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Biogenic Silver Nanoparticles Synthesized by Lysinibacillus xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio parahaemolyticus and Salmonella Typhimurium.
Front Bioeng Biotechnol. 2020; 8:597502.FB

Abstract

The present study highlights a simple and eco-friendly method for the biosynthesis of silver nanoparticles (AgNPs) using Lysinibacillus xylanilyticus strain MAHUQ-40. Also, the synthesized AgNPs were used to investigate their antibacterial activity and mechanisms against antibiotic-resistant pathogens. Biosynthesis of AgNPs was confirmed by ultraviolet-visible spectroscopy, and then, they were characterized by field emission-transmission electron microscopy (FE-TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and fourier transform-infrared (FTIR). The toxicity of AgNPs against two pathogenic bacteria was evaluated. The UV-vis spectral scanning showed the peak for synthesized AgNPs at 438 nm. Under FE-TEM, the synthesized AgNPs were spherical with diameter ranges from 8 to 30 nm. The XRD analysis revealed the crystallinity of synthesized AgNPs. FTIR data showed various biomolecules including proteins and polysaccharides that may be involved in the synthesis and stabilization of AgNPs. The resultant AgNPs showed significant antibacterial activity against tested pathogens. The MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations) of the AgNPs synthesized by strain MAHUQ-40 were 3.12 and 12.5 μg/ml, respectively, against Vibrio parahaemolyticus and 6.25 and 25 μg/ml, respectively, against Salmonella Typhimurium. FE-TEM analysis showed that the biogenic AgNPs generated structural and morphological changes and damaged the membrane integrity of pathogenic bacteria. Our findings showed the potentiality of L. xylanilyticus MAHUQ-40 to synthesis AgNPs that acted as potent antibacterial material against pathogenic bacterial strains.

Authors+Show Affiliations

Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong, South Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33425864

Citation

Huq, Md Amdadul. "Biogenic Silver Nanoparticles Synthesized By Lysinibacillus Xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio Parahaemolyticus and Salmonella Typhimurium." Frontiers in Bioengineering and Biotechnology, vol. 8, 2020, p. 597502.
Huq MA. Biogenic Silver Nanoparticles Synthesized by Lysinibacillus xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio parahaemolyticus and Salmonella Typhimurium. Front Bioeng Biotechnol. 2020;8:597502.
Huq, M. A. (2020). Biogenic Silver Nanoparticles Synthesized by Lysinibacillus xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio parahaemolyticus and Salmonella Typhimurium. Frontiers in Bioengineering and Biotechnology, 8, 597502. https://doi.org/10.3389/fbioe.2020.597502
Huq MA. Biogenic Silver Nanoparticles Synthesized By Lysinibacillus Xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio Parahaemolyticus and Salmonella Typhimurium. Front Bioeng Biotechnol. 2020;8:597502. PubMed PMID: 33425864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biogenic Silver Nanoparticles Synthesized by Lysinibacillus xylanilyticus MAHUQ-40 to Control Antibiotic-Resistant Human Pathogens Vibrio parahaemolyticus and Salmonella Typhimurium. A1 - Huq,Md Amdadul, Y1 - 2020/12/16/ PY - 2020/08/21/received PY - 2020/11/17/accepted PY - 2021/1/11/entrez PY - 2021/1/12/pubmed PY - 2021/1/12/medline KW - AgNPs KW - Lysinibacillus xylanilyticus MAHUQ-40 KW - antimicrobial activity KW - eco-friendly synthesis KW - human pathogens SP - 597502 EP - 597502 JF - Frontiers in bioengineering and biotechnology JO - Front Bioeng Biotechnol VL - 8 N2 - The present study highlights a simple and eco-friendly method for the biosynthesis of silver nanoparticles (AgNPs) using Lysinibacillus xylanilyticus strain MAHUQ-40. Also, the synthesized AgNPs were used to investigate their antibacterial activity and mechanisms against antibiotic-resistant pathogens. Biosynthesis of AgNPs was confirmed by ultraviolet-visible spectroscopy, and then, they were characterized by field emission-transmission electron microscopy (FE-TEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and fourier transform-infrared (FTIR). The toxicity of AgNPs against two pathogenic bacteria was evaluated. The UV-vis spectral scanning showed the peak for synthesized AgNPs at 438 nm. Under FE-TEM, the synthesized AgNPs were spherical with diameter ranges from 8 to 30 nm. The XRD analysis revealed the crystallinity of synthesized AgNPs. FTIR data showed various biomolecules including proteins and polysaccharides that may be involved in the synthesis and stabilization of AgNPs. The resultant AgNPs showed significant antibacterial activity against tested pathogens. The MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations) of the AgNPs synthesized by strain MAHUQ-40 were 3.12 and 12.5 μg/ml, respectively, against Vibrio parahaemolyticus and 6.25 and 25 μg/ml, respectively, against Salmonella Typhimurium. FE-TEM analysis showed that the biogenic AgNPs generated structural and morphological changes and damaged the membrane integrity of pathogenic bacteria. Our findings showed the potentiality of L. xylanilyticus MAHUQ-40 to synthesis AgNPs that acted as potent antibacterial material against pathogenic bacterial strains. SN - 2296-4185 UR - https://www.unboundmedicine.com/medline/citation/33425864/Biogenic_Silver_Nanoparticles_Synthesized_by_Lysinibacillus_xylanilyticus_MAHUQ_40_to_Control_Antibiotic_Resistant_Human_Pathogens_Vibrio_parahaemolyticus_and_Salmonella_Typhimurium_ L2 - https://doi.org/10.3389/fbioe.2020.597502 DB - PRIME DP - Unbound Medicine ER -
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