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Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies.
Vaccine. 2021 02 05; 39(6):1000-1006.V

Abstract

BACKGROUND

Micrurus venoms contain two main groups of toxic protein components: short-chain α-neurotoxins (SNtx) and phospholipases type A2 (PLA2). In North America, generally, the Micrurus venoms have low abundance of SNtx compared to that of PLA2s; however, both are highly toxic to mammals, and consequently both can play a major role in the envenomation processes. Concerning the commercial horse-derived antivenoms against Micrurus from the North America region, they contain a relatively large amount of antibodies against PLA2s, and a low content of antibodies against short chain α-neurotoxins. This is mainly due to the lower relative abundance of SNtxs, and also to its poor immunogenicity due to their size and nature. Hence, Micrurus antivenoms made in North America usually present low neutralizing capacity towards Micrurus venoms whose lethality depend largely on short chain α-neurotoxins, such as South American Micrurus species.

METHODS

Horses were hyperimmunized with either the venom of M. tener (PLA2-predominant) or a recombinant short-chain consensus α-neurotoxin (ScNtx). Then, the combination of the two monospecific horse antibodies (anti-M. tener and anti-ScNtx) was used to test their efficacy against eleven Micrurus venoms.

RESULTS

The blend of anti-M. tener and anti-ScNtx antibodies had a better capacity to neutralize the lethality of diverse species from North, Central and South American Micrurus venoms. The antibodies combination neutralized both the ScNtx and ten out of eleven Micrurus venom tested, and particularly, it neutralized the venoms of M. distans and M. laticollaris that were neither neutralized by monospecific anti-M. tener nor anti-ScNtx.

CONCLUSIONS

These results provide a proof-of-principle for using recombinant immunogens to enrich poor or even non-neutralizing antisera against elapid venoms containing short chain α-neurotoxins to develop antivenoms with higher effectiveness and broader neutralizing capacity.

Authors+Show Affiliations

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico.The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S3E1, Canada. Electronic address: g.delarosa@utoronto.ca.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico.Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico. Electronic address: corzo@ibt.unam.mx.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33423840

Citation

Archundia, Irving G., et al. "Assessment of Neutralization of Micrurus Venoms With a Blend of anti-Micrurus Tener and anti-ScNtx Antibodies." Vaccine, vol. 39, no. 6, 2021, pp. 1000-1006.
Archundia IG, de la Rosa G, Olvera F, et al. Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies. Vaccine. 2021;39(6):1000-1006.
Archundia, I. G., de la Rosa, G., Olvera, F., Calderón, A., Benard-Valle, M., Alagón, A., & Corzo, G. (2021). Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies. Vaccine, 39(6), 1000-1006. https://doi.org/10.1016/j.vaccine.2020.12.052
Archundia IG, et al. Assessment of Neutralization of Micrurus Venoms With a Blend of anti-Micrurus Tener and anti-ScNtx Antibodies. Vaccine. 2021 02 5;39(6):1000-1006. PubMed PMID: 33423840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of neutralization of Micrurus venoms with a blend of anti-Micrurus tener and anti-ScNtx antibodies. AU - Archundia,Irving G, AU - de la Rosa,Guillermo, AU - Olvera,Felipe, AU - Calderón,Arlene, AU - Benard-Valle,Melisa, AU - Alagón,Alejandro, AU - Corzo,Gerardo, Y1 - 2021/01/08/ PY - 2020/04/20/received PY - 2020/12/11/revised PY - 2020/12/14/accepted PY - 2021/1/12/pubmed PY - 2021/5/22/medline PY - 2021/1/11/entrez KW - Antivenoms KW - Coral snakes KW - Elapid snakes KW - Micrurus venoms KW - Phospholipase A(2) KW - Short-chain α-neurotoxin KW - Snake antivenoms KW - Snakebites SP - 1000 EP - 1006 JF - Vaccine JO - Vaccine VL - 39 IS - 6 N2 - BACKGROUND: Micrurus venoms contain two main groups of toxic protein components: short-chain α-neurotoxins (SNtx) and phospholipases type A2 (PLA2). In North America, generally, the Micrurus venoms have low abundance of SNtx compared to that of PLA2s; however, both are highly toxic to mammals, and consequently both can play a major role in the envenomation processes. Concerning the commercial horse-derived antivenoms against Micrurus from the North America region, they contain a relatively large amount of antibodies against PLA2s, and a low content of antibodies against short chain α-neurotoxins. This is mainly due to the lower relative abundance of SNtxs, and also to its poor immunogenicity due to their size and nature. Hence, Micrurus antivenoms made in North America usually present low neutralizing capacity towards Micrurus venoms whose lethality depend largely on short chain α-neurotoxins, such as South American Micrurus species. METHODS: Horses were hyperimmunized with either the venom of M. tener (PLA2-predominant) or a recombinant short-chain consensus α-neurotoxin (ScNtx). Then, the combination of the two monospecific horse antibodies (anti-M. tener and anti-ScNtx) was used to test their efficacy against eleven Micrurus venoms. RESULTS: The blend of anti-M. tener and anti-ScNtx antibodies had a better capacity to neutralize the lethality of diverse species from North, Central and South American Micrurus venoms. The antibodies combination neutralized both the ScNtx and ten out of eleven Micrurus venom tested, and particularly, it neutralized the venoms of M. distans and M. laticollaris that were neither neutralized by monospecific anti-M. tener nor anti-ScNtx. CONCLUSIONS: These results provide a proof-of-principle for using recombinant immunogens to enrich poor or even non-neutralizing antisera against elapid venoms containing short chain α-neurotoxins to develop antivenoms with higher effectiveness and broader neutralizing capacity. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/33423840/Assessment_of_neutralization_of_Micrurus_venoms_with_a_blend_of_anti_Micrurus_tener_and_anti_ScNtx_antibodies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(20)31632-7 DB - PRIME DP - Unbound Medicine ER -