripe rust resistance SP - 34 EP - 34 JF - BMC genomics JO - BMC Genomics VL - 22 IS - 1 N2 - BACKGROUND: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant cultivars are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions. In addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under a controlled greenhouse environment. RESULTS: Seventeen accessions showed stable high-level resistance to stripe rust across all environments in the field tests. Four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers that covered the entire genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99-23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, whereas four and one QTL conferring seedling and adult-plant resistance, respectively, were mapped distantly from previously reported stripe rust resistance genes or QTL and thus may be novel resistance loci. CONCLUSIONS: Our results provided an integrated overview of stripe rust resistance resources in a wheat landrace diversity panel from the southern autumn-sown spring wheat zone of China. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/33413106/Genome_wide_association_mapping_reveals_potential_novel_loci_controlling_stripe_rust_resistance_in_a_Chinese_wheat_landrace_diversity_panel_from_the_southern_autumn_sown_spring_wheat_zone_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓5 ↑71]

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